Comparative Studies on Protoplast Regeneration in Herbaceous Species of the Dicotyledoneae Class

Binding, Horst; Nehls, Reinhard; Kock, R. Logan; Finger, J. W.; Mordhorst, G.

doi:10.1016/s0044-328x(81)80046-3

Cited by 100 publications

(25 citation statements)

References 25 publications

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“…First divisions of mesophyll protoplasts of Cichorium intybus L. and C. endivia L. were reported by Binding et al (1981), but protoplast development and plant regeneration were problematical for the various lines and hybrids tested (Crepy et al 1982). Fertile plants were obtained in our laboratory from C. intybus 'Zoom', a Witloof chicory (Saksi et al 1986) and from C. intybus 'P6v61e', an industrial chicory (Rambaud et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from protoplasts of Cichorium intybus L. x Cichorium endivia L.

Sidikou-Seyni

Rambaud

Dubois

et al. 1992

Plant Cell Tiss Organ Cult

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Somatic embryos and adult plants were regenerated from mesophyll protoplasts of a clone of chicory '474' (Cichorium intybus L. x Cichorium endivia L.). Embryos were obtained in three different ways: -plating of 7-day-old protoplast cultures on PM-3 solid induction medium with 2.5 IxM 2-isopentenyladenine (2-iP) and 0.5 I~M naphthaleneacetic acid (NAA); -transfer of 1-2 mm microcolonies into liquid M-17 induction medium with 2.5 ixM (2-iP) and 0.1 ixM (NAA); -transfer of well-established protoplast-derived calluses onto M-17. Development of embryos was accomplished in agitated liquid Heller medium with 0.15 t~M gibberellic acid and germination on solid Heller medium without growth regulators. The total time for plantlet (4-1eaves stage) recovery, following protoplast isolation, was 13 to 15 weeks. After acclimatization all protoplast-derived plants of Cichorium '474' were phenotypically normal and fertile.

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Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from protoplasts of Cichorium intybus L. x Cichorium endivia L.

Sidikou-Seyni

Rambaud

Dubois

et al. 1992

Plant Cell Tiss Organ Cult

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“…With sunflower protoplast cultures, callus formation has been achieved in a few cases [8,9,10], since the first brief report on shoot formation [11].…”

Section: Introductionmentioning

confidence: 99%

Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.

Dupuis¹,

Péan²,

Chagvardieff

1990

Plant Cell Tiss Organ Cult

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The effects of factors influencing sunflower protoplast isolation yield, plating efficiency (PE) and the early differentiation into embryoids (embryogenic capacity, EC) have been studied. Only hypocotyl-derived protoplasts divided. The variations of PE and EC in the various treatments did not seem to be linked to the protoplast yields. From statistical analysis of the data, we concluded that, the sunflower genotype, the age and height of seedlings, the part of hypocotyl used, the incubation time (from 6 to 16 hours) in enzymes of explants or of protoplasts alone, influenced PE but large variations were detected for EC. A comparison of the factors effecting EC suggested an origin, inside the hypocotyl, of cells able to give rise, after induction, to embryogenic protoplasts.

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“…Some authors have proved the regeneration ability of protoplasts obtained from Nigella and reported on the easy accessibility of Nigella for this technique as well (Iha and Roy 1979;Binding et al 1981). Protoplasts isolated from a suspension culture of N. sativa regenerated cell walls and started to proliferate within 48 h. Subsequently, friable callus masses were formed.…”

Section: Establishment Characterization and Differentiationmentioning

confidence: 99%

Nigella spp.: In Vitro Culture, Regeneration, and the Formation of Secondary Metabolites

Schmauder¹,

Doebel²

1991

Biotechnology in Agriculture and Forestry

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Comparative Studies on Protoplast Regeneration in Herbaceous Species of the Dicotyledoneae Class

Cited by 100 publications

References 25 publications

Somatic embryogenesis and plant regeneration from protoplasts of Cichorium intybus L. x Cichorium endivia L.

Somatic embryogenesis and plant regeneration from protoplasts of Cichorium intybus L. x Cichorium endivia L.

Plant donor tissue and isolation procedure effect on early formation of embryoids from protoplasts of Helianthus annuus L.

Nigella spp.: In Vitro Culture, Regeneration, and the Formation of Secondary Metabolites

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