Comparative studies on the purity and specificity of yolk immunoglobulin Y isolated from eggs laid by hens immunized with toxoplasma gondii antigen

Hassl, Andreas; Aspöck, Horst; Flamm, H

doi:10.1016/s0176-6724(87)80009-3

Cited by 13 publications

(12 citation statements)

References 6 publications

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“…Hassl and Aspock (1987) produced yolk IgY -antibodies against Toxoplasma gondii and used the PEG method according to Polson et al (1980) for isolation. For purifying the yolk-IgY, the authors set a sephacryl S-300 column (1.6 x 60 cm), to which they applied 2 ml of the IgY fraction dissolved in PBS.…”

Section: Resultsmentioning

confidence: 99%

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Isolation of IgY from Yolk

Staak

Schwarzkopf

Behn

et al. 2001

Chicken Egg Yolk Antibodies, Production and Application

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Section: Resultsmentioning

confidence: 99%

“…Also mentioned are the virus neutralisation test (VNT) (Czerny and Eichhorn, 1989), the indirect haemagglutination test (IHA) (Hassl and Aspock, 1987), the radio-immuno-assay (RIA) (Rosoi et al, 1993) and immuno-fluorescence-technique (FAT, IFAT) (Schmidt et al, 1989;Gervelmeyer, 1997).…”

Section: Resultsmentioning

confidence: 99%

Isolation of IgY from Yolk

Staak

Schwarzkopf

Behn

et al. 2001

Chicken Egg Yolk Antibodies, Production and Application

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“…Hassl et al [108] first described the production of anti-T. gondii IgY. They used total soluble antigens that are immunogenic to comparatively produce rabbit IgG and chicken IgY.…”

Section: State Of Art the Use Of Igy For Toxoplasma Gondii Studiesmentioning

confidence: 99%

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Júnior¹,

Santos²,

Bassi³

et al. 2017

Toxoplasmosis

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In this chapter, we describe relevant aspects of immunoglobulin Y (IgY) technology for Toxoplasma gondii applications, including comparison of avian IgY antibody with mammalian IgG antibody, egg yolk IgY production and isolation procedures, important applications for IgY antibody, and state of the art and perspectives for IgY-technology in T. gondii studies. T. gondii is a worldwide public health problem. IgY-technology provides an alternative antibody (IgY) to mammalian Immunoglobulin G (IgG) antibody. IgY-technology involves the chicken immunization, yolk IgY isolation, antibody characterization, and purified IgY application to several kinds of methods. Immunized chicken transfers a specific IgY from blood to egg yolk. Phylogenetic distance between chickens and mammals influences the generation of antibody repertoires recognizing an antigen profile. IgY is not bound to rheumatoid factor or mammalian complement protein and thus avoids the false-positive results. Yolk IgY isolation is carried out by simple procedures that are accessible for any laboratory and, also, for IgY isolation at large-scale production. IgY-technology provides antibodies for proteomic studies, diagnostic assays, and immunotherapy. Although IgY-technology is promising, there is a reduced number of investigations with IgY and T. gondii. Future perspectives involve the use of IgY-technology for the screening of new T. gondii antigens for diagnostics, therapy, or vaccine, development of innovative techniques for toxoplasmosis diagnostics and may be an immunotherapy for toxoplasmosis.

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“…Different purifications of IgYs have been described, based on propanol or polycthyleneglycol (PEG) precipitation, sometimes com bined with hydrophobic chromatography [7][8][9]. We used the PEG 6000 sequential precipi tation method of Poison et al [2] associated with a final 2.3 M (N H^SC^ precipitation.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic Studies on Molecular Defects of von Willebrand Factor and Platelet Glycoprotein llb-llla with Antibodies Produced in Egg Yolk from Laying Hens

Toti

Gachet²,

Ohlmann³

et al. 1992

Pathophysiol Haemos Thromb

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Immunoglobulins isolated from egg yolk (IgYs) are a convenient source of polyclonal antibodies. Their purification is simple and the yields important (50 mg immunoglobulins/egg). Nevertheless their biochemical characteristics are different from those of rabbit antibodies generally used for the study of molecular defects of plasmatic and platelet proteins. Provided standardization is achieved, IgYs can be used for immunoelectrophoresis, immunoprecipitation or immunoblotting assays. We describe here the electrophoretic and immunoblotting conditions employed to explore human plasmatic and platelet von Willebrand factor (vWF) and human platelet GP Ilb-IHa using IgYs. These two proteins are involved in primary hemostasis and their absence or abnormality is responsible for hereditary bleeding disorders. The methods were applied to the characterization of patients with vWF or GP Ilb-IIIa defects and compared to classical mammalian IgG immunoelectrophoretic techniques. Results were further confirmed by flow cytometric analysis.

show abstract

Comparative studies on the purity and specificity of yolk immunoglobulin Y isolated from eggs laid by hens immunized with toxoplasma gondii antigen

Cited by 13 publications

References 6 publications

Isolation of IgY from Yolk

Isolation of IgY from Yolk

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Electrophoretic Studies on Molecular Defects of von Willebrand Factor and Platelet Glycoprotein llb-llla with Antibodies Produced in Egg Yolk from Laying Hens

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