Comparative Studies on Tobacco Pith and Sweet Potato Root Isoperoxidases in Relation to Injury, Indoleacetic Acid, and Ethylene Effects

Birecka, Helen; Briber, K. A.; Catalfamo, James L.

doi:10.1104/pp.52.1.43

Cited by 59 publications

(30 citation statements)

References 21 publications

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“…Thus, repression of A2 by auxins might be due to auxin-induced ethylene synthesis, but auxin itself must have been responsible for repression of the other isoperoxidases. This conclusion has also been independently reached by Birecka et al (2).…”

supporting

confidence: 73%

“…Decreases in peroxidase activity following ethylene have also been noted in one isoperoxidase of whole cotton seedlings (11) and in total peroxidase activity of sweet potato (14). The lack of ethylene (2). Since the activity of the constitutive isoperoxidases in pith can be enhanced by ethylene, it is reasonable to suppose that cathodic and slowly moving anodic isoperoxidases, which are constitutive in other parts of the tobacco plant (8), can be enchanced by ethylene application in situ.…”

mentioning

confidence: 99%

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Differential Effects of Ethylene on Pith Peroxidase of Intact Tobacco Plants and Excised Tissue

Adams

Galston²

1974

Plant Physiol.

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Ethylene increases the pith peroxidase activity of intact tobacco plants (Nicotiana tabacum) but not of excised pith, either at atmospheric or reduced pressures. In the intact plant, the increased activity involves augmentation of the two constitutive anodic isoperoxidases. In the excised pith, ethylene strongly represses one injury-induced isoperoxidase, while not markedly affecting other isozymes known to be repressed by auxin. Thus, the previously described auxin-induced repression of peroxidase is not due mainly to auxin-induced ethylene formation.Freshly excised tobacco pith has low peroxidase activity, attributable mainly to a rapidly moving group of constitutive anodic isozymes. Following excision and aseptic culture of the pith, new cathodic and slowly moving anodic isoperoxidases appear and increase rapidly in activity. Treatment of excised pith with physiological levels of auxins largely inhibits the appearance and increase of these new isoperoxidases (3,8 RESULTS AND DISCUSSIONConfirming the results of numerous authors with many plant materials (1), ethylene treatment of intact tobacco plants resulted in a large enhancement of total peroxidase activity in the pith of upper, middle, and lower internodes by all concentrations of ethylene employed (Fig. 1)

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supporting

confidence: 73%

mentioning

confidence: 99%

Differential Effects of Ethylene on Pith Peroxidase of Intact Tobacco Plants and Excised Tissue

Adams

Galston²

1974

Plant Physiol.

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“…In experiments, in which vacuum-infiltration was applied before freezing to isolate the free wall peroxidase, additional samples were taken which were frozen without being infiltrated. Peroxidase determination, electrophoresis, and isoperoxidase scanning were carried out also as previously (3).…”

Section: Methodsmentioning

confidence: 99%

Cell Wall and Protoplast Isoperoxidases in Tobacco Plants in Relation to Mechanical Injury and Infection with Tobacco Mosaic Virus

Birecka¹,

Catalfamo²,

Urban³

1975

Plant Physiol.

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Leaves and pith of Turkish, Wisconsin 38, and Samsun NN tobacco (Nicotiana tabacum) varieties, which differ in their sensitivity to tobacco mosaic virus, showed the same qualitative isoperoxidase patterns and a similar distribution of distinctive isoperoxidases between the cell protoplast and wall-free, ionically, and covalently bound fractions. No changes in the qualitative isoenzyme spectrum were found in relation to age, mechanical injury, or leaf infection with tobacco mosaic virus. The distinctive isoperoxidases which reacted to infection were the same as those responsive to mechanical injury, confirming that the enzyme reaction to infection results from a nonspecific response to injury. The increase in peroxidase activity in response to infection or mechanical injury, or both, was greater in young tissue than in the older ones. The great increase in Samsun NN leaves and no increase in those of the two other varieties in response to infection may be due to differences in the degree to which the pathogen affected processes controlling the nonspecific peroxidase reaction to injury. Peroxidase development in the infected Samsun NN leaves was due to isoenzymes which form the wall-bound fraction in very young tissues, and to those which increase in activity with aging in the protoplast and wall-free fractions. In mechanically injured tissue, only the first group of isoenzymes increased in activity. In Samsun NN plants, the increased peroxidase activity in upper intact leaves above the infected ones was only due to isoenzymes whose activity increases with both normal and virus-accelerated senescence. Peroxidase reaction to challenge inoculation in these leaves was the same whether the lower ones were intact, infected and/or mechanically injured. Thus, the induced systemic resistance to tobacco mosaic virus may be due to other than peroxidase factors.In infected tissues, peroxidase was detected in the endoplas. mic reticulum, Golgi apparatus, vacuole, cell wall, and intercellular spaces. The Golgi vesicles were often localized near the tonoplast and plasmalemma, fusing with membranes and secreting their contents. The possible "rejuvenating" effects of injury on synthesis and transport of distinctive isoperoxidases are discussed.eases. Systemic-induced resistance to TMV' in the hypersensitive Samsun NN tobacco plants (9) has been also related to peroxidase; increased peroxidase activity leading to production of toxic compounds causes early killing of infected cells and early formation of a barrier to viral spread (30, 31). Increases in the activity of peroxidase in infected organs, usually referred to the soluble fraction of this enzyme, are assumed by many to result from a nonspecific host response (2,11,18,27,32,34), although new soluble isoperoxidases are claimed to appear in some infected tissues (2,8,10,11,26).Previous study on Wisconsin 38 tobacco plant pith has shown a great increase of peroxidase activity, not only in its protoplast fraction, but also in the wall-bound fraction in response to cuttin...

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“…POD activity was calculated using the extinction coefficient 25.5 mM −1 cm −1 . One unit activity was defined as an increase of 1 A min -1 (Birecka et al, 1973;Doganlar and Atmaca, 2011). Total POD activity was expressed as ∆A/g F.W.…”

Section: Determination Of Total Soluble Protein Contentmentioning

confidence: 99%

Metal accumulation and physiological responses induced by copper and cadmium in Lemna gibba, L. minor and Spirodela polyrhiza

Doğanlar

2013

Chemical Speciation & Bioavailability

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Comparative Studies on Tobacco Pith and Sweet Potato Root Isoperoxidases in Relation to Injury, Indoleacetic Acid, and Ethylene Effects

Cited by 59 publications

References 21 publications

Differential Effects of Ethylene on Pith Peroxidase of Intact Tobacco Plants and Excised Tissue

Differential Effects of Ethylene on Pith Peroxidase of Intact Tobacco Plants and Excised Tissue

Cell Wall and Protoplast Isoperoxidases in Tobacco Plants in Relation to Mechanical Injury and Infection with Tobacco Mosaic Virus

Metal accumulation and physiological responses induced by copper and cadmium in Lemna gibba, L. minor and Spirodela polyrhiza

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