Comparative study in swines' vocal cords healing after excision of fragment with CO2 laser with mitomycin and 5-fluorouracil postoperative topical application

Abstract

PURPOSE: To evaluate the deposition of collagen fibers at pig's vocal folds after topical use of mitomycin or 5-fluorouracil, when partial exeresis of mucosa layer had been promoted by CO2 laser. METHODS: There were used 18 Larger white pigs which were anesthetized and submitted to mucosa fragment's exeresis, bilaterally, at its free border. The animals were divided into 3 groups, each one with 6 animals: control group, without topical drug application; mitomycin group; and 5-fluorouracil group. After 30 days, the animals were subjected to euthanasia, and samples of the vocal folds were collected and stained by picrosirius red technique with polarization for quantification of total collagen deposition. RESULTS: In control group, the mean rate of right vocal fold's collagen deposition at submucosa consisted in a 3428.66 micrometers area. There was found an area whose size had, in average, 2196.36 micrometers, in mitomycin group, and 2269.19 micrometers, in 5-fluorouracil group. CONCLUSION: Mitomycin and 5-fluorouracil had promoted beneficial change in vocal fold's cicatrization with less collagen deposition, but there was no significant statistically difference when they were compared between themselves.

Effects of the application of the amniotic membrane in the healing process of skin wounds in rats

“…We emphasize that the use of the HAM offers the advantages of high availability, ease of application, and good storability [12][13][14][15][16][17][18] . In this context, it is possible to affirm that by presenting properties that favor the re-structuring of the damaged portions of the tissues, the amniotic membrane could be used as a biological coverage of acute and chronic wounds [18][19][20][21][22][23][24][25][26] .…”

“…The proliferative phase of healing occurs 3 to 5 days after tissue injury and is characterized by an increase in the number of fibroblasts, which produce and deposit a large amount of type III collagen. Later, the maturation of these fibers occurs, with their remodeling and replacement by type I collagen, which is thicker and more resistant 25 . The present study demonstrates that the collagen fibers of the group T animals reached maturity and early macromolecular orientation compared with that of the control animals, which had a slower wound healing process.…”

Comparative histological study on wound healing on rat's skin treated with Mitomycin C or Clobetasol propionate

“…The findings from the present study therefore indicate that administration of MMC and CP at the specified dosages and posology did not alter the angiogenesis at the times of 30 and 60 days. On the other hand, Ribeiro et al 7 demonstrated that there was a significant increase in vascular proliferation in scar fragments on the backs of rats, in a group treated with topical MMC at a concentration of 0.5 mg/ml for 5 minutes, after a period of 90 days.A decrease in the collagen concentration in scar fragments from the vocal folds of pigs stained with picrosirius red was observed by Camargo et al17 and Baptistella et al8 . Both of these authors applied MMC soaked in cotton to the wound for three minutes and analyzed the effect of the medication on healing after 30 days.…”

“…Both of these authors applied MMC soaked in cotton to the wound for three minutes and analyzed the effect of the medication on healing after 30 days. However, Camargo et al17 used MMC at a concentration of 0.4 mg/ml and Baptistella et al8 , 0.04%.No other studies demonstrating inversion of the concentration of collagen fibers in scars between 30 and 60 days,…”

The Effect of Ascorbic Acid Supplementation on the Time of Healing of Rats Submitted to Neurosurgical Procedures

“…The present study was based on previous works, cited in the bibliographic reference, 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 for the choice and organization of groups, surgical technique, dose of ascorbic acid, technique for evaluating the inflammatory process and intensity of healing, which observed a greater number and better arrangement of fibroblasts in animals in groups II and III when compared with the control group, since the use of ascorbic acid maintains an adequate concentration of the vitamin in the skin, which stimulates the proliferation of dermal fibroblasts.…”