Comparative Study of Antibacterial Activity of Juice and Peel Extract of Citrus Fruits

Abstract

ABSRTACTObjectives: This study was aimed to compare the antibacterial activity of peel and juice extracts of citrus fruits.Methods: The antibacterial activity of juice and peel extract of fi ve selected citrus fruits [C1-Citrus reticulata (mandarin orange), C2-Citrus limetta (sweet lime), C3-Citrus aurantifolia (lime), C4-Citrus limon (lemon) and C5-Citrus maxima (pommelo)] on three Gram positive and fi ve Gram negative bacterial strains were examined. The juice and peel extract of citrus fruits was obtained by using absolute ethanol. The obtained extracts were dissolved in 1ml 10% v/v DMSO which was taken to carry out the antibacterial susceptibility assay using agar well diffusion method. Results:The peel extracts of C1 and C2 were found to be more effective against the given Gram-positive bacteria than the juice extracts with maximum zone of inhibition (20.33±1.527) and (15.33±0.577) against Bacillus spp respectively. While the juice extracts of C3 and C4 were more effective against both Gram positive and negative bacteria than their peel extracts with maximum zone of inhibition (26.66±1.15) against S. aureus ATCC 25923 and (20±1) against E. coli ATCC 25922 respectively. The juice extract of C5 was found to be more effective against both Gram positive and negative bacteria than its peel extract with maximum zone of inhibition (18.66±1.154) against E. coli ATCC 25922 except for Bacillus spp in which peel extract showed maximum zone of inhibition i.e. (15±1) Conclusion:This study suggests that these juice and peel extracts may have benefi cial antibacterial roles that can be exploited in controlling unwanted bacterial growth.

Assessment of phenolic content, pesticide residue and biological activities of organic lemon and cucumber in Lebanese market

“…In alignment with our study, Kadhim Hindi and Ghani Chabuck (2013) revealed that lemon juice showed the high inhibition zone of (28 mm) against Enterococcus faecalis bacterial strain (Kadhim Hindi and Ghani Chabuck, 2013). Also, a similar finding was shown by Alisha Shakya et al (2019) who reported an inhibition zone of 20.66 mm against Pseudomonas aeruginosa in lemon samples (Shakya et al, 2019). Additionally, our finding revealed that grampositive bacterial strains exerted better inhibition zone than gram negative in both farming systems.…”

“…The reason behind this is that gram-negative bacteria possess extra outer membrane, which might be able to behave as a barrier for the passage of this polyphenolic compounds. Similarly, other study showed resistance of gram negative against lemon peels due to such alterations in structures between gram positive and gram negative (Shakya et al, 2019).…”

“…By contrast, Sotiroudis et al (2010) showed antibacterial effect for cucumber against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa ( et al, 2010). Such differences might be due to the high genetic diversity of cucumber species and the difference in the isolated strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa chosen for study, as diverse species have their own susceptibility against various antibacterial substances (Shakya et al, 2019).…”

Evaluation of in vivo wound healing activity of Moroccan Citrus reticulata peel extract

“…In the present study, C. reticulata peel extract revealed good antibacterial activity against various pathogenic bacteria, similar to that noticed in a number of studies [51,52] which seemed to have beneficial effects on wound healing.…”

Effectiveness of green betel leaf and lime extract against Staphylococcus aureus and Escherichia coli

“…The essential oil was isolated from the peels (Edogbanya et al 2019) and also found in its leaves (Al-Aamri et al 2018;Lemes et al 2018). The peels are also effective against E. coli (Shakya et al 2019). The peel extract which can inhibit the growth of S. aureus in the sensitive category is 80% (Ekawati et al 2019).…”