Comparative Study of Cellular Morphology of Bone Marrow Aspiration, Imprint Smear Cytology and Trephine Biopsy in Evaluation of Hematological Disorders

Abstract

BACKGROUNDHematological disorders encompass a broad spectrum of disorders affecting blood & bone marrow. Among a myriad of diagnostic tests that can be applied to the analysis of hematological diseases, bone marrow examination is one of the most valuable diagnostic tools. The Bone marrow aspirate and imprint & trephine biopsy specimens are complementary and when both are obtained, provide a comprehensive evaluation of the bone marrow. AIMS/PURPOSE To compare the cellular morphology of Bone marrow aspiration and imprint smears for diagnosis of hematological disorders and compare with biopsy which is taken as gold standard. METHODS A total number of 60 cases in whom bone marrow examination was indicated were evaluated by Bone marrow aspiration (BMA), bone marrow imprint (BMI) and bone marrow biopsy (BMBx). The diagnosis of BMI correlated with t RESULTS he diagnosis of BMBx in 92% cases, which was higher than the value observed with BMA smears (75%). The spreading quality was better and cytological details were better appreciated in BMI as compared to BMA. All of these ndings were reected in the higher diagnostic accuracy of BMI than BMA. CONCLUSION Bone marrow aspiration and biopsy are important tools for evaluating haematological disorders. Bone marrow imprint should be a standard practice in bone marrow sampling procedure