Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex

Xian, Hong; Wang, Ping; Hua, Jingyi; Cheng, Deng-fang; Feng, Jing; Song, Shuhui; Zhang, Li

doi:10.1016/j.ijbiomac.2018.04.177

Cited by 24 publications

(16 citation statements)

References 28 publications

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“…It was also likely that ferric ions interacted with the residues of chains and led to change of molar ratio of monosaccharide. [19] It could be seen from Figure 5a, the boundary of GLP80 was blurred and in an amorphous state, which was consistent with XRD. In Figure 5c, the slightly lighter gray area was GLP80, the black area with clear boundary lattice were GLP80-Fe and the diameter was 50 nm.…”

Section: Analysis Of Monosaccharide Composition By Gcsupporting

confidence: 77%

Structural characteristics and antioxidant activity of polysaccharide-iron complex from Glehniae Radix

Jing

Zhang

et al. 2020

International Journal of Food Properties

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The preparation conditions of Glehniae Radix polysaccharide-iron complex (GLP80-Fe) were optimized and the physicochemical properties were characterized by Ultraviolet-visible spectroscopy (UV-Vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscope (TEM), Thermogravimetric analyzer, and Differential scanning calorimetry (TGA-DSC), respectively. The stability and reducibility of GLP80 and GLP80-Fe were analyzed, and the monosaccharide composition was determined by gas chromatograph (GC). In vitro antioxidant activity of GLP80 and GLP80-Fe were evaluated by determining the radical (DPPH and hydroxyl radical) scavenging abilities. The results showed that the highest iron content of GLP80-Fe (15.61% ±0.04) was obtained at the optimum conditions: reaction temperature was 38.0°C, reaction time was 3.0 h, FeCl 3 addition was 2.9 mL and pH was 9. GLP80-Fe was a complex with β-FeOOH as the core and polysaccharides on its surface, and it was stable at pH 3 ~ 14. GC showed that the major monosaccharides were mannose (Man) and galactose (Gal) with a ratio of 1:5.15. In vitro freeradical scavenging activity of GLP80-Fe was significantly better than GLP80. The IC 50 of GLP80-Fe against DPPH and hydroxyl radical were 3.785 mg/mL and 1.460 mg/mL, respectively.

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Section: Analysis Of Monosaccharide Composition By Gcsupporting

confidence: 77%

Structural characteristics and antioxidant activity of polysaccharide-iron complex from Glehniae Radix

Jing

Zhang

et al. 2020

International Journal of Food Properties

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“…Copper (II) complexes and their ligands also have the ability to scavenge DPPH radicals (Esmaeili et al, 2019). Sulfated polysaccharide iron (III) complex (SPIC) showed a strong effect of scavenging •OH in vitro (Shi et al, 2013;Xian et al, 2018). Wulangerile et al (2016) reported that scavenging effects on hydroxy radical of Narenmandula polysaccharide calcium was between 0.125 and 2.0 mg/mL, which was higher than ulvancalcium in this present study.…”

Section: Discussionsupporting

confidence: 47%

“…According to the literature, basically, all the ulvans were mainly composed of Rha and GlcA, contents of Xyl and Glc vary, while the content of Gal was small. In this study, calcium substituted some active groups or interacted with the hydroxyl of ulvan, which changed the monosaccharide content and caused the variation of molar ratio of monosaccharide (Xian et al, 2018;Zhang et al, 2013).…”

Section: Abts Radical-scavenging Analysismentioning

confidence: 89%

The study on antioxidant activity of ulvan-calcium derivative

Wang¹,

Wan²,

Lin³

et al. 2021

J. Med. Plants Res.

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Ulvan is a water-soluble polysaccharide that is extracted from the algae Ulva pertusa (Chlorophyta). In this study, ulvan-calcium derivative (U-calcium) was prepared and calcium content was 5.2%. Ulvancalcium could effectively scavenge on 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazolie-6-sulfonic acid) (ABTS), hydroxyl, and superoxide radicals. The inhibition rate on superoxide radicals (•O 2-) was 83.14% at 0.2 mg/mL, and the scavenging activity of ABTS and hydroxyl radical (•OH) at 3.0 mg/mL was 58.45 and 43.65%, and that of DPPH radicals at 8.0 mg/mL was 57.37%. All results showed that the antioxidant activity of ulvan-calcium was stronger than that of ulvan. The ulvan-calcium maybe used as an antioxidant to remove ROS and resist excessive oxidative stress.

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“…As shown in Fig. 1A, THP-1 cells treated with PMA at concentrations >25 ng/ml exhibited cell adhesion, spreading and formed pseudopodia, suggesting that the cells successfully differentiated into macrophages (11,12). Furthermore, the level of CD11b, a macrophage surface marker (13), was analyzed in the THP-1 macrophages.…”

Section: Resultsmentioning

confidence: 83%

Total saponin of Dioscorea�collettii attenuates MSU crystal‑induced inflammation via inhibiting the activation of the NALP3 inflammasome and caspase‑1 in THP‑1 macrophages

et al. 2020

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Total saponins extracted from Dioscorea collettii (TSd), extracts of the chinese herb Dioscorea, are thought to exhibit therapeutic benefit in gouty arthritis. However, its exact mechanism remains unclear. The current study aimed to elucidate the underlying mechanisms by investigating the effects of TSD on the inflammation induced by monosodium urate (MSU) crystals in THP-1 macrophages. The viability of THP-1 macrophages was examined using the MTT assay and the levels of inflammatory cytokines, including interleukin (IL)-1β, il-18 and tumor necrosis factor (TnF)-α, released by the cells were quantitatively measured using ELISA kits. The results revealed that the protein level of cluster of differentiation 11b increased in THP-1 cells treated with 100 ng/ml phorbol ester, suggesting that monocytic THP-1 cells were successfully differentiated into macrophages. TSD decreased the levels of inflammatory cytokines, including TnF-α, il-18 and il-1β, secreted by THP-1 macrophages. As the release of IL-1β and il-18 is dependent on the NLR family pyrin domain containing 3 (NALP3) inflammasome and caspase-1, the current study investigated the effect of TSD on the aforementioned proteins. The results revealed that TSD decreased the protein levels of NALP3 and apoptosis-associated speck-like, which serve important roles in the assembly of the NALP3 inflammasome. Furthermore, NALP3 inflammasome-related proteins were also decreased by TSD in rotenone induced THP-1 macrophages, TSD inhibited the activation of caspase-1 and rotenone-induced NALP3 inflammasome activation in THP-1 macrophages. The results obtained in the current study revealed that TSD attenuated MSU crystal-induced inflammation by inhibiting rotenone-induced activation of the NALP3 inflammasome and caspase-1, suggesting that these two proteins may be novel targets for the treatment of gouty arthritis.

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Comparative study of components and anti-oxidative effects between sulfated polysaccharide and its iron complex

Cited by 24 publications

References 28 publications

Structural characteristics and antioxidant activity of polysaccharide-iron complex from Glehniae Radix

Structural characteristics and antioxidant activity of polysaccharide-iron complex from Glehniae Radix

The study on antioxidant activity of ulvan-calcium derivative

Total saponin of Dioscorea�collettii attenuates MSU crystal‑induced inflammation via inhibiting the activation of the NALP3 inflammasome and caspase‑1 in THP‑1 macrophages

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