Comparative study of diagnostic accuracy of established PCR assays and in-house developed sdaA PCR method for detection of Mycobacterium tuberculosis in symptomatic patients with pulmonary tuberculosis

Nimesh, Manoj; Joon, Deepali; Pathak, A. K.; Saluja, Daman

doi:10.1016/j.jinf.2013.06.015

Cited by 16 publications

(20 citation statements)

References 31 publications

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“…In this study, culture and RT-PCR detected a higher number of confirmed TB cases in comparison with microscopy, as was also found by Sethi et al (2012) and Nimesh et al (2013). Moreover, RT-PCR showed high specificity, as found by Broccolo et al (2003) and Armand et al (2011).…”

Section: Discussionsupporting

confidence: 87%

“…None of the four samples with NTM isolation was positive for mpt64, showing the specificity of this target to MTBC detection, as reported by Takahashi & Nakayama (2006), Kusum et al (2012), Sethi et al (2012) and Nimesh et al (2013). All of the patients infected with NTM were notified and treated for TB.…”

Section: Discussionsupporting

confidence: 72%

“…Many regions of the mycobacterial genome have been used in molecular diagnosis of TB, such as IS6110, 16S rRNA, hsp65, rpoB, sdaA, devR and mpt64 (Manjunath et al, 1991;Lachnik et al, 2002;Kim et al, 2004;Chakravorty et al, 2005;Negi et al, 2007;Hwang et al, 2013;Nimesh et al, 2013). mpt64 is found as a single copy in the genome of MTBC species (Lee et al, 1994), and has been used for both pulmonary and extrapulmonary TB diagnosis (Tan et al, 1995;Dar et al, 1998;Martins et al, 2000;Bhanu et al, 2005;Therese et al, 2005;Takahashi & Nakayama, 2006;Dil-Afroze et al, 2008;Kusum et al, 2012;Sethi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Pinhata

Cergole-Novella²,

Carmo³

et al. 2015

Journal of Medical Microbiology

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Tuberculosis (TB) is an infectious disease of global distribution, constituting a serious public health problem in Brazil. Sã o Paulo State, located in the south-east of Brazil, notified 16 580 new TB cases in 2013. The Instituto Adolfo Lutz is a public health reference laboratory for TB diagnosis for all the State. Considering that rapid and accurate diagnosis is essential for TB control, the aim of this study was to evaluate the use of an in-house real-time (RT)-PCR assay targeting the mpt64 gene in the routine diagnosis of TB, and to compare this technique with smear microscopy and culture. From August 2012 to October 2013, 715 sputum samples from 657 patients were included in the study. Smear microscopy, culture, phenotypic and PRAhsp65 identification of mycobacteria, and mpt64 RT-PCR were performed. With respect to confirmed TB cases (n562/657; 9.4 %), smear microscopy had a sensitivity of 82.3 %. Culture and RT-PCR showed the same sensitivity, i.e. 90.3 %. Specificity was 99.7, 99.4 and 98.6 % for smear microscopy, culture and RT-PCR, respectively. mpt64 RT-PCR showed high sensitivity and specificity for the detection of Mycobacterium tuberculosis complex in sputum samples. This technique can be deployed in laboratories that do not have a rapid test for TB available, enabling the performance of TB diagnosis in up to 5 h.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

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Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Pinhata

Cergole-Novella²,

Carmo³

et al. 2015

Journal of Medical Microbiology

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“…This region offers excellent diagnostic sensitivity as a PCR target based on previous evaluation (8). The LAMP primer sequences, with the italicized letters indicating poly(T) linkers in the inner primers, are F3 (5=-TAGGGAAGGGCAACTGAGCA-3=), B3 (5=-AGCGT GATATCGACCTGCAT-3=), FIP (5=-CACGGAACAGACCA GCGG TTTT GGATGTTGGCCGCTGTTG-3=), and BIP (5=-CC GCGGCAGTGAACGTC TTTT GCCAACGCATCCCAACG-3=) (D. Saluja, M. Nimesh, and D. Joon, 10 January 2013, Indian Patent Office, patent application no.…”

mentioning

confidence: 99%

“…The isolated DNA was stored at Ϫ20°C and used for LAMP and PCR assays. sdaA PCR was carried out according to a published protocol (8).…”

mentioning

confidence: 99%

Development and Clinical Evaluation of sdaA Loop-Mediated Isothermal Amplification Assay for Detection of Mycobacterium tuberculosis with an Approach To Prevent Carryover Contamination

et al. 2014

Self Cite

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A rapid and sensitive loop-mediated isothermal amplification assay for the sdaA gene of Mycobacterium tuberculosis was developed using a dUTP-uracil-N-glycosylase (dUTP-UNG) strategy to prevent carryover contamination. Evaluation of the assay using clinical specimens (n ‫؍‬ 648) showed high specificity (97.2%) and sensitivity (100%), demonstrating its potential as a diagnostic test for tuberculosis, especially in resource-limited settings.

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Truenat MTB assays for pulmonary tuberculosis and rifampicin resistance in adults

Inbaraj

Daniel

Rajendran

et al. 2023

Cochrane Database of Systematic Reviews

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Comparative study of diagnostic accuracy of established PCR assays and in-house developed sdaA PCR method for detection of Mycobacterium tuberculosis in symptomatic patients with pulmonary tuberculosis

Cited by 16 publications

References 31 publications

Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Rapid detection of Mycobacterium tuberculosis complex by real-time PCR in sputum samples and its use in the routine diagnosis in a reference laboratory

Development and Clinical Evaluation of sdaA Loop-Mediated Isothermal Amplification Assay for Detection of Mycobacterium tuberculosis with an Approach To Prevent Carryover Contamination

Truenat MTB assays for pulmonary tuberculosis and rifampicin resistance in adults

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