Comparative Study of DTMUV and LPS on Duck Liver Disease

Abstract

Background In order to study the structure of adult duck liver and compare the pathological changes induced by duck Tembusu virus (DTMUV) and lipopolysaccharide (LPS) in duck liver, the structural characteristics of duck liver were identified by HE staining, reticular fiber staining, trichromatic staining, and then the pathological changes caused by DTMUV virus strains XZ-2012 and LPS in adult duck liver were studied from the perspective of comparing viral and bacterial infections. Results The results showed that normal duck liver was reddish brown, the liver lobule boundary was not obvious, and no obvious interlobular bile duct and bile duct were found. By injecting ink into the jugular vein, the location of Kupffer's cells can be located. After DTMUV infection, the liver was swollen, congested, and yellow in color. Lymphocyte infiltration, increased reticular fibers, thickened basement membrane, obvious vacuoles in the liver cells, and increased number of red blood cells in the hepatic sinuses can be seen in the central vein and around the portal area. After LPS infection, the liver was slightly enlarged, no vacuolar degeneration was found in the liver cells, a large number of lymphocytes gathered around the blood vessels, and reticular fibers were significantly increased. The number of Kupffer cells increased after infection. Conclusions The pathological changes induced by DTMUV and lipopolysaccharide after invading the liver were explored, which provide theoretical basis for the pathogenicity of avian virus and bacteria and the defense mechanism of the liver.