Comparative Study of Growth Statistics of Two Species of Paulownia and Optimization of Rooting Methods

Saiju, H. K.; Bajracharya, Abhishesh; Rajbahak, Brishav; Ghimire, Stuti

doi:10.3126/njb.v6i1.22330

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…Rooting experiment was carried out in this study using two auxins combined, as well as some of the treatments separately with other literature such as Shiragave [71] who reported that the efficiency of IBA than other auxins in rooting in Phyllanthus reticulatus. Also, Paulownia tomentosa and Paulownia fortuneii had the longest root and density when Saiju et al [72] employed MS medium with NAA at (0.5 or 1.5 mg/l) in the growing medium. Furthermore, the number of almond peach rootstock hybrids has grown on MS medium containing IBA [73].…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Nower

2022

Journal of Genetic Engineering and Biotechnology

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Background Dimocarpus longan is a tropical tree that produces edible fruit. It is a neglected plant species that is listed as near threatened. In spite of its economic value, the propagation of longan cultivar using conventional methods is extremely difficult. The goal of this research is to produce and conserve this plant through in vitro propagation. Results In order to form new shoots, sterilized shoot tip explants were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) or 2-isobentenyl-adenine (2ip). For direct organogenesis, young leaves of new shoots were cultured on MS medium fortified with various concentrations of Thidiazuron (TDZ) or 6-(4-Hydroxy-3-methylbut-2-enylamino purine) (Zeatin). Gibbrellic acid (GA3) at different levels alone or in combination was used for shoot elongation. Also, indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) were used for root formation. MS medium supplemented with 1.00 mg/l 2ip was suitable for inducing axillary shoots from shoot tips (4.0 axillary shoots/explant). The highest significant 76% and numbers of adventitious buds from leaf base were achieved on MS medium containing 1.0 mg/l TDZ. These buds developed into the longest plantlets on GA3 at 3.0 mg/l and rooted well in ½MS containing 1.50 mg/l IBA plus 0.50 mg/l (NAA). About 70% in vitro plants were successfully acclimatized. The AFLP profile illustrated the genetic stability of gene expression action. The amplified fragment length polymorphisms (AFLPs) profile illustrated the progenies were extremely similar to the mother plants. According to our findings, MS medium containing 25 ppm salicylic acid (SA) and 5 ppm methyl jasmonate (MeJA) produced the highest percentage of apigenin in longan calli (77.09 and 2.637%, w/w). Conclusion A successful and efficient micropropagation protocol has been developed and described here for the first time, and it will be very useful for the clonal propagation and conservation of the near-threatened Dimocarpus longan plant. Micropropagated plants are genetically identical to the donor plant using the AFLP technique. The usefulness of salicylic acid and methyl jasmonate as elicitors for increasing in vitro production of secondary metabolites in plants is demonstrated in this work.

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Section: Discussionmentioning

confidence: 99%

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

Nower

2022

Journal of Genetic Engineering and Biotechnology

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In vitro study on the effect of cytokines and auxins addition to growth medium on the micropropagation and rooting of Paulownia species (Paulownia hybridandPaulownia tomentosa)

Mohamad

Awad

Majrashi

et al. 2022

Saudi Journal of Biological Sciences

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Cultivation Potential and Uses of Paulownia Wood: A Review

Jakubowski¹

2022

Forests

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This review aimed to determine the current state of research on the growth conditions and use pertaining to paulownia wood, mainly in European countries where paulownia has been introduced only relatively recently. Several studies carried out on Paulownia hybrids have shown significant differences in the growth dynamics of individual clones in their response to local environmental and climatic conditions. For example, dry biomass production yields in the second year of cultivation range from 1.5 t ha−1 to as much as 14 t ha−1. This diversity has manifested itself not only in growth characteristics but also in the properties of the wood and the possibilities for its use. Despite having clear similarities to the genus Paulownia, the cultivation of species and hybrids under different conditions has produced varying results. The best growing conditions for this wood (that make economic sense) are in the Middle East and Southern Europe. These regions have accumulated the most experience because of the earlier establishment of the crop. Today, paulownia cultivation is dominated by hybrids with selected traits that are propagated mainly in vitro. The most commonly planted hybrids include the clones in vitro 112, Cotevisa 2 and Shan Tong. The growth results and production capacity in central European countries are lower compared to Southern Europe. Experiments on paulownia cultivation are still relatively young, mainly consisting of replicating the cultivation of hybrids developed in Asia or Southern Europe. However, agronomic procedures are being developed and reactions to local climatic conditions are being studied. It is likely that, in the next few years, the profitability of growing paulownia in these regions will become apparent.

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Comparative Study of Growth Statistics of Two Species of Paulownia and Optimization of Rooting Methods

Cited by 3 publications

References 8 publications

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

In vitro propagation for conservation and genetic fidelity of the near threatened Dimocarpus longan plant

In vitro study on the effect of cytokines and auxins addition to growth medium on the micropropagation and rooting of Paulownia species (Paulownia hybridandPaulownia tomentosa)

Cultivation Potential and Uses of Paulownia Wood: A Review

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