Comparative study of mast cell count in oral reactive lesions and its association with inflammation

Abstract

Objective: Reactive hyperplastic lesions are one of the most common lesions of the oral cavity. Mast cells can be found in various oral lesions. however, the exact role of mast cells in oral reactive lesions is not fully understood. The aim of this study was to compare the mast cells count in various oral reactive lesions and to evaluate the correlation between the mast cells number and the intensity of inflammation. Material and Method:This cross-sectional study was performed on 80 samples of oral reactive lesions including irritation fibroma, pyogenic granuloma, peripheral giant cell granuloma and peripheral ossifying fibroma (20 for each). eight samples of normal oral mucosa were selected as the control group. To determine the mast cells count and the intensity of inflammation, Toluidine blue and hematoxylin & eosin staining was performed respectively. The data was analyzed with SPSS (V.21), Kruskal-Wallis, Tukey and Spearman's correlation coefficient tests. Results:In this study, a significant increase in mast cells count was found in oral reactive lesions compared to normal oral mucosa (p<0.001). Difference of mast cell count between irritation fibroma and the peripheral giant cell granuloma groups (p=0.023), peripheral ossifying fibroma and peripheral giant cell granuloma groups (p=0.001) was statistically significant. There was a significant correlation between mast cell count and the intensity of inflammation in the irritation fibroma and pyogenic granuloma groups. Conclusion:The finding of this study showed that mast cells count in peripheral ossifying fibroma and irritation fibroma were significantly higher than normal oral mucosa, so mast cells may have some role in the induction of fibrosis in oral reactive lesions.

Quantification of inflammatory, angiogenic, and fibrous components of reactive oral lesions with an insight into the pathogenesis

“…Chymase and tryptase from mast cells facilitate fibroblast proliferation and type I collagen synthesis. [ 24 ] According to our findings, the severity of inflammation was high in pyogenic granulomas, followed by inflammatory fibrous hyperplasia and irritation fibroma. Hunasgi et al .…”

“…The fibrotic events may be attributed to the functional fibroblasts activation by fibrogenic cytokines such as tumour necrosis factor, platelet-derived growth factor, and basic fibroblast growth factor. [ 24 30 ] Epithelial thickness was increased in inflammatory fibrous hyperplasia compared to other lesions. This may be due to the cytokines released by the inflammatory cells, resulting in the acanthosis and pseudoepitheliomatous hyperplasia of the overlying epithelium.…”

A large-cohort study of 2971 cases of epulis: focusing on risk factors associated with recurrence

“…The low recurrence rate of PGCG might be partly explained by low capacity in neovascularization of granulation tissue. Another reason was that the mast cell count of PGCG was the lowest among all types of epulis [ 10 , 31 ], while the mast cells were important sources of some proangiogenic and angiogenic factors [ 32 ]. Striking difference was reported in the results by Babu and Savage [ 7 , 13 ], which demonstrated that PGCG had the highest recurrence rate among all types of epulis because the cells of PGCG show high proliferative potential.…”

Pleomorphic adenoma of the cheek: a case presentation

“…Soft-tissue neoplasms, like neurilemoma [8,10] neurofibroma, lipoma [8,14] neoplasms of minor salivary glands, may also be considered for the differential diagnosis [18], in addition to inflammatory and reactive lesions [8][9][10].…”