Comparative study of morphological, cultural and molecular markers for the characterization ofPseudocercosporella herpotrichoides isolates

Gac, M. L.; Montfort, Françoise; Cavelier, N.; Sailland, A.

doi:10.1007/bf01878127

Cited by 13 publications

(8 citation statements)

References 31 publications

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“…In particular, such assays reduce the pathologist's reliance on culturing pathogens out of plant tissue. PCR-based detection methods have been developed for many species, including F. avenaceum, F. culmorum and F. graminearum (Schilling et al, 1996), F. poae , Tapesia acuformis and Tapesia yallundae (Gac et al, 1996;Nicholson et al, 1997), Microdochium nivale and Rhizoctonia cerealis . Though the importance of PCR-based pathogen detection tools is widely recognized, this study shows that caution is required in their design and use.…”

Section: A S Turner Et Al 286mentioning

confidence: 99%

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Lees²,

et al. 1998

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Existing PCR-based assays for the detection of the cereal pathogen Fusarium avenaceum were found to cross-react with F. tricinctum. An investigation into the phenetic relationship between these two species using two different marker systems revealed a close relationship between them despite their being from separate taxonomic sections. Whilst RFLP differences in the ITS regions surrounding the nuclear 5·8 S rDNA were insufficient to discriminate between isolates of the two species, they were clearly differentiated by RAPD profiling. RAPD fragments from F. avenaceum isolates were screened for hybridization to F. tricinctum DNA on Southern blots. One of 12 selected RAPD fragments showed no hybridization to genomic DNA from F. tricinctum. This fragment was cloned and sequenced, and the sequence obtained was used to design PCR primers. The primers were found to be specific for F. avenaceum, with no cross-reactions obtained with F. tricinctum or any other wheat pathogen assayed. The primers were able to differentiate between the two species in infected plant material, in contrast to the earlier assays.

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Section: A S Turner Et Al 286mentioning

confidence: 99%

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Lees²,

et al. 1998

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“…RFLP analysis of the ITS and intergenic spacer (IGS) regions of ribosomal DNA have also been used to detect genetic diversity in fungi (e.g. Woudt et al, 1995 ;Gac et al, 1996). The use of more than one type of molecular marker is able to provide supportive and complementary evidence in studies of genetic variability (Edel et al, 1995 ;Arora, Hirsch & Kerry, 1996).…”

Section: Genetic Diversity Of Microdochium Nivale Isolates From Turfgmentioning

confidence: 99%

Genetic diversity of Microdochium nivale isolates from turfgrass

Mahuku¹,

Hsiang

Yang

1998

Mycological Research

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“…The rDNA genes were used to design PCR‐based tests to rapidly differentiate the two types of Oculimacula isolates (Poupard et al. 1993; Gac et al. 1996) that were not definitively known as separate species at that time.…”

Section: Molecular Genetic Studies Of the Pathogensmentioning

confidence: 99%

Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

Wei

Muranty

Zhang

2011

Journal of Phytopathology

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Eyespot disease caused by the soil-borne facultative fungi Oculimacula yallundae and O. acuformis is the major component of the stem-base disease complex of wheat in temperate regions of the world with a cool and wet climate. In this review, we focus on results of genetic studies concerning both partners of the host-pathogen interaction. This comprises analyses of genetic diversity of the pathogen and identification of particular genes within it, evaluation and screening methods for host resistance, resistance sources and genetics of these resistances, breeding of resistant cultivars in wheat, and application of genetic markers in tagging and tracking of eyespot resistance genes. We also attempt to foresee some of the key issues and developments that may occur in future. The identification of markers tightly linked to eyespot resistance genes is the important research focus opening the door to marker-assisted selection of resistant varieties.

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Comparative study of morphological, cultural and molecular markers for the characterization ofPseudocercosporella herpotrichoides isolates

Cited by 13 publications

References 31 publications

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Refinement of PCR‐detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum

Genetic diversity of Microdochium nivale isolates from turfgrass

Advances and Prospects in Wheat Eyespot Research: Contributions from Genetics and Molecular Tools

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