Comparative Study of New Hplc And Hptlc Methods For Simultaneous Determination of Dipyrone And Camylofin Dihydrochloride Using Hyoscine Butyl Bromide As Internal Standard

Atta, A M; Gomaa, Mohamed S.; Salama, Ismail; Mostafa, Samia M.

doi:10.9790/3008-1104043646

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…It is found in market as single or combined formulation with paracetamol, nimesulide, diclofenac and analgin. The literature revealed many analytical techniques for the quantification of camylofin-2HCl such as spectrophotometry [3,4], HPLC [5][6][7][8][9] TLC densitometry [6,7,[10][11][12] and GC [13,14].…”

Section: Introductionmentioning

confidence: 99%

Validated HPLC and stability-indicating densitometric chromatographic methods for simultaneous determination of camylofin dihydrochloride and paracetamol in their binary mixture

2020

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Two accurate, sensitive, precise and selective HPLC and stability-indicating TLC methods were developed for the simultaneous determination of camylofin-2HCl and paracetamol. Forced acid, alkali and oxidative degradation of camylofin-2HCl were tried where complete degradation was achieved using 5 N HCl. HPLC method was developed to determine the mixture of the two drugs using Zorbax NH 2 column and a mobile phase of 0.5% triethylamine and pH 3.0 adjusted with 0.1% phosphoric acid and methanol (70:30 v/v) over concentration ranges of 3-90 and 10-95 µg/mL for camylofin-2HCl and paracetamol, respectively.TLC method was used for the separation of camylofin from its acid degradate and paracetamol using chloroform-methanol-acetone-conc. ammonia (8:2:2:0.1, by volume) as developing system and band scanning at 254 nm over concentration ranges of 5-40 µg/band for camylofin-2HCl and 0.1-0.5 µg/band for paracetamol. The validation of two methods was carried out according to ICH guideline. Accuracy ranged between 98.47 and 100.67% for the two methods with acceptable precision RSD% ranging between 0.66 and 1.47%.

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Section: Introductionmentioning

confidence: 99%

Validated HPLC and stability-indicating densitometric chromatographic methods for simultaneous determination of camylofin dihydrochloride and paracetamol in their binary mixture

2020

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“Eco-friendly HPLC method for analysis of dipyrone and hyoscine in different matrices with biomonitoring”

El kalla,

Ghoniem,

Zaazaa

et al. 2024

Sci Rep

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A selective, precise, and accurate reversed HPLC method has been developed and validated for simultaneous separation and determination of two veterinary drugs, dipyrone and hyoscine, in their combined dosage form in the presence of their official impurities, namely 4-aminoantipyrine and tropic acid, in addition to the formulated preservative: phenol. The linearity range was found to be (1.00–35.00 µg/mL) for dipyrone and (2.50–50.00 µg/mL) for hyoscine. It exhibited a satisfactory linearity regression R (0.9999) for both drugs with LOD 0.22 µg/mL and 0.72 µg/mL and LOQ 0.65 µg/mL and 2.19 µg/mL for dipyrone and hyoscine, respectively. Additionally, the two cited drugs were also determined in the presence of dipyrone active metabolite 4-aminoantipyrine using diclofenac as an internal standard in bovine urine. The linearity range was found to be (15–75 µg/mL) for dipyrone, (2.5–60 µg/mL) for hyoscine, and (2.5–60 µg/mL) for 4-aminoantipyrine with linearity regression R (0.9999–0.9998). The LLOQ (15, 2.5, 2.5 µg/mL), LQC (45, 7.5, 7.5 µg/mL), MQC (55, 25, 25 µg/mL), and HQC (60, 50 50 µg/mL) were determined for dipyrone, hyoscine and 4-aminoantipyrine, respectively. UV detection was carried out at 220 nm. The method was validated according to the ICH guidelines, as well as according to FDA guidelines for determining both drugs in bioanalytical matrices and both proved accuracy and precision. A statistical comparison was made between the results obtained and those obtained by the reported method, showing no significant difference in accuracy and precision at p = 0.05. The suggested method was proved eco-friendly through a greenness assessment using two different tools (The analytical eco-scale scored 83, and the AGREE-Analytical Greenness Metric approach scored 0.83). The suggested method can be used in the routine work of quality control labs, screening for drug abuse, and ensuring clean sport for horse racing.

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Comparative Study of New Hplc And Hptlc Methods For Simultaneous Determination of Dipyrone And Camylofin Dihydrochloride Using Hyoscine Butyl Bromide As Internal Standard

Abstract: 60 F 254 using acetone: methanol: acetic acid (52: 28: 20, v/v/v)

Cited by 2 publications

References 11 publications

Validated HPLC and stability-indicating densitometric chromatographic methods for simultaneous determination of camylofin dihydrochloride and paracetamol in their binary mixture

Validated HPLC and stability-indicating densitometric chromatographic methods for simultaneous determination of camylofin dihydrochloride and paracetamol in their binary mixture

“Eco-friendly HPLC method for analysis of dipyrone and hyoscine in different matrices with biomonitoring”

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