Biological treatment is one of the best methods used to treat wastewater because it is environmentally friendly and costs less than chemical treatment methods. Experimental work was conducted to treat aquaculture wastewater and reuse it using white aggregate and gravel mechanical filter media and rice straw, activated carbon, plastic beads and banana peel as biofilter media. Factors under investigation were filter media type, ammonia (NH 3 ) concentration, HRT, measurement indicators were pH, TDS, TSS, NTU, COD, BOD, TKN. The results showed that gravel mechanical filter is better than white aggregate mechanical filter in reducing NH 3 concentration and physico-chemical parameters. NH 3 concentration in raw wastewater was 32 mg/l and reduced to 28, 25 mg/l in white aggregate and gravel mechanical filter, respectively. For gravel mechanical filter, NH 3 concentration after passing through activated carbon, rice straw, plastic beads and banana peel was 5, 9.72, 10.8 and 5.5 mg/l, respectively. Four hydraulic retention time and doses of activated carbon per liter of wastewater were used, 5, 10, 15 and 20 gm and 5, 10, 20 and 30 min. 20 min with a medium dose of 15 gm of activated carbon per liter of wastewater had the best effect on NH 3 removal. Five hydraulic retention time and doses of rice straw per liter of wastewater were used, 5, 10, 15, 20 and 25 gm and 5, 10, 20, 30 and 40 min. 30 min with a medium dose of 20 gm of rice straw per liter of wastewater had the best effect on NH 3 removal.