Comparative study of pathological lesions induced by multiwalled carbon nanotubes in lungs of mice by intratracheal instillation and inhalation

Li, Jungang; Li, Wenxin; Xu, Jingying; Cai, Xiaoqing; Liu, Ruili; Li, Yongjun; Zhao, Qing; Li, Qingnuan

doi:10.1002/tox.20270

Cited by 168 publications

(91 citation statements)

References 16 publications

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“…Results indicate that inhalation exposure resulted in 4-fold greater inflammation and fibrosis than aspiration of the same mass lung burden. Li et al 47 reported similar findings, that is, inhalation of a more dispersed MWCNT preparation caused greater interstitial fibrosis and cell proliferation than aspiration of a less dispersed preparation. Mercer et al 15 found that when efforts were made to improve the dispersion of aspirated SWCNT suspensions, fibrotic potency was similar between aspiration and a 4 day inhalation exposure.…”

Section: Relationships Between Bolus Pulmonary Exposure (It or Asmentioning

confidence: 75%

Occupational Nanosafety Considerations for Carbon Nanotubes and Carbon Nanofibers

2012

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CONSPECTUSCarbon nanotubes (CNTs) are carbon atoms arranged in a crystalline graphene lattice with a tubular morphology. CNTs exhibit high tensile strength, possess unique electrical properties, are durable, and can be functionalized. These properties allow applications as structural materials, in electronics, as heating elements, in batteries, in the production of stain-resistant fabric, for bone grafting and dental implants, and for targeted drug delivery. Carbon nanofibers (CNFs) are strong, flexible fibers that are currently used to produce composite materials.Agitation can lead to aerosolized CNTs and CNFs, and peak airborne particulate concentrations are associated with workplace activities such as weighing, transferring, mixing, blending, or sonication. Most airborne CNTs or CNFs found in workplaces are loose agglomerates of micrometer diameter. However, due to their low density, they linger in workplace air for a considerable time, and a large fraction of these structures are respirable.In rat and mouse models, pulmonary exposure to single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), or CNFs causes the following pulmonary reactions: acute pulmonary inflammation and injury, rapid and persistent formation of granulomatous lesions at deposition sites of large CNT agglomerates, and rapid and progressive alveolar interstitial fibrosis at deposition sites of more dispersed CNT or CNF structures.Pulmonary exposure to SWCNTs can induce oxidant stress in aortic tissue and increases plaque formation in an atherosclerotic mouse model. Pulmonary exposure to MWCNTs depresses the ability of coronary arterioles to respond to dilators. These cardiovascular effects may result from neurogenic signals from sensory irritant receptors in the lung. Pulmonary exposure to MWCNTs also upregulates mRNA for inflammatory mediators in selected brain regions, and pulmonary exposure to SWCNTs upregulates the baroreceptor reflex. In addition, pulmonary exposure to MWCNTs may induce levels of inflammatory mediators in the blood, which may affect the cardiovascular system.

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Section: Relationships Between Bolus Pulmonary Exposure (It or Asmentioning

confidence: 75%

Occupational Nanosafety Considerations for Carbon Nanotubes and Carbon Nanofibers

2012

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“…There are few studies that have developed aerosolization systems and delivered carbon-based nanomaterials via inhalation (Li et al 2007;Mitchell et al 2007;Ryman-Rasmussen et al 2008;Shvedova et al 2008). In the Mitchell et al (2007) study, MW-CNTs were aerosolized with a jet mill coupled to a dry chemical screw feeder and a 2-µm cut-point cyclone.…”

Section: Discussionmentioning

confidence: 99%

Aerosolization System for Experimental Inhalation Studies of Carbon-Based Nanomaterials

Madl

Teague²,

et al. 2012

Aerosol Science and Technology

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Assessing the human health risks associated with engineered nanomaterials is challenging because of the wide range of plausible exposure scenarios. While exposure to nanomaterials may occur through a number of pathways, inhalation is likely one of the most significant potential routes of exposure in industrial settings. An aerosolization system was developed to administer carbon nanomaterials from a dry bulk medium into airborne particles for delivery into a nose-only inhalation system. Utilization of a cannula-based feed system, diamond-coated wheel, aerosolization chamber, and krypton-85 source allows for delivery of otherwise difficult to produce respirable-sized particles. The particle size distribution (aerodynamic and actual) and morphology were characterized for different aerosolized carbon-based nanomaterials (e.g., single-walled carbon nanotubes and ultrafine carbon black). Airborne particles represented a range of size and morphological characteristics, all of which were agglomerated particles spanning in actual size from the nanosize range (<0.1 µm) to sizes greater than 5 and 10 µm for the particle's largest dimension. At a mass concentration of 1000 µg/m 3 , the size distribution as measured by the inertial impactor ranged from 1.3 to 1.7 µm with a σ g between 1.2 and 1.4 for all nanomaterial types. Because the aerodynamic size distribution is similar across different particle types, this system offers an opportunity to explore mechanisms by which different nanomaterial physicochemical characteristics impart different health effects while theoretically maintaining comparable deposition patterns in the lungs. This sys-

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“…High percentages of early miscarriages and fetal malformations in female rats also have been reported following exposure to CNTs during pregnancy (Pietroiusti et al, 2011). In spite of adverse effects of CNTs, some investigations have reported that CNTs have no serious effect on various biological systems (Li et al, 2007;Garza et al, 2008;Bai et al, 2010;Wessels et al, 2011). The different outcomes following exposure to CNTs likely related to the types of CNTs, manipulation of CNTs (e.g., functionalizing of CNTs), physiology of exposed organism, posology and method of administration of CNTs.…”

Section: Introductionmentioning

confidence: 99%

Biosafety of multiwalled carbon nanotube in mice: a behavioral toxicological approach

2012

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-Multiwalled carbon nanotubes (MWCNTs) with unique chemical and electromechanical properties are ideal candidates for the development of drug delivery platforms. The scarce knowledge for the effects of exposure to MWCNTs during pregnancy on postnatal outcomes motivated us to investigate whether intraperitoneal injections (i.p.) of MWCNTs during mating and early pregnancy affect on reproductive and neurobehavioral endpoints and psychobiological status of pups. Thirty virgin female mice were divided to three groups (n = 10 for each); two treated groups injected i.p. with 1 and 10 mg of MWCNTs suspended in 1 ml of phosphate buffered saline solution (PBS) in both mating day and gestation day 3, respectively. The control group was injected i.p. with an equal volume of PBS as a vehicle. MWCNT-treated dams did not exhibit considerable changes in their reproductive performance in gestation and lactation periods. MWCNT-treated pups exhibited similar ontogenetic expressions of neurobehavioral and physical endpoints as compared with control group. Most notably, exposure to MWCNTs was increased depressive and anxious behaviors of treated pups in parallel to adverse effect on their internal organ weights. The absolute thymus weight was declined in MWCNT-treated groups while absolute weights of liver and spleen decreased in group treated by 1 mg of MWCNT as compared to control group. Relative organ weights in MWCNT-treated groups were almost similar to control group.

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Comparative study of pathological lesions induced by multiwalled carbon nanotubes in lungs of mice by intratracheal instillation and inhalation

Cited by 168 publications

References 16 publications

Occupational Nanosafety Considerations for Carbon Nanotubes and Carbon Nanofibers

Occupational Nanosafety Considerations for Carbon Nanotubes and Carbon Nanofibers

Aerosolization System for Experimental Inhalation Studies of Carbon-Based Nanomaterials

Biosafety of multiwalled carbon nanotube in mice: a behavioral toxicological approach

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