Comparative study of preimplantation development and embryonic loss in two rabbit strains

Torrès, Suzanne; Hulot, Françoise; Meunier, M.; Sevellec, Claude

doi:10.1051/rnd:19870510

Cited by 12 publications

(12 citation statements)

References 4 publications

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“…The mean value for ovulation rate was within the range of those obtained by other authors in multiparous females selected by reproductive traits (reviewed by Blasco et al, 1993). Fertilization rate, estimated 30 h postmating, was high (99.4%), similar to the results obtained in lines selected for reproductive traits (Torres et al, 1987;García-Ximénez and Vicente, 1992;Bolet and Theau-Clément, 1994;Peiró et al, 2007).…”

Section: Resultssupporting

confidence: 87%

“…Additionally, embryos were graded following the Veeck and Maloney criterion (Veeck and Maloney, 1986) as: Grade 1: embryo with blastomeres of equal size and no cytoplasmic fragments; Grade 2: embryo with blastomeres of equal size and minor cytoplasmic fragments; Grade 3: embryo with blastomeres of distinctly unequal size and none or few cytoplasmic fragments; Grade 4: embryo with blastomeres of equal or unequal size and significant cytoplasmic fragmentation; Grade 5: embryo with few blastomeres of any size and severe or complete fragmentation. Finally, embryos were clustered in embryo-grade categories following the Shulman criterion (Shulman et al, 1993); good quality clustered grade 1 and 2, fair quality clustered grade 3 and poor quality clustered grade 4 and 5.…”

Section: Traits Analysedmentioning

confidence: 99%

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Effect of divergent selection for uterine capacity on embryonic survival and development at 30 h post-mating in unilaterally ovariectomized rabbit females

et al. 2015

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Uterine capacity has been proposed as an indirect way to increase litter size. The aim of this work is to study the effect of a divergent selection for uterine capacity (UC) on reproductive traits at 30 h post mating in unilaterally ovariectomized (ULO) females. A total of 62 ULO females from the high line (selected to increase UC) and 39 ULO females from the low line (selected to decrease UC) were used. Ovulation rate was estimated as the number of corpora haemorrhagica and early embryonic survival was estimated as the ratio between number of embryos and ovulation rate. No differences in ovulation rate and early embryonic survival at 30 h post mating were found between high and low lines. Selection for UC did not change the embryonic stage of development either, the majority of embryos being at 4-cell stage. Additionally, the embryos were evaluated according to morphological criteria and more than 95% of the embryos were evaluated as good or fair quality. No differences in embryonic morphological criteria between high and low lines were found either. Thus, selection for UC did not modify the early embryonic survival and development in ULO females at 30 h post mating.

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Section: Resultssupporting

confidence: 87%

Section: Traits Analysedmentioning

confidence: 99%

Effect of divergent selection for uterine capacity on embryonic survival and development at 30 h post-mating in unilaterally ovariectomized rabbit females

et al. 2015

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“…Fertilisation rate was high, agreeing with results obtained by other authors in rabbits (Torres et al, 1987b;García-Ximénez and Vicente, 1992;Bolet and Theau-Clement, 1994). There is no information on the effect of unilateral ovariectomy on fertilisation rate in rabbits.…”

Section: Discussionsupporting

confidence: 88%

The effect of unilateral ovariectomy on early embryonic survival and embryo development in rabbits

et al. 2014

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Unilateral ovariectomy can be used to study uterine capacity in rabbits because an overcrowding of the functional uterine horn is produced. Due to the uterus duplex, the rabbit is the ideal model for such studies. However, this technique may affect embryo survival. The aim of this work is to study the effect of unilateral ovariectomy on early embryo survival and development in rabbit. A total of 101 unilateral ovariectomised females and 52 intact females were compared after slaughter at 30 h post-mating. Early embryo survival was estimated as the ratio between number of embryo recovered and ovulation rate. No differences were found between intact and unilaterally ovariectomised females in this trait. Unilateral ovariectomy did not change embryo development, measured as the number of embryo cells. Variability of embryo development was not affected either. At 30 h post-mating, the majority of embryos (86.2%) were 4-cell stage. Embryo quality was evaluated according to morphological criteria. No difference in embryo quality between intact and unilaterally ovariectomised females was found. Therefore, unilateral ovariectomy performed before puberty in rabbit does not modify early embryo survival and development.

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“…Oocytes which ovulate first are fertilised earlier and advance the uterine secretions (Torres et al, 1984 in rabbits;Pope, 1988 andXie et al, 1990 in pigs;Wilmut et al, 1986 andAl-Shorepy et al, 1992 in mice). Asynchrony between embryonic development and uterine secretions can cause embryo mortality, as shown in asynchronous embryo transfer experiments in rabbits (Wintenberger- Torres, 1974;Torres et al, 1987). For foetal period comprised from implantation to 18 d of gestation, treated females also had lower survival (D=-0.08 and P=0.93 for FS LF18 ; Table 3).…”

Section: Survival Ratesmentioning

confidence: 88%

Effect of increased ovulation rate on embryo and foetal survival as a model for selection by ovulation rate in rabbits

et al. 2016

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Selection for ovulation rate in prolific species has not improved litter size, due to an increase in prenatal mortality, with most mortality observed in the foetal period. The aim of this study was to investigate the magnitude and timing of embryo and early foetal survival in females with high ovulation rate using hormonal treatment as a model for selection by ovulation rate. Two groups of females (treated and untreated) were used. Treated females were injected with 50 IU equine chorionic gonadotropin 48 h before mating. Females were slaughtered at 18 d of gestation. Ovulation rate (OR), number of implanted embryos (IE), number of live foetuses at 12 and 18 d (LF 12 and LF 18 , respectively) were recorded. In addition, embryo survival (ES=IE/OR), foetal survival at 18 d of gestation (FS LF18 =LF 18 /IE), foetal survival between 12 and 18 d of gestation (FS LF18/LF12 =LF 18 /LF 12 ) and prenatal survival (PS LF18 =LF 18 /OR) were estimated. For each female, the mean and variability of the weight for live foetuses (LFWm and LFWv, respectively) and their placentas (LFPWm and LFPWv, respectively) were calculated. Treated females had a higher ovulation rate (+3.02 ova) than untreated females, with a probability of 0.99. An increase in the differences (D) between treated and untreated females was observed from implantation to 18 d of gestation (D=-0.33, -0.70 and -1.28 for IE, LF 12 and LF 18 , respectively). These differences had a low accuracy and the probability that treated females would have a lower number of foetuses also increased throughout gestation (0.60, 0.70 and 0.86 for IE, LF 12 and LF 18 , respectively). According to the previous results for OR and LF 18 , treated females showed a lower survival rate from ovulation to 18 d of gestation (D=-0.12, P=0.98 for PS LF18 ). Treated females also had lower embryo and foetal survival (D=-0.10 and P=0.94 for ES and D=-0.08 and P=0.93 for FS LF18 ). Main differences in foetal survival appeared from 12 to 18 d of gestation (D=-0.09 and P=0.98 for FS LF18/LF12 ). Unexpectedly, treated females showed similar foetus weight and higher foetal placenta weight than untreated females (D=0.25 g, P=0.98) and lower variability for these traits (D=-0.02 g, P=0.72 for LFWv and D=-0.05 g, P=0.83 for LFPWv). These results are not related to a lower number of IE or LF 18 . Thus, the effect of increasing by three ova in rabbits leads to a lower embryo and early foetal survival. There seems to be no relationship between foetal mortality and foetus weight.

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Comparative study of preimplantation development and embryonic loss in two rabbit strains

Cited by 12 publications

References 4 publications

Effect of divergent selection for uterine capacity on embryonic survival and development at 30 h post-mating in unilaterally ovariectomized rabbit females

Effect of divergent selection for uterine capacity on embryonic survival and development at 30 h post-mating in unilaterally ovariectomized rabbit females

The effect of unilateral ovariectomy on early embryonic survival and embryo development in rabbits

Effect of increased ovulation rate on embryo and foetal survival as a model for selection by ovulation rate in rabbits

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