Comparative Study of Serological Tests for<i>Mycoplasma synoviae</i>Diagnosis in Commercial Poultry Breeders

Luciano, Renato Luis; Cardoso, António J. Marques; Stoppa, Greice Filomena Zanatta; Kanashiro, Ana Maria Iba; Castro, Antonio Guilherme Machado de; Tessari, Eliana Neire Castiglioni

doi:10.4061/2011/304349

Cited by 17 publications

(16 citation statements)

References 18 publications

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“…ELISA is not feasible for sero monitoring because it is more time consuming and costly (Higgins and Whithear, 1986). False positive results can give a very high prevalence by SPA test which are because of cross reactivity, use of inactivated vaccine, contaminated sera and age of the flock (Luciano et al, 2011). Major constraint in the use of SPA test for diagnosis is its low specificity (Pourbakhsh et al, 2010).…”

Section: Serological Techniquesmentioning

confidence: 99%

“…SPA can be used for screening flocks but not for screening individual birds. For proper diagnosis and control, programs based on sero conversion may be inadequate and sero monitoring should be combined with culture and molecular techniques (Luciano et al, 2011).M. gallisepticum is shown to be cross reactive with closely related M. imitans that would also lead to aberrance in prevalence of specific Mycoplasma species (Bradbury et al, 1993).…”

Section: Serological Techniquesmentioning

confidence: 99%

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Mycoplasmosis in Poultry, a Perpetual Problem

Shoaib¹

2019

J microb biotech food sci

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Bacterial diseases are a huge concern for poultry farmers and cause huge economic losses to poultry industry every year. Various diseases can initiate respiratory signs in poultry, including mycoplasmosis. Mycoplasmosis is a major threat currently faced by poultry industry worldwide. In poultry main pathogenic species of Mycoplasma are Mycoplasma gallisepticum and Mycoplasma synoviae. Mycoplasma is a worldwide avian pathogen that causes immense losses in the poultry industry by decreasing eggs production, reducing growth and increased condemnation at slaughter houses. Mycoplasma is a wall-less bacterium that causes chronic respiratory disease in all types of chickens. Mycoplasmosis is an egg transmitted, hatchery disseminated and economically very important disease of chickens. Commonly used methods for diagnosis of Mycoplasma include isolation and identification of bacteria, several serological tests and molecular techniques. Closely located poultry farms, rearing of mixed avian species in close milieus, mixed commercial poultry farming and presence of wild birds in close proximity to poultry farms have made this disease very difficult to control. The review summarized the pathogenesis of Mycoplasma, associated risk factors, diagnostic tools and control measures. Best measures to control Mycoplasmosis include biosecurity, hygiene, good management, monitoring and removal of infected flocks, routine vaccination and farmer awareness. To reduce the risk of transmission of disease to other poultry populations, there should be continued monitoring of flocks for Mycoplasma.

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Section: Serological Techniquesmentioning

confidence: 99%

Section: Serological Techniquesmentioning

confidence: 99%

Mycoplasmosis in Poultry, a Perpetual Problem

Shoaib¹

2019

J microb biotech food sci

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“…Bu durumda parametrik olmayan testlerden McNemar testi yaygın olarak kullanılmaktadır. 9,10 McNemar testi eşleştirilmiş deneklerle yapı-lan deneylerde "evet" ve "hayır" yanıtları gibi iki olası sonucu olan çalışmaları analiz etmek için kullanılan bir yöntemdir. McNemar yöntemi 2*2'lik tablo ile özetlenir.…”

Section: Mcnemar Testi̇unclassified

Practical Approaches For Determination Of Sample Size In Paired Case-Control Studies

Demirel¹,

Oruç²,

Gürler³

2016

Turkiye Klinikleri J Biostat

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Neslihan DEMİREL ve ark.EŞLEŞTİRİLMİŞ DURUM-KONTROL ÇALIŞMALARINDA ÖRNEKLEM GENİŞLİĞİNİN BELİRLENMESİ...Turkiye Klinikleri J Biostat 2016;8(1) 36 ORİJİNAL ARAŞTIRMA ORIGINAL RESEARCH linik araştırmalar genellikle yeni bir ilaç veya tıbbi aletin canlılar-da kullanımının etkin ve güvenilir olup olmadığını göstermek için yapılır. Bu nedenle tıp ve veteriner hekimlik alanlarında fazla sayıda deneysel çalışma yapılmaktadır. Maliyetli ve zaman alıcı olan bu ça-lışmalarda sonuçların güvenilir olabilmesi için istatistik yöntemler bakı-mından iyi tasarlanmaları gerekmektedir. Bir bilimsel çalışmanın her aşa-ması dikkatli ve özenli bir şekilde belirlenmelidir. Anahtar Kelimeler: McNemar testi; testin gücü; istatistiksel veri analizi; bağımlı iki örneklem ABSTRACT Objective: Cross-over design or paired case control studies that are using in clinical studies are the methods of design of experiments which requires dependent samples. The problem of sample size determination is generally difficult step of planning the statistical design. The aim of this study is to provide the researchers a practical approach for determining the sample size in paired control studies. Material and Methods: In this study, determination of sample size is mentioned in detail identification for the most common method Mc Nemar Test in paired (dependent) samples. Sample sizes are calculated according to different significance and power level combination. Results: Sample sizes were calculated for McNemar test which is used paired samples studies, for the combination of different power levels (1-β) and significance levels (α). The results are presented in tables. Conclusion: Appropriate sample sizes for the cross-over design or paired-control studies used in clinical studies were calculated and presented in tables. These tables are to provide the researchers a practical approach for determining the sample size in paired control studies.

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“…This further adds to the complexity of the problem regarding correct identification and differentiation of various respiratory-immunosuppressive agents complicating LPAI-ND disease complex. Several diagnostic methods such as isolation and serology are applied for detecting and differentiating various respiratory-immunosuppressive infections in poultry ( Luciano et al , 2011 ; Pawar et al , 2012 ; Bodhidatta et al , 2013 ; Saadat et al , 2014 ). However, the conventional diagnostic methods are time consuming and labor intensive.…”

Section: Introductionmentioning

confidence: 99%

Molecular Survey of Respiratory and Immunosuppressive Pathogens Associated with Low Pathogenic Avian Influenza H9N2 Subtype and Virulent Newcastle Disease Viruses in Commercial Chicken Flocks

et al. 2017

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The study was carried out in 48 poultry flocks to elucidate the roles of various complicating pathogens involved along with Newcastle disease (ND)/ low pathogenic avian influenza (LPAI) outbreaks. Necropsy was conducted and samples were collected for the isolation of Newcastle disease virus (NDV), Influenza A virus, infectious bronchitis virus (IBV), pathogenic bacteria; molecular detection of infectious laryngotracheitis virus (ILTV), fowl adeno virus (FAV), chicken anaemia virus (CAV), Mycoplasma synoviae (MS) and Mycoplasma gallisepticum (MG). The isolation results confirmed that 18/48 flocks (37%) were positive for the presence of hemagglutinating agents. Out of 18 hemagglutination (HA) positive flocks, 11 flocks (61%) were positive for both avian influenza virus (AIV) and NDV; 4 flocks (22%) were positive for NDV; and 3 flocks (17%) were positive for AIV. Sequence analysis of hemagglutinin and neuraminidase genes of AIV revealed that all were belonging to LPAI-H9N2 subtype. Sequence analysis of F gene of NDV revealed that they belong to virulent type. The PCR results confirmed the presence of three to seven etiological agents (CAV, FAV, ILTV, MG, MS and avian pathogenic E. coli along with LPAI/NDV from all the 18 HA-positive flocks. The detection rate of triple, quadruple, quintuple, sextuple and sevenfold infections was 17% (3 flocks), 28% (5 flocks), 11%, (2 flocks) 28% (5 flocks) and 17% (3 flocks), respectively. In conclusion, the disease complex involved more than one pathogen, primarily resulting from the interplay between LPAI-H9N2 and NDV; subsequently this could be exacerbated by co-infection with other agents which may cause exacerbated outbreaks that may otherwise go undetected in field.

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Comparative Study of Serological Tests forMycoplasma synoviaeDiagnosis in Commercial Poultry Breeders

Cited by 17 publications

References 18 publications

Mycoplasmosis in Poultry, a Perpetual Problem

Mycoplasmosis in Poultry, a Perpetual Problem

Practical Approaches For Determination Of Sample Size In Paired Case-Control Studies

Molecular Survey of Respiratory and Immunosuppressive Pathogens Associated with Low Pathogenic Avian Influenza H9N2 Subtype and Virulent Newcastle Disease Viruses in Commercial Chicken Flocks

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