Comparative Study of Spermatogenesis and Nucleolar Behavior in Testicular Lobes of<i>Euschistus heros</i>(Heteroptera: Pentatomidae)

Souza, Hederson Vinícius de; Itoyama, Mary Massumi

doi:10.1155/2010/428673

Cited by 7 publications

(13 citation statements)

References 39 publications

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“…In all the examined individuals, the fifth lobe was thinner than the others, and had spermatic cells larger in diameter than those of the first to third lobes but smaller than those of the fourth and sixth lobes, as reported by Souza and Itoyama (). The presence of spermatozoa with different sizes and diameters was corroborated in E. heros : Cossolin () described three types of sperms different in the morphology and ultrastructure.…”

Section: Discussionsupporting

confidence: 82%

“…The present study revealed that the testicules were essentially identical in morphology among individuals from the three experimental sources of E. heros , being approximately 2 mm in length and having six lobes as observed in specimens collected in the State of São Paulo by Souza and Itoyama (). The number of lobes varies from three to eight, with the prevalence of seven, among species of the family Pentatomidae (Souza & Itoyama ).…”

Section: Discussionsupporting

confidence: 76%

“…Previous cytogenetic studies reported the karyotype of 2 n = 14 (12A + XY) and a differentiated behavior of the sex chromosomes, as heteropycnosis, for E. heros (Souza & Itoyama ; Bardella et al ). The same karyotype and the regular pattern of meiotic chromosomal behavior were observed among all the three experimental groups in the present study.…”

Section: Discussionmentioning

confidence: 97%

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Effects of thiamethoxam and lambda‐cyhalothrin on spermatogenesis of Euschistus heros (Heteroptera: Pentatomidae)

Aguiar

Husch

Gallo

et al. 2017

Entomological Science

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Euschistus heros (Fabricius, 1798) is one of the most harmful insect pests damaging Brazilian soybean crops and has become a major problem due to its high population density and resistance to insecticides. Currently, there are no data on whether alterations of testicular morphology and chromosomal behavior are associated with the resistance mechanisms related to the action of insecticides. This study integrated analyses of the testicular morphology, meiocyte cell division, and chromosomal structure and behavior in the process of spermatogenesis in E. heros. We compared these features among wild‐caught individuals, insecticide‐susceptible and ‐resistant strains. The resistant strain was established through a selection experiment exposing the bugs to insecticides (thiamethoxam + lambda‐cyhalothrin) for 15 generations. No differences were detected in the examined features among the three groups of experimental individuals: the testis comprised six lobes, with the fifth lobe thinner than the others; the karyotype was 2n = 14 (12 + XY), with no evident changes in chromosomal breakage, rearrangement or behavior in the meiosis; and abundant spermatozoa were observed in all testicular lobes. Thus, any effects of the long‐term (15 generations) experimental selection by exposure to the insecticides were not detected on the male germinal tissue and chromosomes, suggesting the irrelevancy of the examined features to insecticide‐resistance mechanisms in E. heros.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 97%

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Effects of thiamethoxam and lambda‐cyhalothrin on spermatogenesis of Euschistus heros (Heteroptera: Pentatomidae)

Aguiar

Husch

Gallo

et al. 2017

Entomological Science

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“…According to the literature, the number of lobes in the family Lygaeidae is variable, with species with two, four, six and seven lobes, where seven is considered the ancestral number (Grozeva & Kuznetsova, 1992). According to the literature, Limnogonus aduncus (Gerridae, Castanhole et al, 2008) (Souza & Itoyama, 2010) includes six species of the subfamily Asopinae, in which only one species (Euthyrhynchus floridanus) has six testicular lobes and the others seven. In the other subfamilies, the distribution of lobes differs, ranging from four to eight in the Discocephalinae, four to five in the Edessinae and three to seven in the Pentatominae.…”

Section: Discussionmentioning

confidence: 99%

“…In the Discocephalinae, for example, the harlequin is usually lobe 5, numbered from the ejaculatory duct. In most cases, when lobes 4 and 6 show polymegaly, lobe 5 shows atypical meiosis, with the only exception being Euschistus heros (Pentatomidae: Carpocorini), which in addition to lacking a harlequin lobe, has a very narrow fifth lobe (Souza & Itoyama, 2010). The present study found that Proxys albopunctulatus (Pentatomidae: Carpocorini) belongs this select group, i.e., it lacks a harlequin lobe, and lobe 5 is flanked by lobes that produce differentiated cells and has a small diameter.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of the testes and spermatogenesis in species of the family Pentatomidae (Heteroptera)

Souza¹,

Murakami²,

Moura³

et al. 2011

Eur. J. Entomol.

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Abstract. There are few studies on the morphology and meiosis in the testicles of Heteroptera, but are extremely important, especially for the family Pentatomidae, because in some species in this family meiosis in the testicular lobes results in the production of non-fertile spermatozoa. With the aim of improving the level of understanding of this phenomenon the morphology of the testes and spermatogenesis in 10 species of Pentatomidae were recorded and compared. All of them have testes covered by a pigmented peritoneal sheath. In some species the internal membrane or just inside the peritoneal sheath is pigmented. The pigmentation of both membranes varied. The pigmentation of both was either yellow, or the internal membrane was yellowish and the external one reddish. When the membrane is pigmented, the colour is red or yellow. The number of lobes varied from 3 to 7, with intermediate numbers of 4 and 6. The size and diameter of the lobes are similar in all the species studied, except Proxys albopunculatus, in which the diameter of the third and fifth is smaller than that of the other lobes. The behaviour of the cells during spermatogenesis was the same in all lobes of most species, except in P. albopunctulatus, in which the harlequin lobe is absent and the cells in lobes 4 and 6 exhibited characteristics different from those of cells in lobes 1-3 and 5. Chlorocoris complanatus and Loxa deducta (both Pentatominae) have a harlequin lobe (lobe 5). The chromosome complements recorded were: 2n = 12 (10A + XY) in Dichelops melacanthus and Edessa collaris, 2n = 14 (12A + XY) in C. complanatus, Edessa meditabunda, Ladeaschistus sp., Loxa deducta, P. albopunctulatus, Piezodorus guildinii and Thyanta perditor and 2n = 16 (14A + XY) in Edessa affinis. Thus, this study extends the knowledge of characteristics, such as the pigmentation of the peritoneal sheath, number of testicular lobes, the occurrence of meiotic cells in some testicular lobes, and the chromosome complements of the family Pentatomidae.

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Morphology of the male reproductive system and sperm of Leptoglossus zonatus (Dallas, 1852) (Heteroptera: Coreidae)

Paulo,

Rezende,

Dias

et al. 2024

Microscopy Res & Technique

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Taxonomic data on Coreidae have been fragmented over time and need to be revised. Likewise, data related to the development of germ cells and the features of the male reproductive system, including sperm, will contribute to understanding the biological mechanisms of reproduction and the systematics of its representatives. Aiming to provide these data, we describe the morphology of the male reproductive system and spermatozoa of Leptoglossus zonatus using light and transmission electron microscopies, respectively. Each of the two testes is surrounded by a bright red‐pigmented sheath and formed by seven follicles arranged side by side. The two vasa deferentia are filled with individualized sperm, especially in their final portion, which is dilated and curved. After dilation, the vasa deferentia receive the ducts of the accessory glands of mesodermal origin. The other unpaired accessory gland is of ectodermal origin and opens into the ejaculatory duct. Both glandular types are densely coiled and have lumens filled with secreted material. Testicular follicles contain cysts with germ cells at different stages of spermatogenesis, indicating continuous production of gametes throughout adult life. Mature sperm measure around 310 μm long, with a nucleus of 36 μm and a flagellum formed only by an axoneme of 9 + 9 + 2 microtubules and two symmetrical mitochondrial derivatives. Like the sperm of other Heteroptera, the acrosome has a single structure (without perforatorium), there are no accessory bodies in the flagella, and the mitochondrial derivatives are connected to the axonemes, supporting the synapomorphic condition of these characteristics for this suborder of bedbugs.Research Highlights The Leptoglossus zonatus sperm are slender and long, about 310 μm in length, and a nucleus 36 μm long. Spermatogenesis occurs throughout adult life and equally in the seven testicular follicles. The centriole adjunct in L. zonatus sperm does not give rise to accessory bodies. The ectodermal gland produces a filamentous secretion, whereas in the ectodermal sac, the secretion is globular.

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Comparative Study of Spermatogenesis and Nucleolar Behavior in Testicular Lobes ofEuschistus heros(Heteroptera: Pentatomidae)

Cited by 7 publications

References 39 publications

Effects of thiamethoxam and lambda‐cyhalothrin on spermatogenesis of Euschistus heros (Heteroptera: Pentatomidae)

Effects of thiamethoxam and lambda‐cyhalothrin on spermatogenesis of Euschistus heros (Heteroptera: Pentatomidae)

Comparative analysis of the testes and spermatogenesis in species of the family Pentatomidae (Heteroptera)

Morphology of the male reproductive system and sperm of Leptoglossus zonatus (Dallas, 1852) (Heteroptera: Coreidae)

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