Comparative Study of Sterol Ester Synthesis using Thermomyces Lanuginosus Lipase in Stirred Tank and Packed‐Bed Bioreactors

Abstract

A comparative study was done on the production of different sterol esters using a stirred tank batch reactor (STBR) and packed bed reactor (PBR) using Thermomyces lanuginosus (Lipozyme TLIM) enzyme, a commercially immobilized lipase. Different oils were used as the sources of particular fatty acids, e.g., fish oil for n-3 polyunsaturated fatty acids (n-3 PUFA), linseed oil for alpha linolenic acid (ALnA) and mustard oil for erucic acid. Reaction parameters, such as substrate molar ratio, reaction temperature and enzyme concentration, were standardized in the STBR and maintained in the PBR. To provide equal time of residence between the substrate and enzyme in both the reactors for the same amount of substrates, the substrate flow rate in the PBR was maintained at 0.27 ml/min. Thin layer chromatography was used to monitor the reaction, and column chromatography was used to determine the product yields. Fatty acid compositions of the esters were determined by gas chromatography. The study showed that the packed bed bioreactor was more efficient than the batch reactor in sterol-ester synthesis with less migration of acyl groups.

Comparison of native and capric acid-enriched mustard oil effects on oxidative stress and antioxidant protection in rats

“…Capric acid-enriched mustard oil was prepared by the reaction between capric acid and mustard oil in a packed bed bioreactor using lipase TLIM as a catalyst ( 17 ) . Unreacted fatty acid was separated from the oil by vacuum distillation.…”

Optimization of Lipase-Catalyzed Transesterification of Cotton Seed Oil for Biodiesel Production Using Response Surface Methodology

“…Therefore, the use of an immobilized enzyme could be a good choice as it can provide enzyme reusability, hence reduces operational costs. It can also reduce enzyme contamination and facilitate easy separation of the products (Sengupta et al 2010). In addition, the stability, activity and reusability of enzymes can also be improved by immobilization.…”

Kinetics and Optimization of Lipophilic Kojic Acid Derivative Synthesis in Polar Aprotic Solvent Using Lipozyme RMIM and Its Rheological Study

“…The use of other types of bioreactors, such as packed bed, may also be useful for continuous process operation to improve the esterification performance [ 56 ]. Bioreactors with non-mechanical agitation systems may provide better enzyme protection than that usually observed in a stirred tank reactor by mechanical damage due to the high shear created by the use of the impeller [ 57 , 58 ]. The efficient and low-cost method for the separation and purification of KAD from the remaining substrates and other by products should be developed.…”