Comparative study of surface plasmon resonance, electrochemical and electroassisted chemiluminescence methods based immunosensor for the determination of antibodies against human growth hormone

Electrochemical biosensors for hormone analyses

“…For repeatability of the method they used eight antigen samples with the concentration of 100 pg/mL and RSD value was 1.77%. Ramanaviciene et al (2012) investigated the feasibility of their immunosensor for hGH detection in real samples. Serum was diluted 10 times with 10 mM PBS buffer, pH 7.4, and various concentrations of anti-hGH were added.…”

A label-free electrochemiluminescence immunosensor based on KNbO3–Au nanoparticles@Bi2S3 for the detection of prostate specific antigen

“…Moreover, the electrochemical methods could help to understand the emitting mechanism by the redox current. Thus, ECL has the advantages of chemical luminescence with the ability of being controlled easily, high sensitivity, good stability, high selectivity and intensity of the light-emitting reaction that is afforded by electrochemistry (Ramanaviciene et al, 2012;Richter, 2004). ECL has many advantages over spectroscopic-based techniques because it does not require the use of external light sources, so the problems derived by light scattering will be avoided (Bertoncello and Forster, 2009).…”

“…Some reports has researched the ECL properties of CdTe (Bae et al, 2004), CdSe (Myung et al, 2002), silicon nanocrystal quantum dots , CdSe/ZnSe (Myung et al, 2003), tris (2,2′-bipyridine) ruthenium (2 þ ) and oxalate or organic acids (Rubinstein and Bard, 1981) and so on. In addition, several authors have reported extremely sensitive ECL assays for bacterial and viral species (Bruno and Kiel, 2002), as well as cancer antigens (Ramanaviciene et al, 2012), environmental and food monitoring (Richter, 2004).…”

One-Step Quantitative Detection of Human Chorionic Gonadotropin by Integrating Immunochromatography Test Strip with Fluorescence Detection of Quantum Qots

“…Although methodological advances have been achieved, detection methods are still severely restricted [1] [2]. For example, conventional methods, such as enzyme-linked immunosorbent assays (ELISA) and chemiluminesc ence immunoassay (CLIA), suffer from the disadvantages of being time-consuming, having low sensitivity and a laborious process.…”