Comparative study of the effects of heavy metals on embryonic development of zebrafish

Gouva, Evangelia; Nathanailides, Cosmas; Skoufos, Ioannis; Paschos, Ioannis; Athanassopoulou, F.; Pappas, Ioannis S.

doi:10.1111/are.14660

Cited by 18 publications

(8 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heavy metals directly affect the systems of the plasma membrane that maintain the membrane potential, and their sensitivity to ions changes over time. From the heavy metals used in the various concentrations, zinc was proved to be the most toxic heavy metal causing increased risk of mortality and shorter life expectancy for zebrafish eggs (Gouva et al, 2020). In the diagrams, this assumption is represented by small values the correlations at large time shifts.…”

Section: Discussionmentioning

confidence: 99%

“…Such forms of chemicals, even at submolar concentrations, cause metabolic disorders, carcinogenic and toxic effects as well as structural changes, the development of abnormal behaviour or death (Singh et al, 2011;Seneviratne et al, 2019;Gökalp et al, 2020;Yoo et al, 2020). A wide range of negative changes in the viability of the fish embryo under influence of the heavy metals has been found (Martinez et al, 2017;Klein et al, 2019;Gouva et al, 2020). One of the effects of the heavy metals on early embryo development is the occurrence of embryonic deformities following fertilization (Kerekes et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mathematical model of transmembrane potential dynamics of loach early embryogenesis

Galyk

Fedorovych

Lychkovsky

et al. 2021

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Heavy metals in the water environment are known to have a negative effect on the viability of fish in early development. We have discussed the influence of environmental factors on early embryo development from the viewpoint of the correlation adaptometry method. The analysis of time series with the subsequent construction of a mathematical model was used to determine the change in the greatest effect of certain types of ions on the values of the transmembrane potential for prognostic purposes. The membrane potential is accepted as an integral indicator of the state of the embryos. Structures of five elements of the same type were constructed for the time shifts from 0 to 180 minutes. Each element in the system characterizes the value of the transmembrane potential that was measured in a cell incubated in one of the five solutions during early embryo development. Mathematical models describing the cell membrane potential dynamics have been created and studied. It was noted that the transmembrane potential dynamics of embryo cells is dependent on a change in the value of the correlation coefficient between elements of the system. A decrease in the sum of the correlations between individual elements of the system with an increase in the magnitude of the time shift is established. The results of the numerical solutions of the system equations indicated the sequence of changes in the greatest effect of the incubation medium on the value of the membrane potential in cells. The study of the membrane potentials’ dynamics, using the total values of the strength of correlation, confirmed the influence of heavy metals in the incubation medium on the membrane potential of embryo cell in early development.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mathematical model of transmembrane potential dynamics of loach early embryogenesis

Galyk

Fedorovych

Lychkovsky

et al. 2021

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

show abstract

“…It is important to assess the safety risks of different concentrations and combinations of heavy metals and to elucidate their potential toxicity mechanisms. Studies have shown the effects of heavy metals on zebrafish embryo hatchability, life expectancy, heart rate, and bone development (Gouva et al., 2020). The applications of zebrafish in heavy metal evaluation are summarized in Figure 4 and Table 4.…”

Section: Research Progress In the Toxicological Assessment Of Hazards...mentioning

confidence: 99%

Zebrafish as model organisms for toxicological evaluations in the field of food science

Hou

Liu

Qin

et al. 2023

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Food safety has long been an area of concern. The selection of stable and efficient model organisms is particularly important for food toxicology studies. Zebrafish (Danio rerio) are small model vertebrates, and 70% of human genes have at least one zebrafish ortholog. Zebrafish have advantages as model organisms due to their short life cycle, strong reproductive ability, easy rearing, and low cost. Zebrafish embryos have the advantage of being sensitive to the breeding environment and thus have been used as biosensors. Zebrafish and their embryos have been widely used for food toxicology assessments. This review provides a systematic and comprehensive summary of food toxicology studies using zebrafish as model organisms. First, we briefly introduce the multidimensional mechanisms and structure–activity relationship studies of food toxicological assessment. Second, we categorize these studies according to eight types of hazards in foods, including mycotoxins, pesticides, antibiotics, heavy metals, endocrine disruptors, food additives, nanoparticles, and other food‐related ingredients. Finally, we list the applications of zebrafish in food toxicology studies in line with future research prospects, aiming to provide a valuable reference for researchers in the field of food science.

show abstract

“…Fish can be negatively affected by the heavy metal in the first production stage, and eventually, metals can be transferred to the human body by eating fish. Some of the adverse impacts are impaired survival rate, growth and reproduction (Gouva et al, 2020; Ren et al, 2019) and, eventually, economic loss. Although humans should focus on the decline of loading metals in water, another mitigation strategy can be an improvement of the fish health in any stressed environmental conditions by metals.…”

Section: Introductionmentioning

confidence: 99%

“…of the adverse impacts are impaired survival rate, growth and reproduction (Gouva et al, 2020;Ren et al, 2019) and, eventually, economic loss. Although humans should focus on the decline of loading metals in water, another mitigation strategy can be an improvement of the fish health in any stressed environmental conditions by metals.…”

mentioning

confidence: 99%

EDTA detoxifies heavy metals on exposed beluga ( Huso huso ) with pollution stress: Growth performance, immunohaematology, blood biochemistry and antioxidant activity

Falahatgar

Javadian

Soleymani

et al. 2021

Aquaculture Research

View full text Add to dashboard Cite

Ethylenediaminetetraacetic acid (EDTA) is one of the most popular chelating agents. This study was conducted to investigate the effect of EDTA on the growth and health physiology of beluga (38.47 ± 0.58 g) before and after heavy metal stress (HMS). Five experimental diets, including control, 0.5EDTA, 1EDTA, 1.5EDTA and 2EDTA, were formulated to feed fish for eight weeks. The results showed that fish was fed with dietary 1.5EDTA (108.37 g) had a higher weight gain as compared with control (p < 0.05). Blood performance in the control group after HMS decreased but not in fish fed supplemented diets with EDTA. EDTA reduced uric acid and creatinine levels in beluga. Fish fed dietary 1.5EDTA had a higher value of blood performance (55.17), glucose (50.3 mg/dl), superoxide dismutase (SOD U/ml) (75.8) and glutathione peroxidase (234.0 U/ml) after HMS as compared with the control. Additionally, lower values of uric acid (3.05 mg/dl), creatinine (0.27 mg/dl) and AST (61.47 U/ml) after HMS in the 1.5EDTA group were observed (p < 0.05). Correlation analysis revealed a strong positive correlation between EDTA level and protein efficiency, lipid efficiency, and blood performance and a strong negative correlation with some parameters after stress, including SOD, glucose, uric acid, creatinine and aspartate transaminase (p < 0.01). In conclusion, feeding fish with the 1.5EDTA diet improved growth and detoxified heavy metals.

show abstract

Comparative study of the effects of heavy metals on embryonic development of zebrafish

Cited by 18 publications

References 58 publications

Mathematical model of transmembrane potential dynamics of loach early embryogenesis

Mathematical model of transmembrane potential dynamics of loach early embryogenesis

Zebrafish as model organisms for toxicological evaluations in the field of food science

EDTA detoxifies heavy metals on exposed beluga ( Huso huso ) with pollution stress: Growth performance, immunohaematology, blood biochemistry and antioxidant activity

Contact Info

Product

Resources

About