Comparative study of the physiological roles of three peroxidases (NADH peroxidase, Alkyl hydroperoxide reductase and Thiol peroxidase) in oxidative stress response, survival inside macrophages and virulence of <i>Enterococcus faecalis</i>

Carbona, Stéphanie La; Sauvageot, Nicolas; Giard, Jean‐Christophe; Benachour, Abdellah; Posteraro, Brunella; Auffray, Yanick; Sanguinetti, Maurizio; Hartke, Axel

doi:10.1111/j.1365-2958.2007.05987.x

Cited by 133 publications

(132 citation statements)

References 55 publications

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“…When required, erythromycin (150 mg ml 21 ) was added to E. faecalis cultures. Cultures of E. faecalis strains were also performed on CCM17 MOPS medium with glycerol (0.5 %, v/v) as the sole carbohydrate (CCM17-gly), as described by La Carbona et al (2007). Different pH conditions (4.0-9.0) were tested using microplates with CCM17-gly medium.…”

Section: Methodsmentioning

confidence: 99%

The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence

et al. 2012

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Enterococcus faecalis is an opportunistic pathogen responsible for nosocomial infections. Lipoproteins in Gram-positive bacteria are translocated across the plasma membrane and anchored by the fatty acid group. They perform critical roles, with some described as virulence determinants. The aim of this study was to explore the roles of E. faecalis lipoproteins in the stress response and virulence. We constructed a mutant affected in the predicted prolipoprotein diacylglyceryl transferase gene lgt, and examined the role of Lgt in membrane anchoring, growth, the stress response and virulence. Inactivation of lgt enhanced growth in a high concentration of Mn2+ or under oxidative stress in vitro, and significantly decreased virulence

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Section: Methodsmentioning

confidence: 99%

The prolipoprotein diacylglyceryl transferase (Lgt) of Enterococcus faecalis contributes to virulence

et al. 2012

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“…The most conserved oxidative resistance mechanism in LAB results from the coupling of NADH oxidase and NADH peroxidase (184 (247). Multiple enzymes with peroxidase activity have been described for LAB, such as AhpCF, Npr, and Tpx of E. faecalis and AhpCF and Tpx of S. mutans (248).…”

Section: Detoxification Of Ros In Labmentioning

confidence: 99%

“…An alternative strategy to counteract superoxide anion radicals is by the production of high levels of intracellular glutathione (252). Various enzymes that are known to counter oxidative stress, namely, MsrAB, AhpCF, Tpx, Npr, KatA, Sod, and HypR, have been characterized for E. faecalis (94,248,(253)(254)(255).…”

Section: Detoxification Of Ros In Labmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

541

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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“…Other reports indicate that Tpx is expressed extracellularly by Mycobacterium tuberculosis (84), Actinobacillus actinomycetemcomitans (38), and Bacillus anthracis (2) and periplasmically in E. coli (16), despite the apparent absence of a signal sequence. Interestingly, Tpx also appears to play a role in protecting Enterococcus faecalis against phagocytosis (62). If analogous to H. pylori, this may explain, in part, H. pylori's resistance to phagocytic digestion.…”

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confidence: 99%