Comparative study of the properties of lutein nanoliposomes coated with chitosan/(−)‐epigallocatechin‐ 3‐gallate (EGCG) complexes

Abstract

BACKGROUND Numerous positive effects have been attributed to lutein, a lipophilic nutrient, including resisting ultraviolet radiation and protecting retinal pigment epithelial (RPE) cells against blue light damage. It also has preventive effects against cardiovascular disease and cancer. However, its use could be limited by its poor stability and low bioaccessibility in the human digestive system. An encapsulation delivery system was therefore developed to resolve these limitations. In this study, chitosan‐modified lutein nanoliposomes (CS‐LNLs), chitosan‐EGCG covalently modified lutein nanoliposomes (C‐CS‐EGCG‐LNLs), and chitosan‐EGCG noncovalently modified lutein nanoliposomes (non‐C‐CS‐EGCG‐LNLs) were designed. The average particle size, ζ‐potential, and retention of lutein during storage were measured to indicate the physicochemical stability of the modified lutein nanoliposomes. The bioaccessibility of modified lutein nanoliposomes was also investigated to demonstrate the availability of lutein in the human digestive system. RESULTS First, Fourier‐transform infrared spectroscopy (FTIR) verified that covalent bonds between chitosan and EGCG were formed. Subsequently, ζ‐potential results revealed that C‐CS‐EGCG‐LNLs had a relatively stable structure in comparison with lutein nanoliposomes (LNLs). The retention rate of lutein in CS‐LNLs, C‐CS‐EGCG‐LNLs, and non‐C‐CS‐EGCG‐LNLs was improved, especially in C‐CS‐EGCG‐LNLs (at around 70% of lutein in initial system). An in vitro digestion experiment illustrated that CS‐LNLs, C‐CS‐EGCG‐LNLs, and non‐C‐CS‐EGCG‐LNLs presented relatively higher bioaccessibility, especially in C‐CS‐EGCG‐LNLs (at around 33% of luein in initial system), which increased 2.5 and 1.65 times in comparison with free lutein and LNLs, respectively. CONCLUSION Overall, the results showed that C‐CS‐EGCG‐LNLs presented greater physicochemical stability and bioaccessibility than LNLs, CS‐LNLs, and non‐C‐CS‐EGCG‐LNLs. © 2023 Society of Chemical Industry.