Comparative study of the xenobiotic metabolising system in the digestive gland of the bivalve molluscs in different aquatic ecosystems and in aquaria experiments

Petushok, N. E.; Gabryelak, Teresa; Palecz, Danuta; Zavodnik, L.B.; Varga, I; Deér, K. Aranka

doi:10.1016/s0166-445x(02)00030-9

Cited by 36 publications

(17 citation statements)

References 21 publications

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“…In the south coast of Ireland, Fitzpatrick et al (1997) did not observe any significant seasonal changes in GST activity in mussels. No differences between sampling sites or any clear temporal seasonal trends for GST activity in mussels were observed from the western Mediterranean Sea by Porte et al (2001), neither were they found in a lake and rivers in the Tucholski Landscape Park (central Poland) by Petushok et al (2002). Seasonal changes in CAT activity in mussels have been reported by Solé et al (1995), Regoli (1998), Narbonne et al (1999) and Leiniö and Lehtonen (2005), all showing a higher CAT activity in spring compared to autumn, similar to this study.…”

Section: Factors Influencing Biomarker Levels In Flounder In the Gulfsupporting

confidence: 88%

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)

Kopecka¹,

Lehtonen²,

Baršienė³

et al. 2006

Marine Pollution Bulletin

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In the framework of the EU funded BEEP project a set of biomarkers, gross morphometric indices and tissue concentrations of selected organic pollutants were measured in flounder (Platichthys flesus) and mussels (Mytilus trossulus) collected twice a year (April and October) from three sites in the inner Gulf of Gdań sk between autumn 2001 and spring 2003. In flounder, seasonal differences in most biomarkers were observed, but no correlations with tissue pollutant levels could be found. In mussels, highly variable levels in biomarker responses were seen, but no clear seasonal or spatial trends, directly related to tissue concentrations, could be established. The observed biomarkers distribution the study sites are probably mostly caused by interannual, seasonal and individual variability and, in case of flounder, possibly by exchange of stocks between the sampling sites.

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Section: Factors Influencing Biomarker Levels In Flounder In the Gulfsupporting

confidence: 88%

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)

Kopecka¹,

Lehtonen²,

Baršienė³

et al. 2006

Marine Pollution Bulletin

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“…Wilbrink et al (1991b) did not observe induction of P450 activity by either PB or 3MC in the digestive glands of pond snails. Both P450 and NADPH ferricyanide reductase activity was induced in microsomal fractions from the digestive glands of freshwater mussels by exposure to 2,4-dichlorophenol (Petushok et al 2002). Benzo[a]pyrene (7), Aroclor 1254 (PCB congeners), and crude oil, including PAHs, acted to significantly induce P450 activity in the polychaete Nereis virens (Lee 1981).…”

Section: Oxidasesmentioning

confidence: 99%

“…To clarify the relative distribution of GST between microsomal and cytosolic fractions, thorough washing of the microsomal fraction is necessary; such washing excludes contamination resulting from the residual cytosolic GST (Wilbrink et al 1991a). In bivalve, GST activity is predominantly located in digestive glands and visceral mass (Fitzpatrick and Sheehan 1993;Petushok et al 2002;Vidal and Narbonne 2000;Wilbrink et al 1991a). In gumboot chiton, more activity was observed in intestine than in digestive glands (Schlenk and Buhler 1988).…”

Section: Glutathione-s-transferases (Gst)mentioning

confidence: 99%

“…CDNB and pentachlorophenol (32) enhanced GST activity toward CDNB in daphnids, but CDNB did not modulate activity toward EHTA (LeBlanc and Cochrane 1985). 2,4-Dichlorophenol also increased its activity toward CDNB in the digestive glands of freshwater mussels (Petushok et al 2002) and in marine diatoms (Yang et al 2002). PB enhanced the GST activity in D. magna by a factor of two, while the conjugation rate with glutathione for CDNB increased by only 30% (Baldwin and LeBlanc 1996).…”

Section: Glutathione-s-transferases (Gst)mentioning

confidence: 99%

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Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Katagi

2009

Reviews of Environmental Contamination and Toxicology

137

102

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The ecotoxicological assessment of pesticide effects in the aquatic environment should normally be based on a deep knowledge of not only the concentration of pesticides and metabolites found but also on the influence of key abiotic and biotic processes that effect rates of dissipation. Although the bioconcentration and bioaccumulation potentials of pesticides in aquatic organisms are conveniently estimated from their hydrophobicity (represented by log K(ow), it is still indispensable to factor in the effects of key abiotic and biotic processes on such pesticides to gain a more precise understanding of how they may have in the natural environment. Relying only on pesticide hydrophobicity may produce an erroneous environmental impact assessment. Several factors affect rates of pesticide dissipation and accumulation in the aquatic environment. Such factors include the amount and type of sediment present in the water and type of diet available to water-dwelling organisms. The particular physiological behavior profiles of aquatic organisms in water, such as capacity for uptake, metabolism, and elimination, are also compelling factors, as is the chemistry of the water. When evaluating pesticide uptake and bioconcentration processes, it is important to know the amount and nature of bottom sediments present and the propensity that the stuffed aquatic organisms have to absorb and process xenobiotics. Extremely hydrophobic pesticides such as the organochlorines and pyrethroids are susceptible to adsorb strongly to dissolved organic matter associated with bottom sediment. Such absorption reduces the bioavailable fraction of pesticide dissolved in the water column and reduces the probable ecotoxicological impact on aquatic organisms living the water. In contrast, sediment dweller may suffer from higher levels of direct exposure to a pesticide, unless it is rapidly degraded in sediment. Metabolism is important to bioconcentration and bioaccumulation processes, as is detoxification and bioactivation. Hydrophobic pesticides that are expected to be highly stored in tissues would not be bioconcentrated if susceptible to biotic transformation by aquatic organisms to more rapidly metabolized to hydrophilic entities are generally less toxic. By analogy, pesticides that are metabolized to similar entities by aquatic species surely are les ecotoxicologically significant. One feature of fish and other aquatic species that makes them more relevant as targets of environmental studies and of regulation is that they may not only become contaminated by pesticides or other chemicals, but that they constitute and important part of the human diet. In this chapter, we provide an overview of the enzymes that are capable of metabolizing or otherwise assisting in the removal of xenobiotics from aquatic species. Many studies have been performed on the enzymes that are responsible for metabolizing xenobiotics. In addition to the use of conventional biochemical methods, such studies on enzymes are increasingly being conducted using immunochemical met...

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“…These increases become obvious with exposure to xenobiotic agents (Petushok et al 2002), and it should be remembered that these enzymes metabolize not only xenobiotics (hydrocarbons, pesticides, drugs) but a wide variety of substrates including endogenous molecules (fatty acids, eicosenoids, steroids) (Snyder 2000). This multi-enzymatic system contains different forms of cytochrome P450, cytochrome P450 reductase, and cytochrome b 5 and cytochrome b 5 reductase.…”

Section: Fatty Acidsmentioning

confidence: 99%

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

Labarta

Fernández‐Reiriz²,

Garrido³

et al. 2005

Mar. Ecol. Prog. Ser.

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Postlarval recruits of mussels Mytilus galloprovincialis were sampled in February 2003 at 7 localities along the Galicia coast (NW Spain) exhibiting different levels of impact from the 'Prestige' oil spill. These localities are important grounds for seed collection used in raft culture. The concentrations of polycyclic aromatic hydrocarbons (PAHs) as well as different biochemical and ecophysiological variables were determined in mussel tissues, as indicators of the toxic impact of the spilled oil. The following parameters were measured: size frequency distributions and size-weight relationships, survival in air, biochemical composition (proteins, carbohydrates, glycogen and lipids), lipid classes (phospholipids, sterol esters and waxes, triacylglycerols, free fatty acids and sterols), and fatty acid composition. The study determined sub-lethal effects in wild mussel seed populations sampled 3 mo after the oil spill. These effects are shown in the form of survival indices and alterations of lipid metabolism, especially in trygliceride and sterol fractions, as well as in saturated/monounsaturated fatty acid ratios. Monitoring these effects in wild mussel recruits is important for analysing growth, production and biochemical reserve cycle throughout the raft cultivation period and evaluating the capacity of the individuals to repair alterations detected in the juvenile stage. KEY WORDS: Mussel · Postlarvae · 'Prestige' · Toxic impact · PAH · Biochemical analysisResale or republication not permitted without written consent of the publisher

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Comparative study of the xenobiotic metabolising system in the digestive gland of the bivalve molluscs in different aquatic ecosystems and in aquaria experiments

Cited by 36 publications

References 21 publications

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

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Comparative study of the xenobiotic metabolising system in the digestive gland of the bivalve molluscs in different aquatic ecosystems and in aquaria experiments

Cited by 36 publications

References 21 publications

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach

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Response of mussel recruits to pollution from the Prestige oil spill along the Galicia coast. A biochemical approach