Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction

Rahnama, Hassan; Sattarzadeh, A.; Kazemi, Foad; Ahmadi, Najmeh; Sanjarian, Forough; Zand, Zahra

doi:10.1016/j.ab.2016.08.029

Cited by 24 publications

(8 citation statements)

References 26 publications

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“…Finally, the overall efficiency of different MNPs (Fe 3 O 4 , Fe 3 O 4 /SiO 2 , Fe 3 O 4 /SiO 2 /TiO 2 ) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO 4 , FeSO 4 /SiO 2 , FeSO 4 /SiO 2 /TiO 2 ) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].…”

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confidence: 72%

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Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Rahnama¹,

Sattarzadeh²,

Kazemi³

et al. 2016

Data in Brief

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In this dataset we integrated figures related to bacterial transformation using pBI121 plasmid and complementary analysis for magnetic nanoparticles (MNPs) characterizations. The structural map of pBI121 plasmid was drawn by Vector NTI software using the complete sequence of binary vector pBI121. Escherichia coli bacteria transformed using pBI121 plasmid and were grown on the selection media containing kanamycin.MNPs were characterized by energy dispersive spectroscopy (EDS) and transmission electron microscopy (TEM). Finally, the overall efficiency of different MNPs (Fe3O4, Fe3O4/SiO2, Fe3O4/SiO2/TiO2) in plasmid DNA isolation was compared using gel electrophoresis analysis. The data supplied in this article supports the accompanying publication “Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction” (H. Rahnama, A. Sattarzadeh, F. Kazemi, N. Ahmadi, F. Sanjarian, Z. Zand, 2016) [1].

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confidence: 72%

“…Five nanograms (5 ng) of pBI121 (Fig. 1) was gently mixed with competent E. coli cells [1]. After incubation on ice for 30 min, the bacteria were incubated at 42 °C for 90 s and then kept on ice for 2 min.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Rahnama¹,

Sattarzadeh²,

Kazemi³

et al. 2016

Data in Brief

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“…Nowadays, the use of various solid‐phase materials for DNA extraction has become attractive. In previous studies, polymeric ionic liquid microspheres , magnetic nanoparticles and gold nanoparticles were used as SPE agents for DNA separation. However, the agglomeration of nanoparticles is unavoidable in practical applications, which has a negative influence on the adsorption efficiency and capacity for DNA.…”

Section: Introductionmentioning

confidence: 99%

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Chen

Wang

Song

et al. 2018

Separation Science Plus

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A three‐dimensional reduced graphene oxide porous aerogel with packaged TiO2 nanoparticles was prepared by a simple one‐step self‐assembly in a water bath at a mild temperature. Herein, we overcame the aggregation of graphene layers by forming three‐dimensional architecture, and utilized TiO2 aggregates as blocks to make the composite surface uneven. The extended aromatic areas contribute to DNA adsorption by aromatic stacking and hydrophobic interaction. The obtained composite exhibited porous structure and was positively charged at a low pH. The composite exhibited a high capacity of 890.1 mg g−1 and an adsorption efficiency of 98.4% to DNA at pH 3, however, bovine serum albumin at the same concentration level was hardly adsorbed. The composite showed favorable selectivity to DNA against protein even designed in such a simple manner. The retained DNA could be stripped by using a 0.04 mol L−1 Britton–Robinson buffer at pH 10, giving rise to a recovery of 96.3%. The DNA was isolated from human whole blood to be used as templates for PCR amplification successfully. This illustrates that DNA with high purity and quality was obtained.

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“…We obtained pH 8 and 1.5 M NaCl as an optimal condition for desorption of DNA from MNPs [4]. The result further showed that, 0.2 mg nano-particle and 10 min at 55 °C are the optimal conditions for DNA desorption from nano-particles [4].…”

Section: Resultsmentioning

confidence: 73%

Novel kit for efficient extraction of long-segment genomic DNA by nanodisk adsorption

Han²,

Qiu³

2019

J. Appl. Sci. Eng. Technol. Educ.

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The acquisition of complete DNA is usually the first and most critical step in many basic molecular biology applications. Isolation of complete total DNA from tissue samples is related to different physical and biochemical properties of tissue. Long fragments of DNA are easily degraded and fragmented, which poses a challenge for extracting complete long fragments of DNA. By comparing several different extraction methods and verifying the concentration and length of extracted DNA, it is proved that the kit we used has high efficacy. We provide tissue kit DNA extraction for long fragments and obtain long fragments of high purity DNA from almost any type of tissue, especially muscle and blood tissues.

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Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction

Cited by 24 publications

References 26 publications

Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Novel kit for efficient extraction of long-segment genomic DNA by nanodisk adsorption

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Comparative study of three magnetic nano-particles (FeSO4, FeSO4/SiO2, FeSO4/SiO2/TiO2) in plasmid DNA extraction

Cited by 24 publications

References 26 publications

Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Dataset of plasmid DNA extraction using different magnetic nanoparticles (MNPs)

Reduced graphene oxide aerogel with packaged TiO2 nanoparticles as a promising adsorbent for the separation of DNA from human whole blood

Novel kit for efficient extraction of long-segment genomic DNA by nanodisk adsorption

Contact Info

Product

Resources

About

Reduced graphene oxide aerogel with packaged TiO₂ nanoparticles as a promising adsorbent for the separation of DNA from human whole blood