Comparative study on alkaloids and their anti-proliferative activities from three Zanthoxylum species

Abstract

BackgroundAlkaloids have been considered as the most promising bioactive ingredients in plant species from the genus Zanthoxylum. This study reports on the compositions and contents of the Zanthoxylum alkaloids (ZAs) from three Zanthoxylum species, and their potential anti-proliferation activities.MethodsAn HPLC-UV/ESI-MS/MS method was established and employed to analyze the alkaloids in different Zanthoxylum extracts. The common and unique peaks and their relative contents were summarized and compared to evaluate the similarity and dissimilarity of the three Zanthoxylum species. Meanwhile, inhibitory activity tests to four carcinoma cell lines, i.e., stomach tumor cells (SGC-7901), cervical tumor cells (Hela), colon tumor cells (HT-29) and Hepatic tumor cells (Hep G2), were carried out in vitro to evaluate the bioactivities of the ZAs.ResultsSeventy peaks were detected in the crude total alkaloid samples, and 58 of them were identified. As a result, 13 common peaks were found in the extracts of all the three Zanthoxylum species, while some unique peaks were also observed in specific species, with 17 peaks in Z. simulans, 15 peaks in Z. ailanthoides and 11 peaks in Z. chalybeum, respectively. The comparison of the composition and relative contents indicated that alkaloids of benzophenanthridine type commonly present in all the three Zanthoxylum species with high relative contents among the others, which are 60.52% in Z. ailanthoides, 30.52% in Z. simulans and 13.84% in Z. chalybeum, respectively. In terms of activity test, Most of the crude alkaloids extracts showed remarkable inhibitory activities against various tumor cells, and the inhibitory rates ranged from 60.71 to 93.63% at a concentration of 200 μg/mL. However, SGC-7901 cells seemed to be more sensitive to the ZAs than the other three cancer cells.ConclusionThe alkaloid profiles detected in this work revealed significant differences in both structures and contents among Zanthoxylum species. The inhibitory rates for different cancer cells in this study indicated that the potential anti-cancer activity should be attributed to quaternary alkaloids in these three species, which will provide great guidance for further exploring this traditional medicinal resource as new healthcare products.

Compostos químicos isolados de extratos e óleos essenciais do gênero Zanthoxylum Linnaeus (Rutaceae) e seu potencial antimicrobiano

“…Análises fitoquímicas das cascas do tronco de Z. chalybeum revelaram a presença de benzofenantridina 132 145, 8-O-demethylchelerythrine (146), 9,10-didemethylchelerythrine (147), 6-hydroxy-10-methylsanguinarine (148), 2-dimethoxy-3hydroxybeugenine (149), (-) isotembetina (150), 10-hydroxy-2,3,9,12-tetramethoxy-jatrorrhizine (151) e (+) -N-metilplatidamina (152) (figura 1) (Kato et al 1996, Tian et al 2017.…”

Influence of Chelidonium majus Water Extract onto Cancer Cells and Platelets

“…Thus, we obtained the extract of C. majus that was characterized and lyophilized for further studies. It contained mostly alkaloids precursors of berberine and chelerythrine that were identified using MALDI-TOF mass-spectrometry [36][37][38][39].…”

Antimicrobial activity of flavonoids glycosides and pyrrolizidine alkaloids from propolis of Scaptotrigona aff. postica

“…LubSolutions software (Shimadzu, Tokyo, Japan) was used for system control and data analysis. The data obtained were compared to MS data reported by El-Shazly and Wink (2014), Kopp et al, (2020),Mädge et al, (2020),Tian et al, (2017,Coelho et al,…”