Comparative study on Cronobacter sakazakii and Pseudomonas otitidis isolated from septic tank wastewater in microbial fuel cell for bioelectricity generation

Thulasinathan, Boobalan; Samsudeen, N.; Samuel, James Obeth Ebenezer; Soorangkattan, Saravanan; Muthuramalingam, Jothi Basu; Mohanrasu, K.; Ravindran, Balasubramani; Nguyen, Dinh Duc; Chang, Soon Woong; Bolan, Nanthi; Tsang, Yiu Fai; Amábilis-Sosa, Leonel Ernesto; Arun, A.

doi:10.1016/j.fuel.2019.03.060

Cited by 48 publications

(27 citation statements)

References 65 publications

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“…The CE is comparable with recently published studies. [22][23][24] This data shows that sewage sludge was a suitable substrate for electroactive microorganisms. In addition to the anode potential, power density, and polarization curves were used to evaluate the performance of the SMFC (Figure 2b).…”

Section: Scanning Electron Microscope and Fluorescence In Situ Hybrmentioning

confidence: 72%

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO₂ electrode

Taşkan

Hasar

2020

Env Prog and Sustain Energy

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The aim of this study is to increase the electricity generation capacity of sediment microbial fuel cell (SMFC) using sewage sludge as a substrate source in anode media via an attractive electrode material. For this purpose, several electrochemical and molecular techniques have been employed to evaluate the performance of SMFC. The max power density and charge transfer resistance of SMFC running on sewage sludge were 187 mW/m2 and 84.7 Ω, respectively. The polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE) analysis indicated that bacteria in anode biofilm belonged to the Clostridia, d‐proteobacteria, g‐proteobacteria, and b‐proteobacteria. Scanning electron microscopy and fluorescence in situ hybridization observations demonstrated that a viable biofilm was formed on the anode surface and the abundance of bacteria was significantly higher than archaea. Consequently, this study showed that sewage sludge could be used as a substrate in the anode media of SMFC for producing high power density.

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Section: Scanning Electron Microscope and Fluorescence In Situ Hybrmentioning

confidence: 72%

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO₂ electrode

Taşkan

Hasar

2020

Env Prog and Sustain Energy

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“…It can be seen that the bacteria are well stabilized on graphite granules due to the interaction of electrostatic and van der Waals forces, as well as extracellular polymeric substance (as seen in Fig. 5c and d) and the development of bacterial cells 69 . Furthermore, most bacteria appear to have the same morphology and are most likely to be rod‐shaped due to the acclimation and immobilization of specific species in the anodic chamber having analogous characteristics.…”

Section: Resultsmentioning

confidence: 88%

“…5c and d) and the development of bacterial cells. 69 Furthermore, most bacteria appear to have the same morphology and are most likely to be rod-shaped due to the acclimation and immobilization of specific species in the anodic chamber having analogous characteristics. Thus, we conclude that the graphite granule is a suitable matrix for the development of biofilm, thereby assisting electricity generation in UTMFC.…”

Section: A Critical Nitrate Concentration Was Presented By Kashima Andmentioning

confidence: 99%

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

Davarpanah

Sarbisheh

Sharghi

2020

J of Chemical Tech & Biotech

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BACKGROUND: A high nitrate concentration in groundwater in some parts of Iran has been detected due to untreated wastewater discharge and agriculture activities. Therefore, the development of treatment technology in this area using a new biological method was carried out. RESULTS: Activated sludge originating from slaughterhouse wastewater treatment plants and immobilized on graphite granules exhibited noticeable denitrification activity in a double chamber upflow tubular microbial fuel cell (UTMFC). The effects of three C/N values at different initial nitrate concentrations and external resistances on nitrate and chemical oxygen demand (COD) removal efficiencies as well as cell performance were studied at a fixed hydraulic retention time (HRT) of 12 h. Based on nucleotide sequence of 16S rRNA, four bacterial strains from anodic biofilm were also identified. The UTMFC operated in a continuous mode with C/N values of 20 and 10 was able to denitrify up to 150 mg L-1 nitrate without more accumulation of nitrite and ammonium ions than is permitted for drinking water. The maximum nitrate removal capacity of 0.25 ± 0.01 kg NO 3 −-N/ (m 3 net anodic compartment 'NAC'.day) was achieved at 400 mg L-1 initial nitrate concentration and at C/N = 10. Additionally, the maximum current density and power generation at this ratio were found to be 46.04 A m-3 NAC and 16.96 W/m 3 NAC, respectively. No efficient denitrification was observed at C/N = 5 while nitrite/ammonium formation increased. CONCLUSION: The experimental data documented the feasibility of bioelectricity generation and substrate removal utilizing acetate containing water under the adopted operating conditions of UTMFC, and the proposed system allowed the simultaneous heterotrophic denitrification/electricity generation.

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“…Pseudomonas protegens CHA0 was the nearest neighbor with a high similarity (99.79%) of 16S rRNA sequence. Some exoelectrogenic strains in the Pseudomonas genus have been isolated and characterized, such as P. aeruginosa PAO1 [24], P. otitidis AATB4 [25], P. monteilii LZU-3 [26], P. sp. C27 [27], P. alcaliphila MBR [28], P. putida ATCC 49128, and P. fluorescens MTCC 2269 [29,30].…”

Section: Identification Of the Isolated Strainmentioning

confidence: 99%

Recovery of Metals from Acid Mine Drainage by Bioelectrochemical System Inoculated with a Novel Exoelectrogen, Pseudomonas sp. E8

Hou

Zhang

et al. 2019

Microorganisms

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Acid mine drainage (AMD) is a typical source of environmental pollution ascribing to its characteristics of high acidity and heavy metal content. Currently, most strategies for AMD treatment merely focus on metal removal rather than metal recovery. However, bioelectrochemical system (BES) is a promising technology to simultaneously remove and recover metal ions from AMD. In this study, both cupric ion and cadmium ion in simulated AMD were effectively recovered by BES inoculated with a novel exoelectrogen, Pseudomonas sp. E8, that was first isolated from the anodic electroactive biofilm of a microbial fuel cell (MFC) in this study. Pseudomonas sp. E8 is a facultative anaerobic bacterium with a rod shape, 0.43–0.47 μm wide, and 1.10–1.30 μm long. Pseudomonas sp. E8 can agglomerate on the anode surface to form a biofilm in the single-chamber MFC using diluted Luria-Bertani (LB) medium as an energy substrate. A single-chamber MFC containing the electroactive Pseudomonas sp. E8 biofilms has a maximum output voltage of 191 mV and a maximum power density of 70.40 mW/m2, which is much higher than those obtained by most other exoelectrogenic strains in the genus of Pseudomonas. Almost all the Cu2+ (99.95% ± 0.09%) and Cd2+ (99.86% ± 0.04%) in simulated AMD were selectively recovered by a microbial fuel cell (MFC) and a microbial electrolysis cell (MEC). After the treatment with BES, the high concentrations of Cu2+(184.78 mg/L), Cd2+(132.25 mg/L), and total iron (49.87 mg/L) in simulated AMD were decreased to 0.02, 0.19, and 0 mg/L, respectively. Scanning electron micrograph (SEM), energy dispersive X-ray spectrometry (EDXS) and X-ray diffraction (XRD) analysis indicate that the Cu2+ and Cd2+ in simulated AMD were selectively recovered by microbial electrochemical reduction as Cu0 (together with trace amounts of Cu2O) or Cd0 on the cathode surface. Collectively, data suggest that Pseudomonas sp. E8 has great potential for AMD treatment and metal recovery.

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Comparative study on Cronobacter sakazakii and Pseudomonas otitidis isolated from septic tank wastewater in microbial fuel cell for bioelectricity generation

Cited by 48 publications

References 65 publications

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO₂ electrode

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO₂ electrode

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

Recovery of Metals from Acid Mine Drainage by Bioelectrochemical System Inoculated with a Novel Exoelectrogen, Pseudomonas sp. E8

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Comparative study on Cronobacter sakazakii and Pseudomonas otitidis isolated from septic tank wastewater in microbial fuel cell for bioelectricity generation

Cited by 48 publications

References 65 publications

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO2 electrode

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO2 electrode

A novel process of anodic water denitrification in an upflow tubular microbial fuel cell: effect of C/N ratio

Recovery of Metals from Acid Mine Drainage by Bioelectrochemical System Inoculated with a Novel Exoelectrogen, Pseudomonas sp. E8

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Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO₂ electrode

Electricity generation potential of sewage sludge in sediment microbial fuel cell using Ti–TiO₂ electrode