Comparative study on crude and partially purified laccase from Polyporus durus ATCC 26726 in the decolorization of textile dyes and wastewater treatment

Wehaidy, Hala R.; El-Hennawi, H. M.; Ahmed, Samia A.; Abdel–Naby, Mohamed A.

doi:10.4103/epj.epj_2_18

Cited by 16 publications

(4 citation statements)

References 2 publications

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“…Industrial applications get benefits from the highest laccase production in shortest time, whereas fungal species require a longer laccase production cycle [ 35 ]. Researchers also need a longer fermentation duration of 14 or 20 days to get the highest laccase level [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Agitation role (Dissolved Oxygen) in production of laccase from newly identified Ganoderma multistipitatum sp. nov. and its effect on mycelium morphology

Umar,

Abid,

Elshikh

et al. 2023

BMC Microbiol

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Background Agitation speed influenced the production rate of laccase. Orbital speed not only influenced the enzyme production, but was also effective to dissolve the oxygen during growth of mycelium, spores, and chlamydospores. Shear effects of speed greatly influenced the morphology of mycelium. Methods Ganoderma multistipitatum was identified by ITS marker. Phylogenetic tree was constructed for species identification. Qualitatively by plate method contained guaiacol indicator, while quantitatively by submerged fermentation and Central Composite Design applied on agitation parameter for maximum laccase potential of this species. The effects of agitation speed on mycelium morphology were observed under compound and scanning electron microscope. Results Statistical optimization of agitation conditions were performed by using response surface methodology to enhance the production of laccase from Ganoderma multistipitatum sp. nov. Maximum laccase yield (19.44 × 105 ± 0.28 U/L) was obtained at 150 rpm grown culture, which was higher than predicted value of laccase production (19.18 × 105 U/L) under aerobic conditions (150 rpm). The 150 rpm provided the continuous flush of oxygen. The DO (dissolved oxygen) was maximum (65%) for “27 h” incubation at 150 rpm during laccase synthesis. The statistical value of laccase production was minimum under anaerobic or nearly static condition of 50 rpm. The predicted (12.78 × 105 U/L) and obtained (12.82 × 105 U/L) yield was low at 50 rpm. Optimization of orbital shaking for aeration conditions were performed by the use of “Response Surface Methodology”. The submerged shaking flasks were utilized as a nutrients growth medium to maximize the production of laccase from G. multistipitatum. The minimum incubation time highly influenced the laccase yield from 7 to 15 days via utilization of less cost-effective medium under a promising and eco-friendly method. The morphological effects of rpm on mycelium were examined under compound and scanning electron microscopy. Higher rpm (200, 230) shear the mycelium, while 150 to 200 rpm exhibited smoother and highly dense branches of mycelia. Conclusion The shear forces of 200 rpm caused the damages of mycelium and cells autolysis with less laccase production. This study concluded that 150 rpm saved the life of mycelium and enhanced the production rate of enzymes.

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Section: Discussionmentioning

confidence: 99%

Agitation role (Dissolved Oxygen) in production of laccase from newly identified Ganoderma multistipitatum sp. nov. and its effect on mycelium morphology

Umar,

Abid,

Elshikh

et al. 2023

BMC Microbiol

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“…This was a logical hint to loss the laccase activity 50 . The maximum laccase quantity in a shorter period is advantageous in industrial applications, while fungal species require longer period for laccase production 51 . In the same way, researchers prefer 14 to 20 days for maximum laccase secretion from wood rotting fungi 52 .…”

Section: Discussionmentioning

confidence: 99%

Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship

Umar

Ahmed

2022

Sci Rep

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The aim of this work to study an efficient laccase producing fungus Ganoderma leucocontextum, which was identified by ITS regions of DNA and phylogenetic tree was constructed. This study showed the laccase first-time from G. leucocontextum by using medium containing guaiacol. The growth cultural (pH, temperature, incubation days, rpm) and nutritional (carbon and nitrogen sources) conditions were optimized, which enhanced the enzyme production up to 4.5-folds. Laccase production increased 855 U/L at 40 °C. The pH 5.0 was suitable for laccase secretion (2517 U/L) on the 7th day of incubation at 100 rpm (698.3 U/L). Glucose and sucrose were good carbon source to enhance the laccase synthesis. The 10 g/L beef (4671 U/L) and yeast extract (5776 U/L) were the best nitrogen source for laccase secretion from G. leucocontextum. The laccase was purified from the 80% ammonium sulphate precipitations of protein identified by nucleotides sequence. The molecular weight (65.0 kDa) of purified laccase was identified through SDS and native PAGE entitled as Glacc110. The Glacc110 was characterized under different parameters. It retained > 90% of its activity for 16 min incubation at 60 °C in acidic medium (pH 4.0). This enzyme exerted its optimal activity at pH 3.0 and temperature 70 °C with guaiacol substrate. The catalytic parameters Km and Vmax was 1.658 (mM) and 2.452 (mM/min), respectively. The thermo stability of the laccase produced by submerged fermentation of G. leucocontextum has potential for industrial and biotechnology applications. The results remarked the G. leucocontextum is a good source for laccase production.

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“…Currently, some genera and species found in the corticioid and poroid Funga of the REC-UEG are already considered as having potential for biotechnological processes. Trametes and Polyporus are largely used (or studied) for obtaining enzymes and degrading potential environmental pollutants (Cruz-Morató et al 2013, Olicón-Hernández et al 2017, Wehaidy et al 2018). In addition, previous studies focusing on biotechnological aspects of some species from the reserve have shown potential results (Leonardo-Silva et al 2018, Naves et al 2019.…”

Section: Discussionmentioning

confidence: 99%

“…The decomposition of woody substrates by these fungi is possible due to enzyme production that degrades the constituents of wood (Lundell et al 2010). For this reason, some species have been used in biotechnological processes to obtain new sources of bioproducts and in bioremediation activity (Salvachúa et al 2013, Olicón-Hernández et al 2017, Grassi et al 2018, Wehaidy et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Wood-inhabiting corticioid and poroid fungi (Basidiomycota) from Reserva Ecológica da Universidade Estadual de Goiás, a remnant of the Brazilian Cerrado

et al. 2022

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Corticioid and poroid fungi are traditional morphological groups composed of ligninolytic species. Due to their efficiency in wood decomposition process, many species have great ecological importance, especially in nutrient cycling, as well as for their biotechnological properties. Nevertheless, knowledge about these fungi is scarce in many phytogeographic regions of Brazil, as is the case of the Cerrado, since mycodiversity studies in this biome are mainly focused on areas of the São Paulo state. Here we present the taxonomic inventory of corticioid and poroid fungi from the Reserva Ecológica da Universidade Estadual de Goiás (REC-UEG), a Cerrado remnant in Anápolis, Goiás, Brazil. The area is covered by three typical Cerrado landscapes (cerrado stricto sensu, mesophilic forest, and gallery forest), widely explored for scientific and educational purposes by the academic and regional community which seeks to learn about and preserve its biodiversity. Exsiccates deposited in the fungarium of the Universidade Estadual de Goiás (HUEG-Fungi) were analyzed. They are the result of collections made over 20 years, in approximately 103 ha of the reserve. Samples were characterized macro and microscopically and identified based on specialized literature. 51 species were recognized, which are distributed in 33 genera, 15 families, and five orders. Species were most frequent in forested areas and among them, six are new occurrence records for the Cerrado, nine for the Midwest region, and nine for the Goiás state. These results contribute to increase the knowledge of these fungi in the Cerrado, as well as geographic distribution, and show the relevance of preserving the reserve for the regional Funga representativity.

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Comparative study on crude and partially purified laccase from Polyporus durus ATCC 26726 in the decolorization of textile dyes and wastewater treatment

Cited by 16 publications

References 2 publications

Agitation role (Dissolved Oxygen) in production of laccase from newly identified Ganoderma multistipitatum sp. nov. and its effect on mycelium morphology

Agitation role (Dissolved Oxygen) in production of laccase from newly identified Ganoderma multistipitatum sp. nov. and its effect on mycelium morphology

Optimization, purification and characterization of laccase from Ganoderma leucocontextum along with its phylogenetic relationship

Wood-inhabiting corticioid and poroid fungi (Basidiomycota) from Reserva Ecológica da Universidade Estadual de Goiás, a remnant of the Brazilian Cerrado

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