Comparative Study on Mechanistic Kinetic Modeling of the Enzymatic Synthesis of Galacto‐Oligosaccharides

Abstract

The galacto-oligosaccharide synthesis by b-galactosidase from Bacillus circulans is described mathematically using three kinetic models of different complexity -a simple power law approach, an extended Michaelis Menten kinetics and, in particular, an advanced, innovative mechanistic kinetic model for biocatalysis. Based on a detailed experimental study, a comprehensive reaction network is identified, and a reliable database provided. The evaluation of the kinetic models studied is performed by the Akaike information criterion, which allows the assessment of the model accuracy taking into account complexity and transferability.

Potential of Integrated Semi‐Continuous Enzymatic Synthesis and Filtration Processes for Efficiency Enhancement

“…2d. This includes studying the influence of individual parameters in combination with modeling for process optimization, based on the previous model approaches from [34].…”