Comparative Study on Semen Quality and Fertility of Red Chittagong Cattle, BCB1 and Munshiganj Bulls of Bangladesh

Abstract

The present study was conducted to analyze the fresh and post-thaw semen quality and fertility from native bulls of Red Chittagong Cattle (RCC), BLRI Cattle Breed 1 (BCB1), and Munshiganj Cattle of Bangladesh. One hundred and seventy-two ejaculates were collected by artificial vagina set and semen analysis was performed using Computer Assisted Sperm Analyzer (CASA) at Bangladesh Livestock Research Institute. Commercial extender (AndroMed) was used to dilute the fresh semen. After equilibration (4˚C for 4 hr), freezing was done using a programmable bio-freezer. Post-thawed semen was evaluated for sperm motility and kinematics. Cryopreserved semen straws were used for artificial insemination (AI) and determined the bull fertility based on 60 days non-return rate. Motility of the sperm differs significantly (p < 0.01) among the genotypes. Total motility was higher in Munshiganj bulls and static motility was higher in BCB1 bulls. However, the semen volume and sperm concentration did not vary significantly (p > 0.05) among the bulls but the highest concentration was found in Munshiganj bull (1669.60 ± 192.07 million/ml) followed by RCC (1648.70 ± 91.07 million/ml) and BCB1 bull (1481.60 ± 167.35 million/ml). Moreover, the highest bent tail (5.89 ± 0.75%), coiled tail (1.01% ± 0.22%) and distal mid-piece reflex (2.26% ± 0.28%) were observed in BCB1 followed by Munshiganj and RCC. Amplitude of lateral head displacement (ALH) was recorded higher in post-thaw than in fresh semen. Kinematics parameters of post-thaw semen decreased than fresh semen irrespective of genotypes. More number of doses/ejaculates can be produced from Munshiganj bull (394.34 ± 127.95) followed by RCC (349.01 ± 120.91

Morphometric features, seminal profile and diluters effect on post-thaw semen quality of Munshiganj cattle in Bangladesh

“…In the current study, average sperm concentration was found 1510.27 ± 844.07 million/ml which was similar with the findings in Munshiganj bull (1669.60 ± 192.07 million/ml) [ 48 ]and (1796 ± 122.29 million/ml) [ 56 ]. The present study findings was much higher than the report of [ 54 ] (907.39 ± 23.01 million/ml in RCC bull), and (1035.24 ± 279.79 to 1088.12 ± 297.14 million/ml) in Bali bull in Indonesia [ 49 ].…”

“…Semen quality can be affected by a wide range of genetic and environmental factors including bull age, collection interval, collection frequency, and season [ [45] , [46] , [47] ]. Regarding semen volume, present findings are in consistent with the findings 4.93 ± 0.15 ml in Munshiganj bull [ 48 ] and 4.88 ± 1.55 to 6.25 ± 1.43 ml/ejaculate in Bali bull in Indonesia [ 49 ]. On the other hand, 5.08–6.00 ml/ejaculation were reported for BLRI cattle breed-1 [ 50 ].…”

Age Effects on Semen Quality and Sperm Kinematics of Red Chittagong Cattle (RCC) Bull in Bangladesh

“…In swamp buffalo, there were significant differences in the age of buffalo on dose/ejaculates and 7 years bull produced more dose/ejaculate than 5 and 6 years of bull [21]. The quantity of frozen semen dose produced by the experimental bull in this experiment is within the standard range (349.01 dose/ejaculate) of mature RCC bull [20]. However, the total number of frozen semen production depends on management, freezing process, collection frequency, nutrition, and other factors.…”

“…The kinematic properties of fresh and frozen semen did not vary significantly among the different age groups of RCC bulls. Different kinematics properties of younger and older bulls are almost similar to the range of mature RCC bulls [20].…”

Addition of Tomato Juice as Additive in Diluent of Egg Yolk Citrate on the Quality of Pesisir Cattle Semen

“…The volume of semen of Pesisir cattle ranged from 2 ml to 4 ml. This result is lower than the semen of Munshiganj cattle which ranges up to 4.93 ml (Hossain et al, 2022), however it is higher than the other local cattle such as Bali cattle which has an average of 3.4 ml (Susilawati et al 2018). Low or high volume does not cause harm, but if the concentration is low, it will cause a limited number of spermatozoa in one ejaculate (Wafa et al, 2010).…”