Comparative study on the antibacterial activity of volatiles from sage (Salvia officinalis L.)

Abstract

Antibacterial activity of volatiles from sage against Gram-positive and Gram-negative bacteria from the ATCC collection was screened with the disk diffusion test. The essential oil and its fractions showed a significant antibacterial effect against S. aureus and B. subtilis. The minimum inhibitory concentrations were 1.25-2.5 μL/mL for S. aureus and 0.15-2.5 μL/mL for B. subtilis. The effect on S. aureus was bactericidal, while initial bactericidal effect on B. subtilis was impaired by the presence of a resistant fraction of the population, probably endospores. The results obtained with wild type and permeable strains of E. coli and S. typhimurium indicate that transport through the cell wall limits the antibacterial effect of sage volatiles

2-(chromon-3-yl)imidazole derivatives as potential antimicrobial agents: synthesis, biological evaluation and molecular docking studies

“…In vitro antibacterial studies of various compounds (4a-h and 6a-h) were carried out against Gram negative and Gram positive bacterial strains by disc diffusion assay [21]. The Gram positive strains used were Staphylococcus aureus (MTCC 96) and Bacillus subtilis (MTCC 2451), and Gram negative bacteria were Escherichia coli (MTCC 82) and Pseudomonas aeruginosa (MTCC 2642).…”

“…The antifungal activity of synthesized compounds against Aspergillus niger (MTCC 1344), Saccharomyces cerevisiae (MTCC 172), C. albicans (MTCC 3018) and Cryptococcus gastricus (MTCC 1715) was performed by disc diffusion method [21] by observing the zone of inhibition in comparison to the standard antifungal discs (fluconazole and griseofulvin). Test compounds were dissolved in DMSO to make a stock solution of 1 mg/ml.…”

“…All synthesized compounds (4a-h and 6a-h) were tested against four reference fungal strains such as S. cerevisiae (MTCC 172), Cryptococcus gastricus (MTCC 1715), Candida albicans (MTCC 3018) and A. niger (MTCC 1344), by disc diffusion method [21]. Minimum inhibitory concentration (MIC) in microgram per millilitre of compounds exhibiting activity (Table 4) was determined by using serial tube dilution method [22].…”

Molecular Mechanisms of Action of Antimutagens from Sage (Salvia officinalis) and Basil (Ocimum basilicum)

“…Furthermore, the latter is irreplaceable spice of many national cuisines. Numerous biological activities of different extracts of Salvia and Ocimum species have been described, including antimicrobial, anti-inflammatory, antioxidative, antidiarrheal, blood-sugar lowering, immunomodulatory, a nervous system stimulatory, spasmolytic, and cholinergic binding [29][30][31][32][33][34][35][36][37][38][39][40]. Several reports also indicate antigenotoxic and chemopreventive activities of different extracts from Salvia and Ocimum species [41][42][43][44][45].…”

Antioxidant, antimicrobial andantimutagenic potencial of the Myrtuscomunis L.

“…Naročito velika pažnja se posvećuje ispitivanju aromatičnih biljaka, jer se zna da mnoga etarska ulja ispoljavaju snažno antimikrobno dejstvo (Yamasaki et al, 1998;Tasoou et al, 1995;Sivropoulou et al, 1995;Marino et al, 2001;Friedman et al, 2002;Soliman i Badeaa, 2002;Yu-Chang Su et al, 2006;Mitić-Ćulafić et al, 2005;Božin et al, 2007;Mimica-Dukić i Božin, 2008 (Chamount i Leger, 1992;Pattnak et al, 1997;Stojanović et al, 2000;Friedman et al, 2002). Takođe, karvakrol, timol i pulegon su pokazali snažno antimikrobno dejstvo na širok spektar patogenih mikroorganizama (Simić et al, 2000;Ultee et al, 1998).…”