Comparative study on the mRNA expression of Pinus massoniana infected by Bursaphelenchus xylophilus

Xie, Wanfeng; Liang, Guanghong; Huang, Aizhen; Zhang, Feiping; Guo, Wenhui

doi:10.1007/s11676-018-0824-1

Cited by 15 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We attempted to isolate the viruses from two positive samples by inoculating them in CELC and CPE was noted within 3 d. Several researchers have attempted to conduct isolation trials of FAdV in CELC and been successful in virus adaptation ( Kataria et al., 1997 ; Barua and Rai, 2003 ). The FAdV was propagated in primary CELC in the current study, and the virus caused rounding, degeneration, and detachment of the infected cells from the monolayer, as observed by many other previous researchers ( Balamurugan et al., 2002 ; Soumyalekshmi et al., 2014 ; Xie et al., 2020 ). The pathogenesis of the isolated strains was demonstrated in one-day-old commercial broiler chickens via the oral route in the present study.…”

Section: Discussionsupporting

confidence: 75%

Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

Lebdah¹,

Alshaya²,

Jalal³

et al. 2022

Poultry Science

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 75%

Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

Lebdah¹,

Alshaya²,

Jalal³

et al. 2022

Poultry Science

View full text Add to dashboard Cite

“…The rate of expression was normalized to that of the reference gene. The relative fold differences in the expression of the BAX and BCL-2 genes were determined using the 2 −ΔΔCt method 72,73 . The bovine primers used in qRT-PCR are shown in Table 2.…”

Section: Cumulus-oocyte Complex Collection and In Vitro Embryo Producmentioning

confidence: 99%

Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised

Kowsar

Ronasi

Sadeghi

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Upon insemination, sperm cells are exposed to components of the female reproductive tract (FRT) fluids, such as urea and epidermal growth factor (EGF). It has been shown that both urea and EGF use EGF receptor signaling and produce reactive oxygen species (ROS) that are required at certain levels for sperm capacitation and acrosome reaction. We therefore hypothesized that during bovine sperm capacitation, a high level of urea and EGF could interfere with sperm function through overproduction of ROS. High-level urea (40 mg/dl urea is equal to 18.8 mg/dl of blood urea nitrogen) significantly increased ROS production and TUNEL-positive sperm (sperm DNA fragmentation, sDF) percentage, but decreased HOS test score, progressive motility, acrosome reaction and capacitation. The EGF reversed the negative effects of urea on all sperm parameters, with the exception of ROS production and DNA fragmentation, which were higher in urea-EGF-incubated sperm than in control-sperm. The developmental competence of oocytes inseminated with urea-EGF-incubated sperm was significantly reduced compared to the control. A close association of ROS production or sDF with 0-pronuclear and sperm non-capacitation rates was found in the network analysis. In conclusion, EGF enhanced urea-reduced sperm motility; however, it failed to reduce urea-increased sperm ROS or sDF levels and to enhance subsequent oocyte competence. The data suggests that any study to improve sperm quality should be followed by a follow-up assessment of the fertilization outcome.

show abstract

“…xylophilus. In agreement with this view, the downregulation of CC-NBS-LRR in vulnerable strains of P. massoniana is involved in the pathogenic pathways of nematode infestation [23]. Serine/arginine-rich (SR) proteins are the key players of alternative splicing, emerging as a critical co-transcriptional regulation for plants in response to environmental stresses [24].…”

Section: Plos Onementioning

confidence: 80%

Proteomic analysis of Masson pine with high resistance to pine wood nematodes

et al. 2022

View full text Add to dashboard Cite

Pine wilt disease is a dangerous pine disease globally. We used Masson pine (Pinus massoniana) clones, selected through traditional breeding and testing for 20 years, to study the molecular mechanism of their high resistance to pine wood nematodes (PWN,Bursaphelenchus xylophilus). Nine strains of seedlings of genetically stable Masson pine screened from different families with high resistance to PWN were used. The same number of sensitive clones were used as susceptible controls. Total proteins were extracted for tandem mass tag (TMT) quantitative proteomic analysis. The key proteins were verified by parallel reaction monitoring (PRM). A threshold of upregulation greater than 1.3-fold or downregulation greater than 0.3-fold was considered significant in highly resistant strains versus sensitive strains. A total of 3491 proteins were identified from the seedling tissues, among which 2783 proteins contained quantitative information. A total of 42 proteins were upregulated and 96 proteins were downregulated in the resistant strains. Functional enrichment analysis found significant differences in the proteins with pectin esterase activity or peroxidase activity. The proteins participating in salicylic acid metabolism, antioxidant stress reaction, polysaccharide degradation, glucose acid ester sheath lipid biosynthesis, and the sugar glycosaminoglycan degradation pathway were also changed significantly. The PRM results showed that pectin acetyl esterase, carbonic anhydrase, peroxidase, and chitinase were significantly downregulated, while aspartic protease was significantly upregulated, which was consistent with the proteomic data. These results suggest that Masson pine can degrade nematode-related proteins by increasing protease to inhibit their infestation, and can enhance the resistance of Masson pine to PWN by downregulating carbon metabolism to limit the carbon available to PWN or for involvement in cell wall components or tissue softening. Most of the downregulated proteins are supposed to act as an alternative mechanism for latter enhancement after pathogen attacks. The highly resistant Masson pine, very likely, harbors multiple pathways, both passive and active, to defend against PWN infestation.

show abstract

Comparative study on the mRNA expression of Pinus massoniana infected by Bursaphelenchus xylophilus

Cited by 15 publications

References 22 publications

Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

Molecular characterization of aviadenovirus serotypes and pathogenicity of the identified adenovirus in broiler chickens

Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised

Proteomic analysis of Masson pine with high resistance to pine wood nematodes

Contact Info

Product

Resources

About