Comparative Study on Two Antioxidant Flavanol-3-O-α-<i>L</i>- Rhamnosides in the Leaves of <i>Toona sinensis</i> (Chinese Toon) from Different Production Sites by HPLC-UV

Background:Toona sinensis (A. Juss.) Roemer is an endemic species of Toona genus native to Asian area. Its dried leaves are applied in the treatment of many diseases; however, few investigations have been reported for the quantitative analysis and comparison of major bioactive flavonol glycosides in the leaves harvested from various origins.Objective:To quantitatively analyze four major flavonol glycosides including rutinoside, quercetin-3-O-β-D-glucoside, quercetin-3-O-α-L-rhamnoside, and kaempferol-3-O-α-L-rhamnoside in the leaves from different production sites and classify them according to the content of these glycosides.Materials and Methods:A high-performance liquid chromatography-diode array detector (HPLC-DAD) method for their simultaneous determination was developed and validated for linearity, precision, accuracy, stability, and repeatability. Moreover, the method established was then employed to explore the difference in the content of these four glycosides in raw materials. Finally, a hierarchical clustering analysis was performed to classify 11 voucher specimens.Results:The separation was performed on a Waters XBridge Shield RP18 column (150 mm × 4.6 mm, 3.5 μm) kept at 35°C, and acetonitrile and H2O containing 0.30% trifluoroacetic acid as mobile phase was driven at 1.0 mL/min during the analysis. Ten microliters of solution were injected and 254 nm was selected to monitor the separation. A strong linear relationship between the peak area and concentration of four analytes was observed. And, the method was also validated to be repeatable, stable, precise, and accurate.Conclusion:An efficient and reliable HPLC-DAD method was established and applied in the assays for the samples from 11 origins successfully. Moreover, the content of those flavonol glycosides varied much among different batches, and the flavonoids could be considered as biomarkers to control the quality of Chinese Toon.SUMMARY Four major flavonol glycosides in the leaves of Toona sinensis were determined by HPLC-DAD and their contents were compared among various origins by HCA. Abbreviations used: HPLC-DAD: High-performance liquid chromatography-diode array detector, HCA: Hierarchical clustering analysis, MS: Mass spectrometry, RSD: Relative standard deviation.

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“…Finally, a hierarchical clustering analysis (HCA) was carried out to classify 11 voucher specimens. [ 31 32 33 34 ]…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis and comparison of four major flavonol glycosides in the leaves of Toona sinensis (A. Juss.) roemer (chinese toon) from various origins by high-performance liquid chromatography-diode array detector and hierarchical clustering analysis

et al. 2016

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Diversity of red, green and black cultivars of Chinese Toon [Toona sinensis (A. Juss.) Roem]: anthocyanins, flavonols and antioxidant activity

Wang

et al. 2020

Food Measure

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Establishment of a rapid method to quantify eight flavonol glycosides for quality assessment of red toon using UPLC

Shen

Pei-pei

et al. 2018

Acta Chromatographica

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Red Toon is a popular vegetable of favorable health benefits over Asia and Russia regions. In this study, isolation and identification of chemical constituents were performed to assess the quality of this functional food cultivated in various origins or harvested in different months. As a result, eight flavonol glycosides including rutin (I), myricitrin (II), quercetin-3-O-β-d-galactoranoside (III), quercetin-3-O-β-d-glucopyranose (IV), quercetin-3-O-α-l-arabinopyranoside (V), astragalin (VI), quercetin-3-O-α-l-rhamopyranoside (VII), and kaempferol-3-O-α-l-rhamopyranoside (VIII) were obtained. Among these, compounds III and V were isolated from Toona genus for the first time. Importantly, a rapid and convenient ultra-performance liquid chromatography (UPLC) method was developed to quantify the flavonol glycosides in Red Toon and validated for linearity, precision, stability, repeatability, and accuracy successively. In addition, it was found that total flavonoid glycosides (about 2.6%) in the food were kept at a higher level from April to June than other months of the year. Furthermore, their content in the Red Toon collected from ten different origins was also determined and compared, and the results suggested that the total flavonoid glycosides from Shandong Yantai were the highest, followed by Shandong Ximou, supporting a well-recognized viewpoint that Red Toon cultivated in Shandong Province, China, is considered genuine due to the best health benefits and flavor.

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Comparative Study on Two Antioxidant Flavanol-3-O-α-L- Rhamnosides in the Leaves of Toona sinensis (Chinese Toon) from Different Production Sites by HPLC-UV

Cited by 4 publications

References 12 publications

Quantitative analysis and comparison of four major flavonol glycosides in the leaves of Toona sinensis (A. Juss.) roemer (chinese toon) from various origins by high-performance liquid chromatography-diode array detector and hierarchical clustering analysis

Quantitative analysis and comparison of four major flavonol glycosides in the leaves of Toona sinensis (A. Juss.) roemer (chinese toon) from various origins by high-performance liquid chromatography-diode array detector and hierarchical clustering analysis

Diversity of red, green and black cultivars of Chinese Toon [Toona sinensis (A. Juss.) Roem]: anthocyanins, flavonols and antioxidant activity

Establishment of a rapid method to quantify eight flavonol glycosides for quality assessment of red toon using UPLC

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