Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs

Ghilotti, James; Sanz, José Luis Graupera; López‐García, Sergio; Guerrero‐Gironés, Julia; Pecci-Lloret, María Pilar; Lozano, Adrián; Llena, Carmen; Rodríguez‐Lozano, Francisco Javier; Forner, Leopoldo; Spagnuolo, Gianrico

doi:10.3390/ma13092189

Cited by 41 publications

(55 citation statements)

References 49 publications

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“…Besides, significant oxidative effects were not evidenced, especially with NeoMTA 2 and NeoMTA Plus. These results suggest that these materials did not exhibit an apparent cytotoxic effect after 48 h and 72 h. These findings are consistent with previous reports showing that NeoMTA Plus and Bio-C Repair elutes were not cytotoxic [28,34].…”

Section: Discussionsupporting

confidence: 92%

“…It has been described that Ca 2+ release stimulates hydroxyl apatite formation and release of alkaline phosphatase and bone morphogenetic protein 2, which are important in the mineralization process [27]. Both SEM-EDS and ICP-MS assays exhibited Ca 2+ release and calcium content in all materials, as previously described for NeoMTA Plus and Bio C-Repair [28,29]. Moreover, tantalum (Ta 5+ ) was detected both in NeoMTA 2 and NeoMTA Plus.…”

Section: Discussionsupporting

confidence: 70%

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Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

et al. 2021

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Objective The present study evaluated the biological effects and biomineralization potential of a new tantalum oxide (Ta2O5)–containing material designed for vital pulp therapy or perforation repair (NeoMTA 2), compared to NeoMTA Plus and Bio-C Repair. Material and methods Human dental pulp stem cells (hDPSCs) were exposed to different eluates from NeoMTA Plus, NeoMTA 2, and Bio-C Repair. Ion release from each material was determined using inductively coupled plasma-optical emission spectrometry (ICP-MS). The biological experiments performed were MTT assays, apoptosis/necrosis assays, adhesion assays, migration assays, morphology evaluation, and reactive oxygen species (ROS) production analysis. Biomineralization was assessed by Alizarin red S staining. Finally, osteo/odontogenic gene expression was determined by real-time quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. Results NeoMTA 2 displayed a significantly higher calcium release compared to the other materials (p < 0.05). When hDPSCs were cultured in presence of the different material eluates, all groups exhibited similar hDPSC viability and migration rates when compared to untreated cells. Substantial cell attachment and spreading were observed in all materials’ surfaces, without significant differences. hDPSCs treated with NeoMTA 2 displayed an upregulation of ALP, Col1A1, RUNX2 (p < 0.001), ON, and DSPP genes (p < 0.05), and showed the highest mineralization potential compared to other groups (p < 0.001). Finally, the more concentrated eluates from these materials, specially NeoMTA Plus and NeoMTA 2, promoted higher ROS production in hDPSCs compared to Bio-C Repair and control cells (p < 0.001), although these ROS levels did not result in increased cell death. Conclusions The new tantalum oxide (Ta2O5)–containing material shows an adequate cytocompatibility and the ability to promote biomineralization without using chemical osteogenic inducers, showing great potential as a new material for vital pulp therapy. Clinical relevance NeoMTA 2 seems to be a promising material for vital pulp therapy. Further studies considering its biocompatibility and biomineralization potential are necessary.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 70%

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

et al. 2021

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“…The sealing, antibacterial, and antifungal activities as well as the ability to either function as human tissues or encourage their regeneration [29] strengthen their promising application in medicine and dentistry [30,31]. Particularly, successful outcomes over time have been reported when bioactive calcium silicate-based cements were applied in vital pulp therapy, endodontic restoration, endodontic sealing, and regenerative endodontics [5,28,29,32].…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases

Bossù

Mancini

Bruni

et al. 2021

Biology

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Calcium silicate-based cements have reached excellent levels of performance in endodontics, providing predictable and successful results. To better assess the properties of these bioactive materials, the present study aimed to compare the biocompatibility and antibiofilm properties of ProRoot MTA and Biodentine. Human osteogenic sarcoma (Saos-2) cells were cultured on ProRoot MTA and Biodentine samples or in the presence of both cement extracts. Cell viability assay, measurement of reactive oxygen species (ROS), immunofluorescence analysis, as well as morphological evaluations were conducted. Moreover, Streptococcus mutans was used to assess the biofilm forming ability on ProRoot MTA and Biodentine disks. Finally, both cements were applied in vivo to treat immature permanent teeth affected by reversible pulpitis. Results: Cell viability assay demonstrated that Saos-2 cells had a dose- and time-dependent cytotoxicity to both analyzed cements, although cells exposed to ProRoot MTA showed a better cell vitality than those exposed to Biodentine (p < 0.001). Both cements demonstrated ROS production while this was greater in the case of Biodentine than ProRoot MTA (p < 0.001). Immunofluorescence images of the cytoskeleton and focal adhesions showed no differences in Saos-2 cells grown in the presence of ProRoot MTA eluate; whereas in the Biodentine groups, cells showed a morphology and focal adhesions more similar to that of the control sample, as the eluate concentration decreased. Morphological analysis revealed that Saos-2 cells were more flattened and exhibited better spreading when attached to ProRoot MTA disks than to Biodentine ones. The antibiofilm properties showed a time-dependent powerful inhibition of S. mutans superficial colonization and an antibiofilm effect of both cements. Clinically, complete root formation of the treated elements was achieved using the two studied cements, showing stable results over time. ProRoot MTA and Biodentine was demonstrated to be biocompatible and to possess antibiofilm properties. Their clinical application in vital pulp therapy provided successful outcomes after 2 years of follow-up.

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“…In stem cells from the periodontal ligament, Bio-C Repair and Bio-C Sealer had similar pHs, but Bio-C Repair exhibited greater cell viability and cell adhesion, indicating its bioactivity. This cement showed excellent cytocompatibility, similar to Biodentine and ProRoot MTA when used with a chemical technique and ultrastructural morphology on human dental pulp cells (9).…”

Section: Introductionmentioning

confidence: 88%

“…The distribution and particle size of tricalcium silicate powders affect handling and setting properties, since smaller particles can penetrate dentin tubules, leading to quicker hydration (7,8). Also, zirconia oxide, used as a radioopacifier, was detected at high levels in Bio-C Repair and Biodentine cement when compared with ProRoot MTA (9). This component is associated with the prevention of tooth discoloration (10).…”

Section: Introductionmentioning

confidence: 99%

Bio-C Repair - A New Bioceramic Material for Root Perforation Management: Two Case Reports

et al. 2021

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These case reports aimed to describe the management of lateral perforation in the middle cervical third of the root in two maxillary incisors with pulp canal calcification using Bio-C Repair, with safe and viable clinical treatment strategies. Digital radiographic exams were obtained with different angles and analyzed using different filters. Cone-beam computed tomography (CBCT) images were requested to show the actual position of the canal, location of the perforation, and guide the strategic planning of the case. Subsequently, cavity access was prepared with the aid of dental operating microscopy. After perforation was identified, granulation tissue was removed and the original canal was identified and then dressed with calcium hydroxide. In the second visit, the perforation was filled with Bio-C Repair and the canal system filled with gutta-percha points and a root canal sealer (Bio-C Sealer). The teeth were restored with glass fiber post, 4 mm beyond the perforation level, and provisory crowns. Both teeth treated as described above were functional and asymptomatic with a 1-year clinical and radiographic assessment. The Bio-C Repair is suggested as a new cement option for the management of lateral canal perforations, with effective results as observed after a one-year follow-up.

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Comparative Surface Morphology, Chemical Composition, and Cytocompatibility of Bio-C Repair, Biodentine, and ProRoot MTA on hDPCs

Cited by 41 publications

References 49 publications

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases

Bio-C Repair - A New Bioceramic Material for Root Perforation Management: Two Case Reports

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