Comparative transcriptome analysis of defense response of rice to Nilaparvata lugens and Chilo suppressalis infestation

Li, Hanpeng; Zhou, Zaihui; Hua, Hongxia; Ma, Weihua

doi:10.1016/j.ijbiomac.2020.09.105

Cited by 18 publications

(24 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the GO term and KEGG pathway enrichment analysis, P ‐values <0.05 were set as the standard for significant enrichment of DEGs. The heat map of DEGs was generated using EXCEL (2016) 22 …”

Section: Methodsmentioning

confidence: 99%

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Pesticides are extensively applied globally. Pesticide residues induce calamitous effects on the environment and untargeted organisms. Public concerns for the safety of freshwater organisms and the challenges posed by aquatic contaminants remain high. In the present study, the acute toxicity of avermectins (AVMs) to the crayfish, Procambarus clarkii was evaluated. We also evaluated the potential effects of AVM on the biochemical and transcriptomic status of the hepatopancreas and gastrointestinal tract in P. clarkii. RESULTS:The 24, 48, 72, 96 h median lethal concentrations (LC 50 ) of AVM on crayfish were 2.626, 1.162, 0.723, 0.566 mg L −1 , respectively. The crayfish were then exposed to 0.65 mg L −1 of AVM for 96 h. AVM significantly altered biochemical parameters including AChE and CAT activities in the hepatopancreas, and AChE, SOD and Na + -K + -ATPase activities in the gastrointestinal tract at several time points. Furthermore, transcriptomic analysis identified 953 and 1851 differentially-expressed genes (DEGs) in the hepatopancreas and gastrointestinal tract, respectively. KEGG enrichment showed that the gene expression profiles of the hepatopancreas and gastrointestinal tract were distinct from each other. The DEGs in the hepatopancreas were mostly enriched with stress-response pathways, while the majority of the DEGs in the gastrointestinal tract belonged to metabolism-related pathways.CONCLUSION: We demonstrated that the AVM induced acute toxicity, oxidative stress, osmoregulation disturbance, neurotoxicity and transcriptome imbalance in crayfish. These findings unraveled the detrimental effects of AVMs exposure on crayfish.

show abstract

Section: Methodsmentioning

confidence: 99%

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Zhu

Guan

Liao

et al. 2022

Pest Management Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Generally, rice plants infested by SSB larvae displayed higher anti-herbivore responses against insects (Li et al 2020 ; Liu et al 2021 ). RNAi-mediated silencing of OsAOS2 involved in JA biosynthesis significantly reduced the resistance of rice plants to SSB infestation (Mei et al 2006 ).…”

Section: Discussionmentioning

confidence: 99%

Rice Defense Responses Orchestrated by Oral Bacteria of the Rice Striped Stem Borer, Chilo suppressalis

Xue

Guo

et al. 2023

Rice

View full text Add to dashboard Cite

Plant defenses in response to chewing insects are generally regulated by jasmonic acid (JA) signaling pathway, whereas salicylic acid (SA) signaling is mainly involved in plant defense against biotrophic pathogens and piercing-sucking insects. Previous studies showed that both JA- and SA-related defenses in rice plants were triggered by the infestation of the rice striped stem borer (SSB, Chilo suppressalis), a destructive pest causing severe damage to rice production. Herbivore-associated microbes play an important role in modulating plant–insect interaction, and thus we speculate that the SSB symbiotic microbes acting as a hidden player may cause this anomalous result. The antibiotics (AB) treatment significantly depressed the performance of field-collected SSB larvae on rice plants, and reduced the quantities of bacteria around the wounds of rice stems compared to non-AB treatment. In response to mechanical wounding and oral secretions (OS) collected from non-AB treated larvae, rice plants exhibited lower levels of JA-regulated defenses, but higher levels of SA-regulated defenses compared to the treatment of OS from AB-treated larvae determined by using a combination of biochemical and molecular methods. Among seven culturable bacteria isolated from the OS of SSB larvae, Enterobacter and Acinetobacter contributed to the suppression of JA signaling-related defenses in rice plants, and axenic larvae reinoculated with these two strains displayed better performance on rice plants. Our findings demonstrate that SSB larvae exploit oral secreted bacteria to interfere with plant anti-herbivore defense and avoid fully activating the JA-regulated antiherbivore defenses of rice plants.

show abstract

“…The brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is known as a piercing and sucking pest that sucks the juice from the phloem of rice plants, Oryza sativa L. (Poaceae) (Liu et al, 2020; Li et al, 2020). Moreover, N. lugens not only damages rice directly by sucking stems, but also indirectly by vectoring several viruses, which cause serious losses in rice yield (Wu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Sublethal and transgenerational effects of a potential plant‐derived insecticide, β‐asarone, on population fitness of brown planthopper, Nilaparvata lugens

Wen

et al. 2022

Entomologia Exp Applicata

View full text Add to dashboard Cite

Many essential oils have excellent insecticidal activity and have the potential to be developed as alternatives to chemical insecticides for pest control. Previously, we showed that β‐asarone, a major constituent of the essential oil derived from Acorus calamus L. (Acoraceae), has strong pesticidal activity against brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), the most notorious rice pest in Asia. Here, we report the first evaluation of the sublethal and transgenerational effects of β‐asarone on N. lugens. β‐Asarone significantly decreased female longevity, male longevity, fecundity, and hatchability of F0 generation individuals exposed to the LD30 and LD45 of β‐asarone relative to the acetone control. Moreover, compared with acetone, exposure to LD30 and LD45 of β‐asarone significantly shortened the duration of the egg stage, developmental duration of first instars, and female longevity of F1 generation individuals. Furthermore, the intrinsic rate of increase (r), finite rate of increase (λ), and net reproductive rates (R0) of insects treated with LD30 and LD45 of β‐asarone were significantly lower than those of insects treated with acetone. Compared with acetone, the fecundity and hatchability of F1 generation individuals were significantly decreased after exposure to β‐asarone at LD30 and LD45. These findings indicate the negative effects of sublethal doses of β‐asarone on N. lugens and provide novel information on the potential use of β‐asarone as a substitute chemical pesticide.

show abstract

Comparative transcriptome analysis of defense response of rice to Nilaparvata lugens and Chilo suppressalis infestation

Cited by 18 publications

References 61 publications

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Acute toxicity, biochemical and transcriptomic analysis of Procambarus clarkii exposed to avermectin

Rice Defense Responses Orchestrated by Oral Bacteria of the Rice Striped Stem Borer, Chilo suppressalis

Sublethal and transgenerational effects of a potential plant‐derived insecticide, β‐asarone, on population fitness of brown planthopper, Nilaparvata lugens

Contact Info

Product

Resources

About