Comparative transcriptome analysis of genes involved in anthocyanin biosynthesis in the pink-white and red fruits of Chinese bayberry (Morella rubra)

Lin, Qihua; Zhong, Qing; Zhang, Zehuang

doi:10.1016/j.scienta.2019.02.061

Cited by 17 publications

(14 citation statements)

References 33 publications

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“…It is known that variations in color intensity can be attributed to differences in the expression of structural or regulatory genes. In pink–white Chinese bayberry fruit, LDOX and UFGT expression was 70% and 90% lower than that of the red fruit at the ripening stage [ 32 ]. Previous studies reported UFGT as the key gene of anthocyanin biosynthesis in sweet cherry [ 30 ], mangosteen [ 57 ], and litchi [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…PAL , CHS , CHI , DFR and LDOX gene expression was up-regulated in the red “Yunhong-1” pear variety compared to the white “Zaobaimi” variety [ 31 ]. Likewise, Lin et al [ 32 ] suggested that the pink–white mutant of Chinese bayberry was due to a reduction in the expression of key biosynthesis genes in the later stages of development. Moreover, their expression could be influenced by abiotic factors, such as temperature [ 33 ], light, [ 34 ] and hormones [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

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Expression of Structural Flavonoid Biosynthesis Genes in Dark-Blue and White Myrtle Berries (Myrtus communis L.)

Medda

Sanchez-Ballesta

Romero

et al. 2021

Plants

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Within the myrtle (Myrtus communis L.) species, different genotypes may produce dark-blue berries or white berries depending on the peel color upon ripening. One dark-blue cultivar and one white myrtle cultivar were used to study the molecular mechanisms underlying flavonoid biosynthesis. The relative expression levels of common (PAL, CHS, CHI, DFR and LDOX) and specific (FLS, ANR, LAR and UFGT) flavonoid genes were analyzed during fruit development by means of quantitative real-time polymerase chain reaction (RT-qPCR). Moreover, the anthocyanin content was determined, and it showed an increase with the ripening of the berries of the dark-blue cultivar. The results showed an increased transcript abundance of PAL, CHI, DFR, LDOX and UFGT gene expression in the dark-blue cultivar compared to the white one, as well as a strong positive correlation between the changes in gene expression and anthocyanin accumulation. The transcript levels of UFGT showed sharp increases at 150 and 180 days after full blooming (DAF) in the dark-blue cultivar, which corresponded with anthocyanin accumulation. However, ripening seemed to modulate the expression of genes implicated in flavonols (i.e., FLS) and flavan-3-ols (i.e., LAR and ANR) in different manners. However, whereas FLS transcript accumulation increased at the end of the ripening period in the dark-blue cultivar, LAR and ANR gene expression decreased in both cultivars.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of Structural Flavonoid Biosynthesis Genes in Dark-Blue and White Myrtle Berries (Myrtus communis L.)

Medda

Sanchez-Ballesta

Romero

et al. 2021

Plants

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“…OVERVIEW. The biological regulation of flower color has attracted much attention in the study of ornamental plants (Lin et al, 2019). Anthocyanin is known to contribute to plant coloration (Rusishvili et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Mechanism Underlying Color Variation in Calla Lily Spathes Based on Transcriptomic Analysis

Fang

Lei

et al. 2021

J. Amer. Soc. Hort. Sci.

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The calla lily (Zantedeschia hybrida) is a valued ornamental plant due to its unique shape and color variations. To determine the mechanisms responsible for color development in the calla lily spathe, we conducted a comparative transcriptomic analysis of the spathes of the black [Black Girl (B)], pink [Romantic (P)], and white [Ventura (W)] cultivars. The gene expression patterns in six spathe colors, including the preceding three colors as well as the amaranth [Promise (N)], red [Figo (F)], and yellow [Sun Club (Y)] cultivars were analyzed by real-time quantitative polymerase chain reaction (PCR). Transcriptomic analysis identified 25,165 differentially expressed genes. The transcription abundance and expression level of genes annotated as anthocyanidin reductase (ANR1, ANR2), basic-helix-loop-helix (bHLH1), and glutathione S-transferases (GST1) were significantly upregulated in B, and the expression of anthocyanidin synthase (ANS) was highest in B except for N. However, chalcone isomerase (CHI2) and dihydroflavonol 4-reductase (DFR1, DFR2) were expressed at significantly lower levels in P, W, and Y. Correlation analysis revealed that bHLH1 might act as a positive regulator of ANS expression, promoting anthocyanin synthesis. Moreover, GST1-encoded proteins may be related to the accumulation and transport of both anthocyanin and procyanidin in the calla lily spathe. It is speculated that the formation of the black spathe is related to the accumulation of anthocyanins and procyanidins. However, the low expression of CHI2, DFR1, and DFR2 may result in the inhibition of anthocyanin synthesis, which may lead to lightening of the spathe color. This preliminary study revealed the mechanism responsible for calla lily spathe color, identifying the key genes involved, thus providing effective gene resources and a theoretical basis for flower color molecular breeding.

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“…Based on the sequencing results, 99,319 unigenes were generated, which were identical to the 119,701 unigenes of Chinese bayberry (Lin, Zhong & Zhang, 2019) and 82,036 unigenes of litchi (Lu et al, 2014). 51,642 (52.00%) unigenes sequences were annotated, which enriched the Carambola sequence database as well as provided basic data for subsequent research.…”

Section: Transcriptome Sequencing Enriched Carambola Sequence Informationmentioning

confidence: 99%

Identification and functional analysis of SWEET gene family in Averrhoa carambola L. fruits during ripening

Lin

Zhong

Zhang

2021

PeerJ

Self Cite

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Sugar Will Eventually be Exported Transporters (SWEETs), a type of sugar efflux transporters, have been extensively researched upon due to their role in phloem loading for distant sugar transport, fruit development, and stress regulation, etc. Several plant species are known to possess the SWEET genes; however, little is known about their presence in Averrhoa Carambola L. (Oxalidaceae), an evergreen fruit crop (star fruit) in tropical and subtropical regions of Southeast Asia. In this study, we established an Averrhoa Carambola L. unigenes library from fruits of ‘XianMiyangtao’ (XM) by RNA sequencing (RNA-seq). A total of 99,319 unigenes, each longer than 200 bp with a total length was 72.00 Mb, were identified. A total of 51,642 unigenes (52.00%) were annotated. Additionally, 10 AcSWEET genes from the Averrhoa Carambola L. unigenes library were identified and classified, followed by a comprehensive analysis of their structures and conserved motif compositions, and evolutionary relationships. Moreover, the expression patterns of AcSWEETs in ‘XM’ cultivars during fruit ripening were confirmed using quantitative real-time PCR (qRT-PCR), combined with the soluble sugar and titratable acids content during ripening, showed that AcSWEET2a/2b and AcSWEET16b might participate in sugar transport during fruit ripening. This work presents a general profile of the AcSWEET gene family in Averrhoa Carambola L., which can be used to perform further studies on elucidating the functional roles of AcSWEET genes.

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Comparative transcriptome analysis of genes involved in anthocyanin biosynthesis in the pink-white and red fruits of Chinese bayberry (Morella rubra)

Cited by 17 publications

References 33 publications

Expression of Structural Flavonoid Biosynthesis Genes in Dark-Blue and White Myrtle Berries (Myrtus communis L.)

Expression of Structural Flavonoid Biosynthesis Genes in Dark-Blue and White Myrtle Berries (Myrtus communis L.)

Mechanism Underlying Color Variation in Calla Lily Spathes Based on Transcriptomic Analysis

Identification and functional analysis of SWEET gene family in Averrhoa carambola L. fruits during ripening

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