Comparative Transcriptome Analysis of Head Kidney of Aeromonas hydrophila-infected Hypoxia-tolerant and Normal Large Yellow Croaker

The identification of toll-like receptor genes in large yellow croaker (Larimichthys crocea): provides insights into its environmental adaptation to biological and abiotic stresses

“…Large yellow croaker (Larimichthys crocea), the native species of East Asia, has the highest aquaculture yield among marine fish in China (257,683 tons in 2022) (MOA, 2023). However, the intensive net-cage inshore cultivation model, the degrading farming environment, outbreaks of various abiotic and biotic stressors like hypoxia and Aeromonas hydrophila infection, and other factors, had caused significant economic losses to the aquaculture industry (Ding et al, 2022;Zhang et al, 2022). Many previous studies had confirmed that Toll-like receptor genes played a vital role in innate immunity (Ni et al, 2022;Yao et al, 2023;Gao et al, 2024), however, research on their role in environmental adaptability was still in its early stages.…”

“…On the other hand, the breeding of L.crocea was threatened by bacterial diseases (Mu et al, 2018). Previous studies had shown that the diseased L. crocea had suffered a variety of severe illness symptoms after Gram-negative bacteria A. hydrophila infection (Chen et al, 2010;Mu et al, 2010;Zhang et al, 2022), as well as the verification of TLRs differential expression following an A. hydrophila challenge in common carp (Gong et al, 2017). Therefore, in order to allow large yellow croak culture population, exploit a better ecological opportunity under the stress of environmental factors such as hypoxia and A. hydrophila infection, researchers were committed to exploring the molecular mechanism of resistance regulation of TLR genes.…”

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“…Large yellow croaker (Larimichthys crocea), the native species of East Asia, has the highest aquaculture yield among marine fish in China (257,683 tons in 2022) (MOA, 2023). However, the intensive net-cage inshore cultivation model, the degrading farming environment, outbreaks of various abiotic and biotic stressors like hypoxia and Aeromonas hydrophila infection, and other factors, had caused significant economic losses to the aquaculture industry (Ding et al, 2022;Zhang et al, 2022). Many previous studies had confirmed that Toll-like receptor genes played a vital role in innate immunity (Ni et al, 2022;Yao et al, 2023;Gao et al, 2024), however, research on their role in environmental adaptability was still in its early stages.…”

“…On the other hand, the breeding of L.crocea was threatened by bacterial diseases (Mu et al, 2018). Previous studies had shown that the diseased L. crocea had suffered a variety of severe illness symptoms after Gram-negative bacteria A. hydrophila infection (Chen et al, 2010;Mu et al, 2010;Zhang et al, 2022), as well as the verification of TLRs differential expression following an A. hydrophila challenge in common carp (Gong et al, 2017). Therefore, in order to allow large yellow croak culture population, exploit a better ecological opportunity under the stress of environmental factors such as hypoxia and A. hydrophila infection, researchers were committed to exploring the molecular mechanism of resistance regulation of TLR genes.…”