Comparative transcriptome analysis of pollen and anther wall reveals novel insights into the regulatory mechanisms underlying anther wall development and its dehiscence in rice

Hong, Woo-Jong; Lee, Su Kyoung; Kim, Seok-Hui; Kim, Yujin; Moon, Sunok; Kim, Eui–Jung; Silva, Jeniffer; Jung, Ki‐Hong

doi:10.1007/s00299-022-02852-3

Cited by 3 publications

(2 citation statements)

References 58 publications

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“…We used a RNeasy Plant Mini Kit from Qiagen to extract RNA from samples and then a TruSeq Stranded mRNA LT Sample Prep Kit for library construction. The libraries were sequenced on an Illumina platform (Illumina NovaSeq 6000) [76], with two biological replicates for each sample. Raw expression datasets for leaf, shoot, root, anther, pollen, seed, and callus tissues were downloaded from NCBI SRA (http://ncbi.nlm.nih.gov/sra/, accessed on 28 December 2022).…”

Section: Rna-seq Analysis and Data Collectionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Collar Region-Preferential Genes in Rice

Hong

Lee

et al. 2023

Plants

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The collar region plays a crucial role in leaf angle formation and plant architecture, which is important for improving crop yield given the challenges of diminishing arable land and changing environmental conditions. To determine collar region-preferential genes (CRPGs) affecting plant architecture and crop yield, we conducted genome-wide transcriptomic analysis. By integrating our RNA sequencing data with public rice anatomical expression data, we identified 657 CRPGs. Verification involved testing six randomly selected CRPGs, all of which exhibited collar-preferential expression. The functional significance of CRPGs was assessed via Gene Ontology enrichment analysis, utilizing MapMan and KEGG, and literature analysis provided additional information for characterized CRPGs. Our findings revealed links between manipulating leaf angle and phytohormone-related pathways and stress responses. Moreover, based on the CRPGs, five transcription factors downstream of the liguleless 1 (LG1) gene were identified. Overall, the identified CRPGs provide potential targets for further research and breeding applications aimed at improving crop productivity by manipulating leaf architecture.

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Section: Rna-seq Analysis and Data Collectionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Collar Region-Preferential Genes in Rice

Hong

Lee

et al. 2023

Plants

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“…Spikelet fertility of rice is associated with pollination and fertilization, which include four main stages: (i) anther dehiscence, which proceeds through the sequential processes of thickening of the inner wall of the locule, swelling of pollen grains by water absorption, rupturing of the septum, and dehydration and cracking of the stomium ( Hong et al, 2022 ); (ii) pollen dispersal, through which pollen grains are released from the anther pores ( Fahad et al, 2015 ), with the amount of pollen grains released depending on the size and shape of the anther pores and the viscosity of the pollen grains ( Santiago and Sharkey, 2019 ); (iii) pollen transport, in which pollen grains are dispersed and deposited onto the stigmas, and the pollen counts on the stigma depend on the distance and the relative positions between anther pores and the stigma ( Liu S. J. et al, 2015 ), as well as the microclimate of the canopy ( Yoshimoto et al, 2020 ); and (iv) pollen germination, pollen tube elongation, and embryo sac fertilization ( Matsui et al, 2021 ).…”

Section: The Pollination Barrier Is the Key Issue In Reduced Spikelet...mentioning

confidence: 99%

Potential roles of stigma exsertion on spikelet fertility in rice (Oryza sativa L.) under heat stress

2022

Front. Plant Sci.

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Heat stress during the flowering stage induces declining spikelet fertility in rice plants, which is primarily attributed to poor pollination manifesting as insufficient pollen deposited on the stigma. Plant pollination is associated with anther dehiscence, pollen dispersal characteristics, and stigma morphology. The mechanisms underlying the responses of spikelet fertility to heat stress have been clarified in depth in terms of the morphological and behavioral characteristics of the male reproductive organs in rice. However, the roles of female reproductive organs, especially the stigma, on spikelet fertility under heat conditions are unclear. The present study reviews the superiority of stigma exsertion on pollen receptivity under heat during the flowering stage and discusses the variations in the effects of exserted stigma on alleviating injury under asymmetric heat (high daytime and high nighttime temperatures). The pollination advantages of exserted stigmas seem to be realized more under high nighttime temperatures than under high daytime temperatures. It is speculated that high stigma exsertion is beneficial to spikelet fertility under high nighttime temperatures but detrimental under high daytime temperatures. To cope with global warming, more attention should be given to rice stigma exsertion, which can be manipulated through QTL pyramiding and exogenous hormone application and has application potential to develop heat-tolerant rice varieties or innovate rice heat-resistant cultivation techniques, especially under high nighttime temperatures.

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Floral Developmental Morphology and Biochemical Characteristics of Male Sterile Mutants of Lagerstroemia indica

Deng,

Lu,

et al. 2024

Plants

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Male sterility is a common phenomenon in higher plants and often plays an important role in the selection of superior offspring. ‘Xiang Yun’ is a mutant of Lagerstroemia indica that does not bear fruit after flowering, and its flowering period is significantly longer than that of normal L. indica. To explore the timing and molecular mechanisms of sterility in ‘Xiang Yun’, this study determined the period of sterility through anatomical observation and compared the content of nutrients and the activity of antioxidative enzymes at different stages of flower development. Finally, sequence alignment and qPCR were used to analyze the differences in pollen development genes between ‘Xiang Yun’ and ‘Hong Ye’. The results showed that the anthers of ‘Xiang Yun’ dispersed pollen normally, but the pollen grains could not germinate normally. Observations with scanning electron microscopy revealed that the pollen grains were uneven in size and shriveled in shape. Further observation of anther sections found that abnormal development of the microspores began at the S2 stage, with the callose wall between microspores of ‘Xiang Yun’ being thicker than that of ‘Hong Ye’. In addition, during the flower development of ‘Xiang Yun’, the contents of soluble sugar, soluble protein, free proline, and triglycerides were deficient to varying degrees, and the activities of POD, SOD, and MDA were lower. Sequence alignment and qPCR showed that there were several mutations in EFD1, TPD1, and DEX1 of ‘Xiang Yun’ compared with ‘Hong Ye’, and the expression levels of these genes were abnormally elevated in the later stages of development. Our results clarified the timing and phenotype of male sterility in ‘Xiang Yun’. This provides solid and valuable information for further research on the molecular mechanism of sterility in ‘Xiang Yun’ and the genetic breeding of crape myrtle.

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Comparative transcriptome analysis of pollen and anther wall reveals novel insights into the regulatory mechanisms underlying anther wall development and its dehiscence in rice

Cited by 3 publications

References 58 publications

Genome-Wide Identification and Analysis of Collar Region-Preferential Genes in Rice

Genome-Wide Identification and Analysis of Collar Region-Preferential Genes in Rice

Potential roles of stigma exsertion on spikelet fertility in rice (Oryza sativa L.) under heat stress

Floral Developmental Morphology and Biochemical Characteristics of Male Sterile Mutants of Lagerstroemia indica

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