Comparative transcriptome and gut microbiota analysis of Exopalaemon carinicauda with different growth rates from a full-sib family

Xing, Chaofan; Xiong, Jiayao; Xie, Shumin; Guo, Hanxuan; Hua, Songsong; Yao, Yajie; Zhu, Jiawei; Yan, Binlun; Shen, Xin; Gao, Huan; Wang, Panpan

doi:10.1016/j.aqrep.2023.101580

Cited by 2 publications

(2 citation statements)

References 49 publications

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“…Wang et al [44] found that the expression levels of the TnT and TnC genes of E. carinicauda were significantly upregulated in larger individuals compared with smaller individuals. In our previous study about E. carinicauda, we found that TnT and TnC were significantly upregulated in the fast-growing group [45]. These data also suggest that different subunits of troponin do regulate the growth of crustaceans, but the interactions between the different subunits need to be further explored.…”

Section: Discussionmentioning

confidence: 67%

Muscle Transcriptome Analysis Reveals Molecular Mechanisms of Superior Growth Performance in Kuruma Shrimp, Marsupenaeus japonicus

Wang¹,

Yu²,

Li³

et al. 2023

Fishes

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During the cultivation of Marsupenaeus japonicus, there are often obvious differences in the growth within the same family under the same food, water quality, and environment, which greatly affects cultivation efficiency. To explore the molecular mechanism of this growth difference, this study used RNA-seq technology to compare the transcriptomes of M. japonicus individuals with significant growth differences from the same family. A total of 1375 differentially expressed genes were identified, of which 1109 were upregulated and 266 were downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed on the differentially expressed genes, and the results showed that growth-related processes, including chitin metabolism, chitin-binding amino sugar metabolism, and antioxidant processes, including response to oxidative stress, peroxidase activity, antioxidant activity, and peroxidase activity, showed significant differences between the large-size and small-size groups. The expression levels of some differentially expressed genes, such as cuticular protein, low-density lipoprotein receptor, ecdysteroid kinase, myosin heavy chain, and apoptosis inhibitor, were verified by quantitative PCR experiments. One cuticle gene was annotated, and phylogenetic analysis showed that this sequence clustered with the penaeid cuticle genes. This study provides valuable data and a scientific basis for understanding the mechanism of growth differences in M. japonicus at the molecular-genetic level.

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Section: Discussionmentioning

confidence: 67%

Muscle Transcriptome Analysis Reveals Molecular Mechanisms of Superior Growth Performance in Kuruma Shrimp, Marsupenaeus japonicus

Wang¹,

Yu²,

Li³

et al. 2023

Fishes

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“…Exopalaemon carinicauda , also known as the ridgetail white prawn, is distributed mainly on the coast of mainland China and the west coast of the Korean Peninsula [ 23 , 24 ]. E. carinicauda has the advantages of a strong reproductive ability, a short growth cycle, and a long breeding season, so it represents an important economic breeding variety in China, the annual production of which exceeds 50,000 tons, and the output value exceeds USD 420 million [ 25 , 26 , 27 , 28 ]. Like most other crustaceans, E. carinicauda has the powerful ability to regenerate severed limbs.…”

Section: Introductionmentioning

confidence: 99%

Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda

Xing,

Wang,

Chen

et al. 2024

Animals

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With the increase in breeding density of Exopalaemon carinicauda, appendage breakage may occur, which seriously affects survival and economic benefits. To study the limb regeneration process of E. carinicauda, we induced autotomy of the pereopods. After a period of time, wound swelling disappeared, the pigment gradually accumulated, and a tawny film subsequently formed in the wound. The healing period of the wound occurred 24 h after autotomy, and the blastema formation stage occurred 48 h after autotomy. After 4 days of cutting, the limb buds began to differentiate, grow, and expand rapidly, and this process lasted approximately 15 days. Microscopic observations revealed significant changes in the type and number of associated cells including outer epithelial cells, granulocytes, embryonic cells, columnar epidermal cells, elongated cells, and blastoma cells, during the process from limb fracture to regeneration. A comparative transcriptome analysis identified 1415 genes differentially expressed between the J0h (0 h post autotomy) and J18h (18 h post autotomy), and 3952 and 4366 differentially expressed genes for J0 and J14d (14 days post autotomy) and J18h and J14d, respectively. Some of these genes may be related to muscle growth or molting, as indicated by the presence of troponin C, chitinase, actin, innexin, and cathepsin L. As a functional gene involved in epidermal formation, the mRNA expression level of the innexin inx2 in the pereopod of E. carinicauda changed significantly in the experimental groups (p < 0.05). The results of this study contribute to existing knowledge of regeneration mechanisms in crustaceans.

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Comparative transcriptome and gut microbiota analysis of Exopalaemon carinicauda with different growth rates from a full-sib family

Cited by 2 publications

References 49 publications

Muscle Transcriptome Analysis Reveals Molecular Mechanisms of Superior Growth Performance in Kuruma Shrimp, Marsupenaeus japonicus

Muscle Transcriptome Analysis Reveals Molecular Mechanisms of Superior Growth Performance in Kuruma Shrimp, Marsupenaeus japonicus

Morphological and Molecular Changes during Limb Regeneration of the Exopalaemon carinicauda

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