Comparative Transcriptome Profiling Reveals Defense-Related Genes Against Ralstonia solanacearum Infection in Tobacco

Abstract

Bacterial wilt (BW) caused by Ralstonia solanacearum (R. solanacearum), is a vascular disease affecting diverse solanaceous crops and causing tremendous damage to crop production. However, our knowledge of the mechanism underlying its resistance or susceptibility is very limited. In this study, we characterized the physiological differences and compared the defense-related transcriptomes of two tobacco varieties, 4411-3 (highly resistant, HR) and K326 (moderately resistant, MR), after R. solanacearum infection at 0, 10, and 17 days after inoculation (dpi). A total of 3967 differentially expressed genes (DEGs) were identified between the HR and MR genotypes under mock condition at three time points, including1395 up-regulated genes in the HR genotype and 2640 up-regulated genes in the MR genotype. Also, 6,233 and 21,541 DEGs were induced in the HR and MR genotypes after R. solanacearum infection, respectively. Furthermore, GO and KEGG analyses revealed that DEGs in the HR genotype were related to the cell wall, starch and sucrose metabolism, glutathione metabolism, ABC transporters, endocytosis, glycerolipid metabolism, and glycerophospholipid metabolism. The defense-related genes generally showed genotype-specific regulation and expression differences after R. solanacearum infection. In addition, genes related to auxin and ABA were dramatically up-regulated in the HR genotype. The contents of auxin and ABA in the MR genotype were significantly higher than those in the HR genotype after R. solanacearum infection, providing insight into the defense mechanisms of tobacco. Altogether, these results clarify the physiological and transcriptional regulation of R. solanacearum resistance infection in tobacco, and improve our understanding of the molecular mechanism underlying the plant-pathogen interaction.

Silicon Application for the Modulation of Rhizosphere Soil Bacterial Community Structures and Metabolite Profiles in Peanut under Ralstonia solanacearum Inoculation

“…Seeds were grown in a glasshouse with natural light. A strain of R. solanacearum with a strongly aggressive defoliation was applied to the experiment of peanut inoculation [ 56 ]. TTC solid medium was used to revitalize the storage of glycerol (final concentration 25%) of R. solanacearum , stored in an ultra-cold storage freezer, and incubated at 28 °C for 48–72 h [ 4 ].…”

Induced defense strategies of plants against Ralstonia solanacearum

“…Plant-R. solanacearum associated transcriptomic data suggest the involvement of the cell wall in response to R. solanacearum infestation (Supplementary Table S1). For example, many upregulated genes in resistant tobacco 4,411-3 are involved in cell wall macromolecular metabolic processes and cell wall organization or biogenesis after inoculation with R. solanacearum (Pan et al, 2021). In peanut, cell wall-related genes showed specific expression differences between resistant and susceptible peanuts (Chen et al, 2014).…”

Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars

“…This suggestion was supported by Li et al (2021), who demonstrated that glutathione metabolism and phenylpropanoid pathways are the primary resistance pathways to Rs infection in tobacco. DEGs in glutathione metabolism were detected in the stems of a highly Rs-resistant tobacco variety as well (Pan et al, 2021). Glutathione is the most abundant antioxidant in cells and protects cell membranes and biomolecules from damage caused by reactive oxygen species (Dorion et al, 2021).…”