Comparative transcriptomic analysis of shrimp hemocytes in response to acute hepatopancreas necrosis disease (AHPND) causing Vibrio parahemolyticus infection

Zheng, Zhihong; Wang, Fan; Aweya, Jude Juventus; Li, Ruiwei; Yao, Defu; Zhong, Min; Li, Shengkang; Zhang, Yueling

doi:10.1016/j.fsi.2017.12.032

Cited by 51 publications

(39 citation statements)

References 20 publications

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“…Furthermore, hemocytes response during AHPND has shown activation of the apoptosis gene CAS2 in L . vannamei [81]. Our RNA-seq and gene expression analysis shows up-regulation of LvCAS , suggesting that the apoptosis process is an essential mechanism response to AHPND.…”

Section: Discussionmentioning

confidence: 86%

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Transcriptomic analysis of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) in response to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Velázquez-Lizárraga¹,

Juárez-Morales²,

Racotta³

et al. 2019

PLoS ONE

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Acute hepatopancreatic necrosis disease (AHPND), caused by marine bacteria Vibrio Parahaemolyticus , is a huge problem in shrimp farms. The V . parahaemolyticus infecting material is contained in a plasmid which encodes for the lethal toxins PirAB Vp , whose primary target tissue is the hepatopancreas, causing sloughing of epithelial cells, necrosis, and massive hemocyte infiltration. To get a better understanding of the hepatopancreas response during AHPND, juvenile shrimp Litopenaeus vannamei were infected by immersion with V . parahaemolyticus . We performed transcriptomic mRNA sequencing of infected shrimp hepatopancreas, at 24 hours post-infection, to identify novel differentially expressed genes a total of 174,098 transcripts were examined of which 915 transcripts were found differentially expressed after comparative transcriptomic analysis: 442 up-regulated and 473 down-regulated transcripts. Gene Ontology term enrichment analysis for up-regulated transcripts includes metabolic process, regulation of programmed cell death, carbohydrate metabolic process, and biological adhesion, whereas for down-regulated transcripts include, microtubule-based process, cell activation, and chitin metabolic process. The analysis of protein- protein network between up and down-regulated genes indicates that the first gene interactions are connected to oxidation-processes and sarcomere organization. Additionally, protein-protein networks analysis identified 20-top highly connected hub nodes. Based on their immunological or metabolic function, ten candidate transcripts were selected to measure their mRNA relative expression levels in AHPND infected shrimp hepatopancreas by RT-qPCR. Our results indicate a close connection between the immune and metabolism systems during AHPND infection. Our RNA-Seq and RT-qPCR data provide the possible immunological and physiological scenario as well as the molecular pathways that take place in the shrimp hepatopancreas in response to an infectious disease.

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Section: Discussionmentioning

confidence: 86%

“…As mentioned, several studies have looked at the pathogen- host response in shrimp using either different shrimp species or pathogens [5,12,15,75,81]. However, very few studies have investigated the shrimp immunological response at the transcriptome level.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) in response to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Velázquez-Lizárraga¹,

Juárez-Morales²,

Racotta³

et al. 2019

PLoS ONE

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“…olivacea 34 , Carcinus maenas 35,36 , Gecarcinus lateralis 37–41 , P . sanguinolentus 42 , Charybdis feriatus 43 , Litopenaeus vannamei 44–48 , Macrobrachium rosenbergii 49–52 , M . nipponense 53,54 , Exopalaemon carinicauda 55–57 , Oratosquilla oratoria 58–60 , Homarus americanus 61 , and so on.…”

Section: Introductionmentioning

confidence: 99%

The Single-molecule long-read sequencing of Scylla paramamosain

Wan

Jia

Zou

et al. 2019

Sci Rep

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Scylla paramamosain is an important aquaculture crab, which has great economical and nutritional value. To the best of our knowledge, few full-length crab transcriptomes are available. In this study, a library composed of 12 different tissues including gill, hepatopancreas, muscle, cerebral ganglion, eyestalk, thoracic ganglia, intestine, heart, testis, ovary, sperm reservoir, and hemocyte was constructed and sequenced using Pacific Biosciences single-molecule real-time (SMRT) long-read sequencing technology. A total of 284803 full-length non-chimeric reads were obtained, from which 79005 high-quality unique transcripts were obtained after error correction and sequence clustering and redundant. Additionally, a total of 52544 transcripts were annotated against protein database (NCBI nonredundant, Swiss-Prot, KOG, and KEGG database). A total of 23644 long non-coding RNAs (lncRNAs) and 131561 simple sequence repeats (SSRs) were identified. Meanwhile, the isoforms of many genes were also identified in this study. Our study provides a rich set of full-length cDNA sequences for S . paramamosain , which will greatly facilitate S . paramamosain research.

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“…Understanding the host immune responses to pathogen infection is the foundation to develop a strategy for disease control and prevention. Next-generation sequencing technology has been widely applied to understand the molecular responses in shrimp against pathogens, such as WSSV [10], Taura syndrome virus (TSV) [11], Vibrio parahaemolyticus ( V.p ) [12]. Considerable transcripts involved in immune defense have been identified in specific tissues of disease-stressed shrimp.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection

Wang

Xiang

et al. 2019

BMC Genomics

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Background Functional communications between nervous, endocrine and immune systems are well established in both vertebrates and invertebrates. Circulating hemocytes act as fundamental players in this crosstalk, whose functions are conserved during the evolution of the main groups of metazoans. However, the roles of the neuroendocrine-immune (NEI) system in shrimp hemocytes during pathogen infection remain largely unknown. Results In this study, we sequenced six cDNA libraries prepared with hemocytes from Litopenaeus vannamei which were injected by WSSV (white spot syndrome virus) or PBS for 6 h using Illumina Hiseq 4000 platform. As a result, 3444 differentially expressed genes (DEGs), including 3240 up-regulated genes and 204 down-regulated genes, were identified from hemocytes after WSSV infection. Among these genes, 349 DEGs were correlated with innate immunity and categorized into seven groups based on their predictive function. Interestingly, 18 genes encoded putative neuropeptide precursors were induced significantly by WSSV infection. Furthermore, some genes were mapped to several typical processes in the NEI system, including proteolytic processing of prohormones, amino acid neurotransmitter pathways, biogenic amine biosynthesis and acetylcholine signaling pathway. Conclusions The data suggested that WSSV infection triggers the activation of NEI in shrimp, which throws a light on the pivotal roles of NEI system mediated by hemocytes in shrimp antiviral immunity. Electronic supplementary material The online version of this article (10.1186/s12864-019-5614-4) contains supplementary material, which is available to authorized users.

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Comparative transcriptomic analysis of shrimp hemocytes in response to acute hepatopancreas necrosis disease (AHPND) causing Vibrio parahemolyticus infection

Cited by 51 publications

References 20 publications

Transcriptomic analysis of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) in response to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

Transcriptomic analysis of Pacific white shrimp (Litopenaeus vannamei, Boone 1931) in response to acute hepatopancreatic necrosis disease caused by Vibrio parahaemolyticus

The Single-molecule long-read sequencing of Scylla paramamosain

Transcriptome analysis reveals the activation of neuroendocrine-immune system in shrimp hemocytes at the early stage of WSSV infection

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