Comparative transcriptomic analysis reveals the gene expression profiles in the liver and spleen of Japanese pufferfish (Takifugu rubripes) in response to Vibrio harveyi infection

Peng, Hongyu; Yang, Boxue; Li, Boyan; Cai, Zhonglu; Cui, Qianjin; Chen, Mingkang; Liu, Xia; Yang, Xu; Jiang, Chen

doi:10.1016/j.fsi.2019.04.304

Cited by 29 publications

(13 citation statements)

References 67 publications

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“…Here, we performed RNA-sequencing analysis of T. rubripes spleen and gill responding to V. harveyi infection. Peng et al (30) demonstrated that V. harveyi can alter the splenic transcriptome of T. rubripes; however, the effect of V. harveyi on the T. rubripes gill transcriptome remains unknown. Our study is the first to report the changes in the T. rubripes gill transcriptome after V. harveyi infection.…”

Section: Discussionmentioning

confidence: 99%

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RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection

et al. 2022

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Takifugu rubripes is commonly subjected to the disease-causing bacterium, Vibrio harveyi. However, the mechanism involved in the immune response of T. rubripes to V. harveyi infection is unclear. We conducted a transcriptomic analysis of the spleen and gill from T. rubripes infected with V. harveyi. We obtained 60,981,357 and 60,760,550 clean reads from the control and infected spleens, and 57,407,586 and 57,536,651 clean reads from the control and infected gills, respectively. We also identified 1,560 and 1,213 differentially expressed genes in the spleen and gill, respectively. Gene ontology analysis revealed that the most enriched biological process in both the spleen and gill was “immune response”. The most enriched Kyoto Encyclopedia of Genes and Genomes immune response–related pathways were the NOD-like receptor signaling pathway in the spleen and cytokine–cytokine receptor interaction in the gill. We found 10 candidate immune-related genes in the spleen and gill. These putative immune pathways and candidate genes will provide insight into the immune response mechanisms of T. rubripes against V. harveyi.

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Section: Discussionmentioning

confidence: 99%

“…Real-time quantitative (RT-q) PCR was performed to validate the sequencing analysis results on a StepOnePlus Real-Time PCR system (ABI, USA) using SYBR green I fluorescent dye. Gene expression levels were normalized to T. rubripes β-actin (30). Relative gene expression was calculated using the 2 − CT method (31).…”

Section: Real-time Quantitative Pcrmentioning

confidence: 99%

RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection

et al. 2022

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“…Therefore, it is suitable for industrial recirculating aquaculture (Williams et al., 2018). However, serious parasitic disease caused by high‐density breeding has hindered the long‐term development of aquaculture (Peng et al., 2019). Therefore, studying the immune‐related molecular responses of T. rubripes and D. labrax during C. irritans infection is crucial for understanding the molecular mechanisms and developing effective measures against parasitic diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Two recent studies have examined C. irritans infection in the large yellow croaker ( Larimichthys crocea ) by RNA‐seq transcriptome sequencing (Fei et al., 2016; Wang et al., 2016), and a transcriptome analysis of the orange‐spotted grouper ( Epinephelus coioides ) with C. irritans infection has been conducted (Hu et al., 2017). In addition, RNA‐seq of the Japanese Pufferfish infected by Vibrio harveyi has been reported (Peng et al., 2019). These data provide a frame of reference to enhance our understanding of the molecular mechanisms involved in the immunity of fish during infection with C. irritans (Fei & Qian, 2017).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis in Takifugu rubripes and Dicentrarchus labrax gills during Cryptocaryon irritans infection

Cheng

Xia

Liu

et al. 2020

Journal of Fish Diseases

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Takifugu rubripes and Dicentrarchus labrax are important commercial fish in China that are under serious threat from Cryptocaryon irritans. C. irritans is a ciliated obligate parasite that causes marine white spot disease and leads to heavy economic losses. We analysed the transcriptome in the gills of T. rubripes and D. labrax to compare differentially expressed genes (DEGs) and pathways during infection with C. irritans. In total, we identified 6,901 and 35,736 DEGs from T. rubripes and D. labrax, respectively. All DEGs were annotated into GO terms; 6,901 DEGs from T. rubripes were assigned into 991 sub‐categories, and 35,736 DEGs from D. labrax were assigned into 8,517 sub‐categories. We mapped DEGs to the KEGG database and obtained 153 and 350 KEGG signalling pathways from T. rubripes and D. labrax, respectively. Immune‐related categories included Toll‐like receptors, MAPK, lysosome, C‐type lectin receptor and NOD‐like receptor signalling pathways were significantly enriched pathways. In immune‐related signalling pathways, we found that AP‐1, P38, IL‐1β, HSP90 and PLA were significantly up‐regulated DEGs in T. rubripes, but P38 and PLA were significantly down‐regulated in D. labrax. In this study, transcriptome was used to analyse the difference between scaly and non‐scaly fish infection by C. irritans, which not only provided a theoretical basis for the infection mechanism of C. irritans, but also laid a foundation for effectively inhibiting the occurrence of this disease. Our work provides further insight into the immune response of host resistance to C. irritans.

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“…Coagulation and complement are two distinct systems with unique pathophysiological roles, and both serve as innate defense against external threats (Oikonomopoulou et al, 2012). Cytokine-cytokine receptor interaction pathway plays important roles in immune response and has been identified in several previous studies of teleost under bacterial or virus infection (Peng et al, 2019;Zhang et al, 2019). In cluster1, genes highly expressed in 0 h were collected.…”

Section: Identification Of Lncrnas In L Croceamentioning

confidence: 99%

Identification and Expression Analysis of Long Non-coding RNA in Large Yellow Croaker (Larimichthys crocea) in Response to Cryptocaryon irritans Infection

et al. 2020

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Comparative transcriptomic analysis reveals the gene expression profiles in the liver and spleen of Japanese pufferfish (Takifugu rubripes) in response to Vibrio harveyi infection

Cited by 29 publications

References 67 publications

RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection

RNA-Sequencing Analysis of the Spleen and Gill of Takifugu rubripes in Response to Vibrio harveyi Infection

Transcriptome analysis in Takifugu rubripes and Dicentrarchus labrax gills during Cryptocaryon irritans infection

Identification and Expression Analysis of Long Non-coding RNA in Large Yellow Croaker (Larimichthys crocea) in Response to Cryptocaryon irritans Infection

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