Comparative Transcriptomic and Metabolomic Analyses in Response to Cold in Tartary Buckwheat (<i>Fagopyrum tataricum</i>)

Jeon, Jin Pyeong; Jk, Kim; Wu, Qingping; Su, Park

doi:10.1101/278432

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…The cDNA was mixed with 2X Real-Time PCR Master Mix (BIOFACT Co., Daejeon, Korea) and run on a qRT-PCR device, CFX96 Real-Time System (Bio-Rad Laboratories, Hercules, CA, USA) for gene expression analysis. Phenylpropanoid pathway gene sequences were obtained from the transcriptomic data of F. tataricum in our laboratory, where DNA sequences were determined by the Illumina NextSeq500 sequencer [ 29 ]. The conditions for qRT-PCR were as follows: 95 °C for 10 min, 40 cycles with two steps: denaturation at 95 °C for 15 s, annealing and elongation at 58 °C ( FtC3H , FtHQT , FtANR , and FtACT ), 60 °C ( FtC4H ), 61 °C ( FtPAL , FtCHI , FtF3H , and FtANS ), 62 °C ( Ft4CL , FtCHS , FtDFR , and FtFLS2 ) for 65 s, and 95 °C for 10 s; the temperature was increased by 0.5 °C per minute from 60 °C to 95 °C for detection of the melting curve.…”

Section: Methodsmentioning

confidence: 99%

Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Choi

Sathasivam

Nguyen

et al. 2021

Plants

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Tartary buckwheat (Fagopyrum tataricum) is an important crop that belongs to the Polygonaceae family, whose roots have received considerable attention due to the presence of compounds with high nutritional and medicinal value. In this study, we aimed to develop an efficient protocol for the culture of adventitious (ARs) and hairy (HRs) roots on a half-strength Schenk and Hildebrandt (SH) medium containing different concentrations of the auxins, α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA). The highest percentage of root induction (91.67%) was achieved with 0.5 mg/L IAA, whereas the greatest number of roots was found in 1 mg/L IAA. In contrast, 0.1 mg/L IBA returned the longest roots. As expected, HRs were obtained from in vitro leaf explants infected with Agrobacterium rhizogenes R1000. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 11 phenolic pathway genes revealed that five genes (FtPAL, FtC3H, FtHQT, FtCHS, and FtANS) were highly expressed in HRs, whereas only four (FtC4H, FtFLS2, FtDFR, and FtANR), and three (Ft4CL, FtCHI, and FtF3H) were recognized in the ARs and seedling roots (SRs), respectively. HPLC analysis of phenolic compounds in different root cultures showed that the majority of the phenolic compounds (both individual and total) were significantly accumulated in the HRs. Principal component analysis (PCA) identified differences among the three root types, whereby HRs were separated from ARs and SRs based on the amount of phenolic compounds present. Analysis of the metabolic pathway revealed that among the identified metabolites, the 3, 2, and 1 pathways were associated with flavonoid, flavone and flavonol, and phenylpropanoid biosynthesis, respectively. Hierarchical clustering analysis and the heat map showed that the different root cultures presented unique metabolites.

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Section: Methodsmentioning

confidence: 99%

Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Choi

Sathasivam

Nguyen

et al. 2021

Plants

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“…Buckwheat is an annual eudicot plant belonging to the family Polygonaceae, genus Fagopyrum [1]. Tartary buckwheat ( Fagopyrum tataricum (L.) Gaertn) and common buckwheat ( Fagopyrum esculentum Moench) are the two main species of buckwheat [2], which are considered as alternative crops or minor cereals and are popular food in Asia and Europe [3,4]. Tartary buckwheat and common buckwheat are traditionally regarded as medicinal and food homologous crops, because their grains are characterized by high contents of starch and dietary fibre, and protein with an advantageous amino acid composition.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS

Yang

et al. 2019

Molecules

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Flavonoids from plants are particularly important in our diet. Buckwheat is a special crop that is rich in flavonoids. In this study, four important buckwheat varieties, including one tartary buckwheat and three common buckwheat varieties, were selected as experimental materials. The total flavonoid content of leaves from red-flowered common buckwheat was the highest, followed by tartary buckwheat leaves. A total of 182 flavonoid metabolites (including 53 flavone, 37 flavonol, 32 flavone C-glycosides, 24 flavanone, 18 anthocyanins, 7 isoflavone, 6 flavonolignan, and 5 proanthocyanidins) were identified based on Ultra Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry (UPLC-ESI-MS/MS) system. Through clustering analysis, principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA), different samples were clearly separated. Considerable differences were observed in the flavonoid metabolites between tartary buckwheat leaves and common buckwheat leaves, and both displayed unique metabolites with important biological functions. This study provides new insights into the differences of flavonoid metabolites between tartary buckwheat and common buckwheat leaves and provides theoretical basis for the sufficient utilization of buckwheat.

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Comparative Transcriptomic and Metabolomic Analyses in Response to Cold in Tartary Buckwheat (Fagopyrum tataricum)

Cited by 2 publications

References 47 publications

Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Analysis of Flavonoid Metabolites in Buckwheat Leaves Using UPLC-ESI-MS/MS

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