2013
DOI: 10.4161/mabs.25592
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Comparing CDRH3 diversity captured from secondary lymphoid organs for the generation of recombinant human antibodies

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Cited by 11 publications
(6 citation statements)
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“…The heavy chain variable region, and in particular, the heavy chain CDR3, are considered to be the most important determinants of recognition [17,18,55], exemplified by experiments in which HCDR3 sequences from an anti-lysozyme VHH antibody (VHH) [56] were transplanted into neocarzinostatin [57] and sfGFP [58], conferring lysozyme binding activity. HCDR3’s have even been harvested as diversity elements [59,60] and binders have been selected from libraries in which they provided the only diversity [18,58]. As a result, deep sequencing initially concentrated on HCDR3, expanding to VH as read lengths increased.…”
Section: Applicationsmentioning
confidence: 99%
“…The heavy chain variable region, and in particular, the heavy chain CDR3, are considered to be the most important determinants of recognition [17,18,55], exemplified by experiments in which HCDR3 sequences from an anti-lysozyme VHH antibody (VHH) [56] were transplanted into neocarzinostatin [57] and sfGFP [58], conferring lysozyme binding activity. HCDR3’s have even been harvested as diversity elements [59,60] and binders have been selected from libraries in which they provided the only diversity [18,58]. As a result, deep sequencing initially concentrated on HCDR3, expanding to VH as read lengths increased.…”
Section: Applicationsmentioning
confidence: 99%
“…By applying computational methods one could estimate how the diversity is influenced by various processes, such as aging [ 10 ], vaccination, and infection [ 26 ]. Diversity measures could also be used to compare the structure of T- and B-cell repertoires in samples derived from a variety of tissues and subjects [ 27 ].…”
Section: Introductionmentioning
confidence: 99%
“…Accordingly, in recent years there have been considerable efforts in coupling high-throughput sequencing with phage display. Initially, the potential of this approach was demonstrated by studying protein interactions in a high-throughput manner 21 22 23 followed by studies of antibody display libraries 24 25 26 27 28 . Recently, a gene-specific library was used to identify immunodominant regions of a vaccine antigen 29 .…”
mentioning
confidence: 99%