Comparing core needle to surgical biopsies in breast cancer for cell kinetic and ploidy studies

Daidone, Maria Grazia; Orefice, Sergio; Mastore, Marinella; Santoro, Giuseppe; Salvadori, B; Silvestrini, Rosella

doi:10.1007/bf01975202

Cited by 4 publications

(2 citation statements)

References 14 publications

(11 reference statements)

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“…The needle core biopsy specimens in this study with ER results disparate from those for the mastectomy specimens constituted fewer than 1% of the needle core biopsy specimens evaluated at our institution, which is similar to the high degree of correlation reported by other authors. [35][36][37][38][39][40][41] The importance of minimum formalin fixation times and properly fixed tissue are not novel topics. Authors repeatedly have made these points in the context of good histologic sections, 6,42-48 consistent and accurate immunohistochemical results, 3,13,18,24,25,[49][50][51][52][53][54][55][56][57][58][59][60][61] and optimum immunohistochemical image ER values.…”

Section: Discussionmentioning

confidence: 99%

Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma

et al. 2003

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To identify the minimum time necessary for consistent immunohistochemical estrogen receptor (ER) results in our laboratory, we evaluated results in timed fixation blocks and cases with disparate and similar needle core biopsy and partial mastectomy specimens. Tissue sections of 24 ER-positive, invasive breast carcinomas were fixed for 3, 6, 8, and 12 hours and 1, 2, and 7 days. ER values were quantified using the Q score (0-7). In timed fixation blocks, the mean Q score per block was 2.46 for blocks fixed for 3 hours, 5.75 for blocks fixed for 6 hours, and 6.70 for blocks fixed for 8 hours (P < .001). The difference between the case maximum and mean block Q scores was a plateau of almost 0 at 6 to 8 hours of formalin fixation. For needle core biopsy specimen fixation times, the means for specimens with ER-disparate and ER-similar results were 1.2 and 6.3 hours, respectively (P = .01). The minimum formalin fixation time for reliable immunohistochemical ER results is 6 to 8 hours in our laboratory, regardless of the type or size of specimen.

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Section: Discussionmentioning

confidence: 99%

Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma

et al. 2003

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“…Other studies have addressed similar issues for markers other than angiogenesis and reproducible results have been obtained for determinations of DNA ploidy, bcl-2, oestrogen receptor, c-erbB-2 and p53, which are dichotomously scored variables (Daidone et al, 1991;Jacobs et al, 1998). Jacobs et al failed to produce reproducible results for mvc and accuracy was not improved by increasing tumour area available for assessment by performing more core biopsies (Jacobs et al, 1998).…”

Section: Figurementioning

confidence: 87%

Reproducibility of microvessel counts in breast cancer specimens

et al. 1999

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Assessment of tumour vascularity in core biopsy specimens may be a useful predictor of response to primary therapy. This study addresses practical methodological issues regarding accuracy of tumour vascularity assessments in different breast cancer specimens. Issues addressed in the study are variation caused by (i) inherent observer variation in the method, (ii) tumour heterogeneity and (iii) previous surgical manipulation of tumours. Microvessel counts were performed by two observers on separate occasions and by two different observers. Counts were performed on core biopsies and tumour sections taken simultaneously ( n = 16) and with an intervening time interval ( n = 21). In addition core biopsies were obtained from the same tumour on two separate occasions ( n = 10). A highly significant correlation was found in counts performed by the same observers at different times and between two different observers. No significant correlation was found in counts of core biopsies and tumour sections taken either simultaneously or subsequently. No correlation was found between counts of sequential core biopsies. Study findings suggest that, although microvessel counts may be assessed reproducibly by the same and different observers, counts performed in core biopsies do not accurately reflect those of overall tumour, limiting their potential as predictive or prognostic markers. © 1999 Cancer Research Campaign

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Preoperative assessment of proliferative activity and hormonal receptor status in carcinoma of the breast: A comparison of needle aspiration and needle-core biopsies to the surgical specimen

et al. 1996

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Comparing core needle to surgical biopsies in breast cancer for cell kinetic and ploidy studies

Cited by 4 publications

References 14 publications

Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma

Minimum Formalin Fixation Time for Consistent Estrogen Receptor Immunohistochemical Staining of Invasive Breast Carcinoma

Reproducibility of microvessel counts in breast cancer specimens

Preoperative assessment of proliferative activity and hormonal receptor status in carcinoma of the breast: A comparison of needle aspiration and needle-core biopsies to the surgical specimen

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