Comparing functional metabolic effects of marginal and sufficient selenium supply in sheep

Humann-Ziehank, Esther; Renko, Kostja; Mueller, Andreas S.; Roehrig, Petra; Wolfsen, Jacqueline; Ganter, Martin

doi:10.1016/j.jtemb.2013.03.003

Cited by 22 publications

(24 citation statements)

References 42 publications

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“…However, the relation SePP : Se-GPx was 1.6:1 in ovine serum being quite similar to the study [24] using human serum (1.7:1). Se-GPx in serum, which was mainly determined in former studies as GPx activity, was found previously to vary broadly over time comparing groups consuming the same feeding strategy as well as over time for individual animals [4]. An earlier study [25] suggested that about 25% of total serum Se is associated with GPx.…”

Section: Se and Se Species In Serummentioning

confidence: 95%

“…The study was part of a comprehensive project, the general study design was presented previously [4]. Ten sheep were used for the examination presented here, briefly the study design was as follows: The adult Cameroon crossbreed sheep were fed either a total ration providing < 0.05 mg Se/ kg dry weight (dw, n=5, Se -) representing a marginal Se supplementation or a total ration providing 0.2 mg Se/kg dw (n=5; Se + ) representing a sufficient Se supplementation for sheep.…”

Section: Animals and Feeding Strategymentioning

confidence: 99%

“…Detailed data of the experimental animals were published earlier [4,16]. In brief, during the whole experiment the sheep showed no abnormalities of behaviour and general health.…”

Section: General Condition Of the Experimental Animalsmentioning

confidence: 99%

“…The main biological function of Se is believed to exist through its incorporation into selenoproteins, which happens mainly in the form of selenocystine residues as an integral constituent of reactive oxygen species detoxifying selenoenzymes such as glutathione peroxidases (GPx), thioredoxin reductases (TrxR) and selenoprotein P (SePP) [3]. Marginal Se supplementation leads to a decrease of the antioxydative enzyme activity, in the first instance obvious in cytosolic GPx activity [4], probably due to the low rank of GPx in the hierarchic regulation of selenoprotein expression [5].…”

Section: Introductionmentioning

confidence: 99%

“…The study presented here conducts this topic by Se speciation in paired ovine samples having sufficient vs. marginal nutritional Se supplementation levels. The results are a part of a comprehensive study with a main focus on metabolic changes comparing marginal and sufficient Se supplementation published previously [4].…”

Section: Introductionmentioning

confidence: 99%

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Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Humann-Ziehank

Ganter

Michalke

2016

Journal of Trace Elements in Medicine and Biology

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This study was performed to characterise selenium (Se) and Se species in cerebrospinal fluid (CSF) of sheep and its relation to the respective Se concentrations in serum. Paired samples from 10 adult sheep were used for the study. Five sheep were fed a diet with a marginal Se concentration of <0.05mg Se/kg diet dry weight (dw, Se(-)), and five animals were fed the same diet supplemented with sodium selenite revealing a concentration of 0.2mg Se/kg diet dw (Se(+)). The feeding strategy was conducted for two years; The results on metabolic effects were published previously. At the end of the feeding period, paired samples of serum and CSF were collected and analysed using ion exchange chromatography inductively coupled plasma-dynamic reaction cell-mass spectrometry (IEC-ICP-DRC-MS) technique for total Se concentration and concentrations of Se species. Albumin concentrations were analysed additionally. The feeding strategy caused significant differences (p<0.01) in serum Se concentrations with 33.1±5.11μg Se/l in the Se(-) group and 96.5±18.3μg Se/l in the Se(+) group, respectively. The corresponding total Se concentrations in CSF were 4.38±1.02μg Se/l and 6.13±1.64μg Se/l in the Se(-) and the Se(+) group, respectively, missing statistical significance (p=0.077). IEC-ICP-DRC-MS technique was able to differentiate the Se species selenoprotein P-bound Se (SePP), selenomethionine, glutathione peroxidase-bound Se (Se-GPx), selenocystine, thioredoxin reductase-bound Se, ovine serum albumin-bound Se (Se-OSA), SeIV and SeVI in ovine serum and CSF. Quantitatively, SePP is the main selenoprotein in ovine serum followed by Se-GPx. The CSF/blood ratio of albumin (QAlbumin) reflected a physiological function of the blood-CSF barrier in all sheep. QSe-species were higher than QAlbumin both feeding groups, supporting the hypothesis of local production of Se species in the brain. Significant positive regression lines for CSF vs. serum were found for albumin and Se-OSA only, suggesting a role of albumin to convey Se across the blood-CSF barrier. The ovine model, together with the IEC-ICP-DRC-MS technique to characterise the Se species, might be a worthwhile model for further studies as repeated sample collection as well as modification of the nutritional status is feasible and effective.

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Section: Se and Se Species In Serummentioning

confidence: 95%

Section: Animals and Feeding Strategymentioning

confidence: 99%

“…Detailed data of the experimental animals were published earlier [4,16]. In brief, during the whole experiment the sheep showed no abnormalities of behaviour and general health.…”

Section: General Condition Of the Experimental Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Humann-Ziehank

Ganter

Michalke

2016

Journal of Trace Elements in Medicine and Biology

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Effect of Inorganic Dietary Selenium Supplementation on Selenoprotein and Lipid Metabolism Gene Expression Patterns in Liver and Loin Muscle of Growing Lambs

Juszczuk-Kubiak

Bujko

Cymer

et al. 2015

Biol Trace Elem Res

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Effect of selenium (Se) supplementation on the selenoprotein and lipid metabolism gene expression patterns in ruminants, especially in lambs is not yet fully understood. The aim of study was to evaluate the effect of Se supplementation on the messenger RNA (mRNA) expression patterns of selected selenoproteins and genes related to lipid metabolism in growing lambs. The experiment was conducted on 48 Polish Merino lambs divided into two groups (n = 24): control (C)—lambs fed with a basal diet (BD) with no Se supplementation, and supplemented (S)—lambs fed with a BD, supplemented with 0.5 mg Se/kg as sodium selenate for 8 weeks. Expression of 12 selenoproteins and six genes related to lipid metabolism was analyzed in the liver and longissimus dorsi (LD) muscle of growing lambs by qPCR. Significant differences were found in the expression of GPX1, GPX2, SEPM, SEPW1, SEP15, SEPGS2, and TXNRD1 in the liver, and GPX1, SEPP1, SEPN1, SEPW1, SEP15, and MSRB1 in the LD muscle between S and C lambs. Se supplementation mainly upregulated SEPW1, SEP15 (P < 0.001; P < 0.01) mRNA expression in the liver, and GPX1, SEPP1, SEPN1, SEPW1 (P < 0.001; P < 0.01) in the muscle of S group. On the other hand, significant decrease in GPX2 (P < 0.01), SEPM (P < 0.001), and SEPHS2 (P < 0.01) mRNA expression levels were observed in the liver of S group of lambs. Se supplementation did not affect PON1, LXRα, and PPARα mRNA expression levels, but a significant increase in mRNA levels of APOE and LPL in the LD muscle (P < 0.05) as well as LPL (P < 0.05) in the liver were noticed in the group of Se supplemented lambs. Our study confirmed that, in lambs, similarly to other species, mRNA expression patterns of several selenoproteins highly depend on dietary Se levels, and their expression is ruled by hierarchical principles and tissue-specific mechanisms. Moreover, the study showed that changes Se intake leads to different levels of genes expression related with lipid metabolism.

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Effects of Different Dietary Selenium Sources on Antioxidant Status and Blood Phagocytic Activity in Sheep

Čobanová

Faix

Plachá

et al. 2016

Biol Trace Elem Res

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The objective of this experiment was to investigate the effects of feed supplementation with equivalent doses of selenium from sodium selenite (SS) or selenized yeast (SY) on Se deposition, selenoenzyme activity and lipid peroxidation in tissues as well as in bacterial and protozoal fractions of rumen contents in sheep. The phagocytic activity of monocytes and neutrophils in whole blood was also assessed after 3 months of dietary treatment. While animals in the control group were fed with unsupplemented basal diet (BD) containing only background Se (0.16 mg/kg DM), the diet of the other two groups (n = 6) consisted of identical BD enriched with 0.4 mg Se/kg DM either from SS or SY. Concentrations of Se in blood and tissues were found to be significantly increased in both supplemented groups. No response in Se deposition was recorded in the musculus longissimus dorsi of sheep given dietary SS. The intake of SY resulted in a significantly higher Se level in the blood, kidney medulla, skeletal muscles, heart, intestinal and ruminal mucosa than in the case of SS supplementation. No differences appeared between tissue Se contents in the liver and kidney cortex due to the source of added Se. Regardless of source, Se supplementation to feeds significantly increased the glutathione peroxidase (GPx) activity in blood and tissues except the kidney medulla and jejunal mucosa. Supplementation with SY resulted in significantly higher activity of thioredoxin reductase in the liver and ileal mucosa, and also reduced malondialdehyde content in the liver and duodenal mucosa. Dietary Se intake increased Se concentrations in the total rumen contents and bacterial and protozoal fractions. The accumulation of Se in rumen microbiota was associated with increased GPx activity. Phagocytic cell activity was enhanced by Se supplementation. Our results indicate that Se from both sources has beneficial effects on antioxidant status in sheep and can be utilized by rumen microflora.

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Comparing functional metabolic effects of marginal and sufficient selenium supply in sheep

Cited by 22 publications

References 42 publications

Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Selenium speciation in paired serum and cerebrospinal fluid samples of sheep

Effect of Inorganic Dietary Selenium Supplementation on Selenoprotein and Lipid Metabolism Gene Expression Patterns in Liver and Loin Muscle of Growing Lambs

Effects of Different Dietary Selenium Sources on Antioxidant Status and Blood Phagocytic Activity in Sheep

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