Comparing in vitro maturation rates in buffalo and cattle oocytes and evaluating the effect of cAMP modulators on maturation and subsequent developmental competence

Abdulkarim, Abdelrahman; Balboula, Ahmed Z.; Badr, M. A.; Bedir, Wael; Elmetwally, Mohammed A; Zaabel, Samy

doi:10.21608/mvmj.2021.204767

Cited by 1 publication

(1 citation statement)

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“…LncRNA have been reported to affect the development of vitrification at the GV stage in cattle [ 10 ]. Moreover, cAMP signalling [ 11 ], MPF, PI3K/Akt signalling [ 12 ] and fatty acid metabolism [ 13 ] are involved in regulation of accelerated maturation following meiotic arrest in cattle. This complex molecular network is considered to be important for the oocyte to resume meiosis, which in turn produces a fertilized oocyte.…”

Section: Introductionmentioning

confidence: 99%

Identification of unique transcriptomic signatures through integrated multispecies comparative analysis and WGCNA in bovine oocyte development

Zhang

Zhao

et al. 2023

BMC Genomics

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Background Cattle (Bos taurus) are a major large livestock, however, compared with other species, the transcriptional specificity of bovine oocyte development has not been emphasised. Results To reveal the unique transcriptional signatures of bovine oocyte development, we used integrated multispecies comparative analysis and weighted gene co-expression network analysis (WGCNA) to perform bioinformatic analysis of the germinal follicle (GV) and second meiosis (MII) gene expression profile from cattle, sheep, pigs and mice. We found that the expression levels of most genes were down-regulated from GV to MII in all species. Next, the multispecies comparative analysis showed more genes involved in the regulation of cAMP signalling during bovine oocyte development. Moreover, the green module identified by WGCNA was closely related to bovine oocyte development. Finally, integrated multispecies comparative analysis and WGCNA picked up 61 bovine-specific signature genes that participate in metabolic regulation and steroid hormone biosynthesis. Conclusion In a short, this study provides new insights into the regulation of cattle oocyte development from a cross-species comparison.

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Section: Introductionmentioning

confidence: 99%