Comparing modified and plain peptide linked enzyme immunosorbent assay (ELISA) for detection of human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2) antibodies

Manocha, Monika; Keisham, Chitralekha; Thakar, Madhuri; Shashikiran, D.; Paranjape, Ramesh; Rao, D. N.

doi:10.1016/s0165-2478(02)00257-2

Cited by 19 publications

(14 citation statements)

References 6 publications

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“…One of the main drawbacks is the difficulty of passive adsorption on the polystyrene titration plates (standard ELISA procedure), which can potentially be overcome by covalent coupling to proteins carrier [21], Cysteinyl-glycylglycine (CGG) moiety derivatisation [22], the use of derivatised peptides with hydrophobic tails of fatty acids that orientate and encourage the adsorption of the peptidic sequences on plates [23] or the covalent bonding of the peptide to the solid medium [24].…”

Section: Chemical Strategies To Overcome Synthetic Antigenic Peptide mentioning

confidence: 99%

“…Although biotin-avidin methodology has been widely applied in immunodiagnosis assays, a drop in sensitivity of biotinylated peptides due to the presence of biotin binding with avidin leading to the masking of peptide for recognition with antibodies has been reported [22].…”

Section: A Covalent Coupling Of Synthetic Peptides To Carrier Protmentioning

confidence: 99%

“…It seems that the presence of the CGG motif may act as a handle, thus presenting the antigen in its near native conformation for antibody binding. Also, although possible problems linked to presence of cysteine residues in a peptide, like masking of epitope in the polymer could arise, Manocha et al [22] highlighted the importance of the cysteine moiety that can lead to polymerization leading to a large number of epitopes accessible for antibody recognition. Thus CGG motif seems to provide a way of attaching the short peptides to ELISA plates with high binding efficiency thereby improving the sensitivity without sacrificing the specificity.…”

Section: B Chemical Derivatisation Of Synthetic Peptidesmentioning

confidence: 99%

“…Cysteinyl-glycyl-glycine (CGG) derivatization [22] Some peptide molecules only poorly adsorb to solid phases a relatively large part of the peptide being involved in the non-covalent adsorption. Usually peptides do not have the same conformation free in solution as when adsorbed or covalently coupled to microtiter plates, the orientation of an immobilized antigen being important for recognition by an antibody.…”

Section: Chemical Derivatization Of Synthetic Peptidesmentioning

confidence: 99%

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Synthetic Peptides for the Immunodiagnosis of Human Diseases

Gómara¹,

Haro

2007

CMC

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Synthetic peptides have been shown to be valuable tools for viral laboratory diagnosis and can provide uniform, chemically well-defined antigens for antibody analysis, reducing inter- and intra-assay variation. The main aim in the development of peptide-based diagnostic tests is to recognise specific antibodies induced by the whole viral proteins but using selected short fragments containing the most potent antigenic determinants. The success of this approach depends on the extent to which synthetic peptides are able to mimic the immunodominant epitopes of antigens. In recent years, synthetic peptides that mimic specific epitopes of infectious agents' proteins have been used in diagnostic systems for various human diseases. The present review summarizes some of the drawbacks of the use of relatively short linear peptides as antigenic substrates and the subsequent chemical strategies developed in order to overcome the low peptide reactivity against specific antibodies. Moreover, it outlines the most significant bibliography published in the last five years which provides validated peptide based tests potentially useful for diagnosis of viral, bacterial, parasitic and autoimmune diseases.

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Section: Chemical Strategies To Overcome Synthetic Antigenic Peptide mentioning

confidence: 99%

Section: A Covalent Coupling Of Synthetic Peptides To Carrier Protmentioning

confidence: 99%

Section: B Chemical Derivatisation Of Synthetic Peptidesmentioning

confidence: 99%

Section: Chemical Derivatization Of Synthetic Peptidesmentioning

confidence: 99%

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Synthetic Peptides for the Immunodiagnosis of Human Diseases

Gómara¹,

Haro

2007

CMC

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“…Therefore, 100% clinical specificity was obtained for both polypeptides. The 100% specificity of the ELISA-HIV2 assay compares favorably to the specificity of the two licensed HIV-2 serodiagnosis assays (Ͻ92%) (2, 7) and to the specificity of most mixed HIV-1/HIV-2 assays (mean, 99%; range, 94.6% to 100% for assays based on recombinant proteins; mean, 98%; range 90.4% to 100% for assays based on synthetic peptides) (1,4,5,12,14,22,28,31,38,42,43,45,48).…”

mentioning

confidence: 94%

Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

et al. 2006

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A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

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