Comparing Pig and Amphibian Oocytes: Methodologies for Aneuploidy Detection and Complementary Lessons for MAPK Involvement in Meiotic Spindle Morphogenesis

Ješeta, Michal; Bodart, Jean‐François

doi:10.5772/34070

Cited by 2 publications

(2 citation statements)

References 102 publications

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“…Meiosis III-type phenomena have been observed in mouse, bovine, plant and ascidian meioses [17][18][19][20]. In mouse and ascidian meioses, proper regulation of Mos-MAPK activity is essential to avoid the entry to meiosis III [19,21,22], although this is unlikely to be a universal mechanism according to studies in other species, including amphibians [23]. In Arabidopsis thaliana, initiation of meiosis II is regulated by a close homologue of fission yeast Mes1 [24].…”

Section: The Mechanism Of Terminating the Meiotic Cyclementioning

confidence: 99%

Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle

et al. 2013

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The number of nuclear divisions in meiosis is strictly limited to two. Although the precise mechanism remains unknown, this seems to be achieved by adjusting the anaphase-promoting complex/cyclosome (APC/C) activity to degrade cyclin. Here, we describe a fission yeast cuf2 mutant that enters into a third nuclear division cycle, represented by ectopic spindle assembly and abnormal chromosome segregation. Cuf2 is a meiotic transcription factor, and its critical target is fzr1 þ /mfr1 þ , which encodes a meiotic APC/C activator. fzr1D also enters a third nuclear division. Thus, Cuf2 ensures termination of the M-phase cycle by boosting Fzr1 expression to generate functional gametes.

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Section: The Mechanism Of Terminating the Meiotic Cyclementioning

confidence: 99%

Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle

et al. 2013

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“…Regarding meiosis resumption, the depletion of CBS by siRNA in mice oocytes was shown to prevent this process, but the siRNA was done in granulosa cells and not directly in oocytes. In porcine oocytes, while the main characteristics of meiosis appeared to be similar to Xenopus oocytes (dependence upon protein synthesis, MPF and MAPK pathways activation, meiotic resumption independent of MAPK activation [69]), responses towards perturbations driven by H 2 S differ. CBS, CSE, and MPST were detected in immature and mature Xenopus oocytes, whereas these enzymes exhibit a different profile of expression in porcine oocytes regarding their maturation state [21].…”

Section: Discussionmentioning

confidence: 93%

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

Gelaude

Slaby

Cailliau

et al. 2020

Cells

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The role of hydrogen sulfide (H2S) is addressed in Xenopus laevis oocytes. Three enzymes involved in H2S metabolism, cystathionine β-synthase, cystathionine γ-lyase, and 3-mercaptopyruvate sulfurtransferase, were detected in prophase I and metaphase II-arrested oocytes and drove an acceleration of oocyte meiosis resumption when inhibited. Moreover, meiosis resumption is associated with a significant decrease in endogenous H2S. On another hand, a dose-dependent inhibition was obtained using the H2S donor, NaHS (1 and 5 mM). NaHS impaired translation. NaHS did not induce the dissociation of the components of the M-phase promoting factor (MPF), cyclin B and Cdk1, nor directly impacted the MPF activity. However, the M-phase entry induced by microinjection of metaphase II MPF-containing cytoplasm was diminished, suggesting upstream components of the MPF auto-amplification loop were sensitive to H2S. Superoxide dismutase and catalase hindered the effects of NaHS, and this sensitivity was partially dependent on the production of reactive oxygen species (ROS). In contrast to other species, no apoptosis was promoted. These results suggest a contribution of H2S signaling in the timing of amphibian oocytes meiosis resumption.

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Comparing Pig and Amphibian Oocytes: Methodologies for Aneuploidy Detection and Complementary Lessons for MAPK Involvement in Meiotic Spindle Morphogenesis

Cited by 2 publications

References 102 publications

Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle

Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle

Hydrogen Sulfide Impairs Meiosis Resumption in Xenopus laevis Oocytes

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