Rheological test standards require asphalt samples, both original and under ageing conditions. The most common laboratory equipment in specifications for short-term and long-term ageing simulation tests are the rolling thin film oven (RTFO) and the pressure ageing vessel (PAV). However, the cost of acquiring PAV and the duration of long-term ageing tests can be a limiting factor. This work aimed to establish the equivalent time of the long-term ageing test in the RTFO that corresponds to the PAV. For this, the Brazilian asphalt PEN 50/70, specified by penetration, was aged at different times (85, 170, 255, and 340 min) in the RTFO at the standard temperature (163 °C). For each time, using a dynamic shear rheometer (DSR), tests such as Multiple Stress Creep Recovery (MSCR) and Linear Amplitude Sweep (LAS) were performed, and the rheological properties (complex modulus (G*) and phase angle (δ)) were measured. The same tests were conducted on the samples aged in the long term and in the PAV. The test parameters obtained from applying different times while using the RTFO were compared with the PAV results, and the equivalent time was settled through linear regression, resulting in 300 min. In order to confirm the equivalent time, samples aged in the RTFO for 300 min were assessed using the same rheological tests, and the parameters were compared to those obtained after PAV ageing. At the equivalent time, the difference between RTFO and the PAV for the rutting parameter (G*/sinδ, 58 °C) was 6%, while for the fatigue parameter (G*.sinδ, 19 °C), the difference was 1.0%. The MSCR non-recoverable creep compliance parameter differences, considering stress levels of 0.1 kPa and 3.2 kPa, were 9.7% and 11.7%, respectively. For the fatigue life obtained in the LAS test at strain levels of 1.25% and 2.5%, the difference between RTFO and PAV, at the equivalent time, was 7.6% and 7.8%, respectively. For the Brazilian unmodified asphalt PEN 50/70 and parameters evaluated in this work, 300 min is the equivalent time that simulates long-term ageing in the RTFO.